Discriminating Domestic Soybeans from Imported Soybeans by 20 MHz Pulsed NMR

20 MHz pulsed NMR을 이용한 국내산과 수입산 콩의 판별

  • Published : 2003.08.01

Abstract

A 20 MHz pulsed NMR systems was employed to discriminate the geographical origin of soybeans and black beans (yak-kong) from Korea and foreign countries. Crude fat contents measured by soxhlet method were significantly (p<0.05) different between domestic and imported soybeans. Moisture and crude protein contents, measured by AOAC, were significantly different between domestic and imported black beans. In soybeans, values by solid fat content method and Carr-Purcell-Meiboom-Gill (CPMG) method using 20 MHz pulsed NMR showed the significant difference among soybeans from various the geographical origins. In black beans (yak-kong), NMR values measured by NMR except $T_1$ SR pulse sequence revealed the significant difference by the geographical origins. The habitat of soybeans and black beans could be identified by canonical discriminant analysis of chemical composition with $70{\sim}91.7\;%$ accuracy. Low field NMR data followed by discriminant analysis, however, granted the 100% of accuracy for classification of soybeans.

Keywords

20 MHz pulsed NMR;soybean;black bean (yak-kong);origin;canonical discriminant analysis

References

  1. Kwon, Y.K. and Cho, R.K. Identification of geographical origin of sesame seeds by near infrared spectroscopy. J. Korean Agric. Chem. 41: 240-246 (1998)
  2. Noh, B.S. and Ko, J.w. Discriminant of the habitat for agricultural products by using electronic nose. Food Eng. Prog. 1: 103-106 (1997)
  3. Noh, S.H. Non-destructive technology for quality evaluation of agricultural products. Current status and perspectives of industrial food engineering. 65-90 (1995)
  4. AOAC. Official Methods of Analysis. 15th ed. The Association of Official Analytical Chemistry. Washington, D.C., USA (1990)
  5. Kim, Z.U. and Byun, S.M. Studies on the protein of Korean soybeans. Part 1; Chemical compositions of Korean soybeans. Korean J. Agric. Chem. 7: 79-84 (1966)
  6. Kim, J.G., Kim, S.K. and Lee, J.S. Fatty acid composition and electrophoretics pattern of protein of Korean soybeans. Korean J. Food Sci. Technol. 20: 263-271 (1988)
  7. Rho, J.H. and Lee, S.M. Discriminating the geographical origin of sesame seeds by low field NMR. Korean J. Food Sci. Technol. 34: 1062-1066 (2002)
  8. Kim, Y.S., Scotter, C.N.G., Voyiagis, M.N. and Hall, M. Potential of NIR spectroscopy for discriminating geographical origin of green tea from Korea and Japan. Foods Biotechnol. 6: 74-80 (1997)
  9. Noh, B.S., Ko, J.W., Kim, S.Y. and Kim, S.J. Application of electronic nose in discrimination of the habitat for special agricultural products. Korean J. Food Sci. Technol. 30: 1051-1057(1998)
  10. Kim, Y.S., Scotter, C.N.G., Voyiagis, M. and Hall, M. Potential of NIR spectroscopy for discriminating geographical origin of sesame oil. Food Sci. Biotechnol. 7: 18-22 (1998)
  11. Kim, S.M. Magnetic resonance techniques and food engineering. Food Eng. Prog. 5: 258-265 (2001)
  12. SAS institute, Inc. SAS User's Guide. Statistical Analysis System institute, 5th ed., Cary, NC, USA (1985)
  13. Jeong, S.S., Cho, K.K., Choi, S.Y. and Yoon, J.S. Studies on the identification of geographical origins of agricultural products. Experiment Research Institute of National Agricultural Products Quality Management Service Report 41-46 (1996)
  14. Cho, Y.S., Han, K.Y., Kim, J.H., Kim, S.J. and Noh, B.S. Application of electronic nose in discrimination of the habitat for black rice. Korean J. Food Sci. Technol. 34: 136-139 (2002)
  15. Kim, Y.H., Yun, H.T., Chung, W.K. and Kim, S.D. Determination of seed component contents in whole soybean seed by near infrared spectroscopic analysis. RDA. J. Agric. Sci. 37:91-94(1995)
  16. National Agricultural Statistics Service. Statistical Report. 30, June, Seoul, Korea (2000)
  17. Barker, P.J. Low Resolution Analytical NMR. Bruker Application Note 30 (1987)