Quality Changes and Pasteurization Effects of Citrus Fruit Juice by High Voltage Pulsed Electric Fields (PEF) treatment

고전압 펄스 전기장 처리에 의한 감귤주스의 품질변화

  • Published : 2003.08.01

Abstract

A non-thermal pasteurization technology, high Pulsed Electric Field (PEF) has been thought to be a new alternative processing technology instead of heating. The objective of this study was to examine and compare the effect of PEF and High Temperature Short Time (HTST) treatments on the physicochemical, microbiological and sensory characteristics of citrus juices. Total sugar and titratable acidity values of fresh citrus juice and two treatments were not significantly different each other at p<0.05. The concentration of vitamin C in fresh citrus juice $(31.2{\pm}0.59\;mg%)$ was not significantly different with the value of PEF treatment $(29.4{\pm}0.75\;mg%)$ but was significantly higher than the value of HTST treatment $(27.4{\pm}0.75\;mg%)$. The color values (L, a, and b) in PEF treatment were significantly lower than the fresh citrus juice, but were higher than the values of HTST treatment. Both total bacterial cell counts $(6.65\;{\pm}\;0.08\;log_{10}(cfu/mL))$ and yeast counts $(7.79{\pm}0.07\;log_{10}(cfu/mL))$ in fresh citrus juice were significantly reduced by PEF $(1.39{\pm}0.14,\;2.42{\pm}0.1\;log_{10}(cfu/mL))$ as well as HTST treatment (0, 0). PE activity of fresh citrus juice $(1.3{\pm}0.12\;units/mL)$ was significantly reduced by PEF treatment $(0.11{\pm}0.01\;units/mL)$ and was totally inactivated by HTST treatment. Sensory evaluation scores in flavor, taste and overall acceptability between the fresh and PEF treated citrus juices $(7.2{\sim}7.5)$ were not significantly different but the values of HTST treatment $(5.1{\sim}5.8)$ were lower than others. Consequently, PEF treatment is thought to be a good alternative pasteurization method for fresh citrus juice to HTST treatment due to its strong pasteurization effect, reduced destruction of nutrients and good sensory characteristics.

