찔레 영지버섯(Phellinus ribis) 추출물의 생리활성

Physiological Activities of Phellinus ribis Extracts

  • 송재환 (성균관대학교 생명공학부) ;
  • 이현숙 (성균관대학교 생명공학부) ;
  • 황진국 (성균관대학교 생명공학부) ;
  • 정태영 (성균관대학교 생명공학부) ;
  • 홍성렬 (성균관대학교 생명공학부) ;
  • 박기문 (성균관대학교 생명공학부)
  • Song, Jae-Hwan (Faculty of Life Science and Technology, Sungkyunkwan University) ;
  • Lee, Hyun-Sook (Faculty of Life Science and Technology, Sungkyunkwan University) ;
  • Hwang, Jin-Kook (Faculty of Life Science and Technology, Sungkyunkwan University) ;
  • Chung, Tae-Young (Faculty of Life Science and Technology, Sungkyunkwan University) ;
  • Hong, Sung-Ryul (Faculty of Life Science and Technology, Sungkyunkwan University) ;
  • Park, Ki-Moon (Faculty of Life Science and Technology, Sungkyunkwan University)
  • 발행 : 2003.08.01

초록

Phellinus ribis(찔레 영지버섯)는 약용버섯으로 일부 항암 및 관절염 치료에 사용되고 있으나 그 활성이 전혀 알려져 있지 않아 1차적으로 40% ethanol 추출물의 생리활성을 확인하였다. 항산화 활성에 있어 nitrosamine의 원인물질인 nitrite 제거활성은 pH 1.2에서 0.5 mg/mL 이하 농도에서는 50% 이하, 그리고 1 mg/mL 처리 시 $64.0{\pm}1.6%$의 제거활성을 나타냈으며, 농도 의존적으로 증가하였다. DNA 손상 및 암, 당뇨, 간경화증, 심혈관질환 등의 원인물질에 관련하여 DPPH radicals의 제거활성은 0.5 mg/mL에서 $62.5{\pm}0.3%$, 1 mg/mL 처리 시 $91.3{\pm}0.8%$로 높게 나타났다. 2.5% linoleic acid의 자동산화에 미치는 항산화 활성을 측정한 결과 0.01 mg/mL 처리 시 p<0.05 수준에서, 0.5 및 1 mg/mL에서는 p<0.001 수준에서 유의성 있게 산화를 억제하였으며, superoxide dismutase 유사활성은 $530{\pm}81\;unit/g$으로 나타났다. 그리고, 항고혈압에 관련된 angiotensin converting enzyme 저해 활성은 $12.0{\pm}1.5%$로 그 활성이 미약하였다. Human 유래 암세포에 대한 세포독성 실험결과 폐암세포인 A549의 경우 100 mg/mL 농도에서도 16%로 나타나 세포독성이 미약하였고, 자궁암 세포인 HeLa의 경우 50 mg/mL에서 45%의 효과를, 위암 세표인 AGS에서는 5 mg/mL 처리 시 24%, 50 mg/mL에서는 76%, 그리고 간암 세포주인 SK-Hep-1의 경우 50 mg/mL에서 42%의 세포독성을 나타내었다. Salmonella typhimurium TA98 및 TA100에 대한 돌연변이원성 시험결과 찔레 영지버섯 추출물은 자연복귀 집락보다 histidine revertant colony 수가 2배 이상 증가하지 않았고, 추출물의 첨가농도를 증가시켜도 histidine revertant colony 수가 증가하지 않아 돌연변이성이 없었다.

