Effects of Fermentation Conditions on Production of Erythritol by Candida magnoliae

Candida magnoliae의 발효 조건이 erythritol의 생산에 미치는 영향

  • Published : 2003.08.01


This study was carried out to examine the effects of fermentation conditions on the production of erythritol by osmophilic yeast Candida magnoliae. It was found that sucrose was superior to glucose as carbon source and 109 g/L erythritol was produced from 400 g/L sucrose. When yeast extract was used as nitrogen source, maximum values of yield and productivity for erythritol were obtained at 15 and 20 g/L of yeast extract, respectively. A mixture of 15 g/L yeast extract and 3 g/L ammonium phosphate allowed more efficient utilization of sucrose and hence resulted in 149 g/L of erythritol, 0.37 g erythritol/g sucrose of erythritol yield and $0.78\;g/L{\cdot}hr$ of erythritol productivity. A batch fermentation supplemented with 40 g/L KCl resulted in an erythritol concentration of 167 g/L and an erythritol yield of 0.42 g erythritol/g sucrose.


erythritol;Candida magnoliae


  1. Park, J.B., Sco, B.C., Kim, J.R., Pek, U.H. and Park, Y.K. Effects of glucose concentration on the production of erythritol by Trichosporon sp. J. Microbiol. Biotechnol. 8: 543-546 (1998)
  2. Ishizuka, H., Wako, H., Kasumi, T. and Sasaki, T. Breeding of a mutant of Aureobasidium sp. with high erythritol production. J. Ferment. Bioeng. 68: 310-314 (1989) https://doi.org/10.1016/0922-338X(89)90003-2
  3. Kim, K.A., Noh, B.S., Lee, J.K., Kim, S.Y., Park, Y.C. and Oh, D.K. Optimization of culture conditions for erythritol production by Torula sp. J. Microbiol. Biotechnol. 10: 69-74 (2000)
  4. Koh, E.S., Moon, K.H., Han, K.C., Ryu, Y.W., and Seo, J.H. Optimization of culture conditions and nitrogen sources for production of erythritol by Candida magnoliae. Korean J. Appl. Microbiol. Biotechnol. 28: 349-354 (2000)
  5. Onishi, H. Production of polyalcohols by yeasts. Hakko Kyokaishi 25: 495-506 (1967)
  6. Lee, W.J. and Seo, J.H. Research status and prospect of fermented sweeteners. Bioindustry 8: 38 (1995)
  7. Goossen, J. and Roper, H. Erythritol, a new sweetener. Confect. Product 62:6-7 (1996)
  8. Oh, D.K., Cho, C.H., Lee, J.K. and Kim, S.Y. Increased erythritol production in fed-batch cultures of Torula sp. by controlling glucose concentration. J. Ind. Microbiol. Biotechnol. 26: 248-252 (2001) https://doi.org/10.1038/sj.jim.7000122
  9. Kim, S.Y., Park, S.S., Jeon, Y.J. and Seo, J.H. Analysis of fermentation characteristics for production of erythritol by Candida sp. Korean J. Food Sci. Technol. 28: 935-939 (1996)
  10. Kim, K.A., Noh, B.S., Kim, S.Y. and Oh, D.K. Effect of osmotic pressure of salts on growth of Torula sp. and erythritol production. Korean J. Appl. Microbiol. Biotechnol. 27: 91-95 (1999)
  11. Roper, H. and Goossens, J. