A Caspase Inducing Inhibitor Isolated from Caesalpinia sappan

소목(Caesalpinia sappan)으로부터 분리한 caspase 유도 저해 물질

  • Published : 2003.08.01

Abstract

Through the screening of caspase-3 inducing inhibitors in U937 human monocytic leukemia cell from natural sources, Caesalpiniae sappan, which showed a high level of inhibition, was selected. The inhibition compound was purified from methanol extract by silica gel column chromatography and HPLC. The inhibitor was identified as brazilin by spectroscopic methods of ESI-MS, $^1H-NMR$, and $^{13}C-NMR$. Brazilin showed inhibitory activity of caspase-3 induction, a major protease of apoptosis cascade, with $IC_{50}$ value of $4.5\;{\mu}g/mL$ after 7 hr of treatment in U937 cells.

Keywords

apoptosis;caspase-3 inhibitor;brazilin;U937

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