참고문헌

  1. Park, J.G., Hyun, J.W, Lim, K.H., Shin, J.E., Won, Y.J., Yi, Y.D., Shin, K.H., Chang, I.M. and Woo, W.S. Antineoplastic effects from traditional medicinal plants. Korean J. Pharmacol. 24: 223-230 (1993)
  2. Chung, K.S., Toon, K.D., Kwon, D.J., Hong, S.S. and Choi, S.Y. Cytotoxicity testing of fermented soybean products with various tumour cells using MTT assay. Korean J. Appl. Microbiol. 25: 477-482 (1997)
  3. Park, Y.D., Hong, Y.K., Whang, W.K., Huh, J.D., and Park, S. Comparisons of protein-bound polysaccharide contents obtained from mycelial cultured broth and fruit body of Coriolus versicolor. Korean J. Mycol. 17: 223-228 (1989)
  4. Maeda, Y. and Chihara. H. Lentinan, a new immunoaccelerator of cell mediated response. Nature 229: 634-639 (1971) https://doi.org/10.1038/229634a0
  5. Dennert, G. and Tucker, D. Antitumor polysaccharide lentinan a T cell adjuvant. J. Natl. Cancer Inst. 51: 1727-1735(1973) https://doi.org/10.1093/jnci/51.5.1727
  6. Liu, F., Ooi, V.E.C., Liu, W.K. and Chang, S.T. Immunomodulation and antitumor activity of polysaccharide-protein complex from the culture filtrates of a local edible mushroom, Tricholoma lobayense. Gen. Pharmac. 27: 621-624 (1996) https://doi.org/10.1016/0306-3623(95)02058-6
  7. Kim, H.M., Han, S.B., Oh, G.T., Kim, Y.H., Hong, D.H. Hong, N.D. and Yoo, I.D. Stimulation of humoral and cell mediated immunity by polysaccharide from mushroom Phellinus linteus. Int. J. Immunopharmac. 18: 295-303 (1996) https://doi.org/10.1016/0192-0561(96)00028-8
  8. Wang, H.X., Ng, T. B., Liu, W.K, Ooi, V.E.C. and Chang, S.T. Polysaccharide-peptide complexes from the cultured mycelia of the mushroom Coriolus versicolor and their culture medium activate mouse lymphocytes and macrophages. Int. J. Biochem. Cell BioI. 28: :601-607 (1996) https://doi.org/10.1016/1357-2725(95)00157-3
  9. Collins, R.A. and Ng, T.B. Polysaccharopeptide from Coriolus versicolor has potential for use against human immunodeficiency virus type I infection. Life Sci. 60: 383-387 (1977) https://doi.org/10.1016/S0024-3205(96)00662-5
  10. Cheung, L.M., Cheung, P.C.K and Ooi, V.E.C. Antioxidant activity and total phenolics of edible mushroom extracts. Food Chem. 80: 1-7 (2003) https://doi.org/10.1016/S0308-8146(02)00227-3
  11. Mau, J.L., Lin, H.C., and Song, S.F. Antioxidant properties of several speciality mushrooms. Food Res. Int. 35: 519-526 (2002) https://doi.org/10.1016/S0963-9969(01)00150-8
  12. Yang, J.H., Lin, H.C. and Mau, J.L. Antioxidant properties of several commercial mushroom. Food Chem. 77: 229-235 (2002) https://doi.org/10.1016/S0308-8146(01)00342-9
  13. Liu, F., Ooi, V.E.C. and Chang, S.T. Free radical scavenging activities of mushroom polysaccharide extracts. Life Sci. 60: 763-771 (1997) https://doi.org/10.1016/S0024-3205(97)00004-0
  14. Lee, B.C., Bae, J.T, Pyo, H.B., Choe, T.B., Kim, S.W, Hwang, H.J. and Yun, J.W Biological activities of the polysaccharides produced from submerged culture of the edible Basidiomycete Grifola frondosa. Enz. Microb. Technol. 6274: 1-8 (2003)