Keywords

PEF;HTST;non-thermal pasteurization;citrus fruit juice;microorganism

References

  1. Baker, R.A. and Bruemmer, J.H. Pectinase stabilization of orange juice cloud. J. Agric. Food Chem. 20: 1169-1975 (1972) https://doi.org/10.1021/jf60184a011
  2. Castro, A.J., Barbosa-Canovas, G.V. and Swanson, B.G. Inactivation of foods by pulsed electric field. J. Food Proc. Preser. 17: 47-73 (1993) https://doi.org/10.1111/j.1745-4549.1993.tb00225.x
  3. Tressler, D.K. Nutritive value of fruit and vegetable juices, pp. 447-453. In: Fruit and Vegetable Juice Processing Technology. Donald, K. and Maynard, A. (eds.). The AVI Publishing Co. Inc., Westport, USA (1961)
  4. Grahl, T, Sitzman, W. and Markl, H. Killing of microorganisms in fluid media by high-voltage pulses. Abstract presented at the 10th Dechema Annual Meeting of Biotechnologists, Karlsruhe, Germany (1992)
  5. Steven, L.H. High intensity pulsed electric fields and high hydrostatic pressure processing of apple juice. Ph.D. dissertation, Washington State Univ., USA (1997)
  6. Ho, S.Y., Mittal, G.S. and Cross, J.D. Effects of high electric field pulses on the activity of selected enzymes. J. Food Eng. 31: 69-84 (1997) https://doi.org/10.1016/S0260-8774(96)00052-0
  7. O'nahony, M.O. Sensory Evaluation of Food (Statistical Methods and Procedures), pp. 153-160. Marcel Dekker Inc., New York, USA (1985)
  8. Hofmann, G.A. Microflora Reduction in Liquids with Pulsed Electric Fields. Biotechnologies and Experimental Research, Inc., San Diego, USA (1984)
  9. Huxsoll, C.C. and Bolin, H.R. Processing and distribution alternatives for minimally processed fruits and vegetables. Food Technol. 42: .124-128 (1989)
  10. Shin, J.K. and Pyun, Y.R. Inactivation of Lactobacillus plantarum by pulsed-microwave irradiation. J. Food Sci. 62: 163-166 (1997) https://doi.org/10.1111/j.1365-2621.1997.tb04391.x
  11. Hawer, W.D, Ha, J.H., Seog, H.M., Nam, Y.J. and Shin, D.W. Changes in the taste and flavour compounds of kimchi during fermentation. Korean J. Food Sci. Technol. 20: 511-517 (1988)
  12. Dziezak, I.D. New process concentrates juices-preserving 'fresh notes'. Food Technol. 43: 148-149 (1989)
  13. Ha, S.D. Evaluation of dryfilm method for isolation of microorganisms from foods. Korean J. Appl. Microbiol. Biotechnol. 24: 178-184 (1996)
  14. Jia, M., Zang, O.H and Min, D.B. Effect of pulsed electric field processing on orange juice flavor analyzed by dynamic head space gas chromatography. Abstract presented at the IFT 1996 Annual Meeting, Orlando, USA (1996)
  15. Kim, K.O. and Lee, Y.C. Sensory Evaluation of Food, pp. 185-188. Hak Yeon Publishing Co., Seoul, Korea (1989)
  16. Zhang, Q., Barbosa-Canovas, G.V. and Swanson, B.G. Engineering aspects of pulsed electric field pasteurization. J. Food Eng. 25: 261-281 (1995) https://doi.org/10.1016/0260-8774(94)00030-D
  17. Hulsheger, H., Potel, J. and Niemann, E.G. Electric field effects on bacteria and yeast cells. Radial. Environ. Biophys. 22: 149-162 (1983) https://doi.org/10.1007/BF01338893
  18. Owusu-Yaw. J., Marshall, M.R., Koburger, J.A. and Wei, C.I. Low pH inactivation of pectinesterase in single strength juice. J. Food Sci. 53: 504-507 (1988) https://doi.org/10.1111/j.1365-2621.1988.tb07742.x
  19. Crandall, P.G., Mattews, R.F. and Baker, R.A. Citrus beverage clouding agents-review and status. Food Technol. 37: 106-110 (1983)
  20. Pothakamury, U.R., Vega, H., Zang, Q., Barbosa-Canovas, G. and Swanson, B.G. Effect of growth stage and processing temperature on the inactivation of E. coli by pulsed electric fields. J. Food Prot. 59: 1167-1171 (1996) https://doi.org/10.4315/0362-028X-59.11.1167
  21. Ma, S.J., Cho, G.S., Joo, H.K., Park, C.K., Chae, I.K. and Cho, H.Y. Food Analysis Method, pp. 302-304. Hak Moon Publishing Co., Seoul, Korea (1996)
  22. Kimball, D. Citrus Processing, pp. 117-125. AVI, New York, USA (1991)
  23. Qin, B.L., Pothakamury, U.R., Vega, H., Martin, O., Barbosa-Canovas, G.V. and Swanson, B.G. Food pasteurization using high-intensity pulsed eiectric fields. Food Technol. 49: 55-60 (1995)
  24. Ohlsson, T. Minimal processing-preservation methods of the future. Trends Food Sci. Technol. 5: 341-344 (1994) https://doi.org/10.1016/0924-2244(94)90210-0
  25. Jung, D.H and Jang, H.G. Food Anslysis, pp. 283-285. Jin Ro Youn Gu Sa, Seoul, Korea (1979)
  26. Vega-Mercado, H., Powers, J.R., Barbosa-Canovas, G,V., Swanson, B.G. and Luedecke, L. Inactivation of a protease from Pseudomonas fluorescens M3/6 using high volage pulsed electric fields. Abstract presented at the IFT 1995 Annual Meeting, Los Angeles, USA (1995)
  27. Hulsheger, H., Potel, J. and Niemann, E.G. Killing of bacteria with electric pulses of high field strength. Radiat. Environ. Biophys. 20: 53-65 (1981) https://doi.org/10.1007/BF01323926
  28. Kim, KT., Kim, S.S., Choi, H.D., Hong, H.D., Ha, S.D. and Lee, Y.C. Physiological properties of microbial cells treated by pulsed electric field (PEF). Korean J. Food Sci. Technol. 31: 368-374 (1999)
  29. Zhang, Q., Qin, B.L., Barbosa-Canovas, G.V. and Swanson, B.G. Inactivation of E. coli for food pasteurization by high-intensity-short- duration pulsed electric fields. J. Food Proc. Preser, 19: 103-118 (1994)
  30. Kalchayanand, N., Sikes, T., Dunne, C.P. and Ray, B. Hydrostatic pressure and electroporation have increased bactericidal efficiency in combination with bacteriocins. Appl. Environ. Microbiol. 60: 4174-4177 (1994)
  31. Joo, H.K., Cho, K.S., Cho, S.K., P, C.K and Ma, S.C. Analysis Method of Food, pp. 355-358. Yoo Lim Publishing Co., Seoul, Korea (1989)