  15. Choi, H.S., Cho, H.Y, Yang, H.C., Ra, K.S. and Suh, H.J. Angiotensin I-converting enzyme inhibitor from Grifola frondosa. Food Res. Int. 34: 177-182 (2001) https://doi.org/10.1016/S0963-9969(00)00149-6
  16. Kato, H., Lee, l.E., Chuyen, N.V., Kim, S.B. and Hayase, F. Inhibition of nitrosamine formation by nondialyzable melanoidins. Agric. BioI. Chem. 51: 1333-1338 (1987) https://doi.org/10.1271/bbb1961.51.1333
  17. Chu, Y.H., Chang, C.L, and Hsu, H.F. Flavonoid content of several vegetables and their antioxidant mushrooms (Agricus bisporus). J. Sci. Food Agric. 80: 561-566 (2000) https://doi.org/10.1002/(SICI)1097-0010(200004)80:5<561::AID-JSFA574>3.0.CO;2-#
  18. Kiharu, I., Miho, I. and Toshimi, H. Major antioxidative substances in leaves of atsumi-kabu. Agric. BioI. Chem. 54: 1053-1055 (1990) https://doi.org/10.1271/bbb1961.54.1053
  19. Beaucham, C. and Fridovich, I. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44: 276-287 (1971) https://doi.org/10.1016/0003-2697(71)90370-8
  20. Cushman, D.W. and Cheung, H.S. Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung. Biochem. Pharmacol. 20: 1637-1648 (1971) https://doi.org/10.1016/0006-2952(71)90292-9
  21. Carmichael, J.W.G., DeGraff, A., Gazdar, J.M. and Mitchell, J.B. Evaluation of a tetrazolium-based semiautomated colorimetric assay. Cancer Res. 47: 936-942 (1987)
  22. Maron, D.M. and Ames, B.N. Revised methods for the Salmonella mutagenicity test. Mutation Res. 113: 173-215 (1983) https://doi.org/10.1016/0165-1161(83)90010-9
  23. Kim, S.M., Cho, Y.S. and Sung, S.K. The antioxidant ability and nitrite scavenging ability of plant extracts. Korean J. Food Sci. Technol. 33: 626-632 (2001)
  24. Gray, J.I. and Dugan, J.R. Inhibition of N-nitrosamine in model food systems. J. Food Sci. 40: 981-984 (1975) https://doi.org/10.1111/j.1365-2621.1975.tb02248.x
  25. Kang, Y.H., Park, Y.K. and Lee, G.D. The nitrite scavenging and electron donating ability of phenolic compounds. Korean J. Food Sci. Technol. 28: 232-239 (1996)
  26. Park, S.W., Yu, K.H. and Min, T.J. Antioxidant activities of extracts from fruiting bodies of mushrooms. Korean J. Mycol. 26: 69-77 (1998)
  27. Kim, J.H. and Park, K.M. Nitrite scavenging and superoxide dismutase like activities of herbs, spices and curries. Korean J. Food Sci. Technol. 32: 706-712 (2000)
  28. Yeun, D.M., Lee, T.G., Byun, H.S., Kim, S.B., and Park, Y.H. Angiotensin-I converting enzyme inhibitory activity of enzymatic hydrolysates of mackerel muscle protein. Bull. Korean Fish. Soc. 23: 229-235 (1992)
  29. Chung, M.S., Jung, S.H., Lee, J.S. and Park, K.M. Physiological activities of commercial instant curry powders and individual spices. Korean J. Food Sci. Technol. 35: 125-131 (2003)
  30. Bok, J.W., Lee, S.K. and Kim, B.K. Studies of development of new pharmacologically active components of Ganoderma Lucidum. Korean Biochem. J. 27: 149-153 (1994)
  31. Bae, J.T. and Lee, K.R. Antimutagenic and DNA topoisomerase I inhibition effects of Sarcodon aspratus extracts. J. Korean Soc. Food Sci. Nutr. 29: 917-921 (2000)
  32. Kim, J.M. and Jung, Y.M. The mutagenicity of Ramaria botrytis extract. Korea J. Vet. Publ. Helth. 19: 191-197 (1995)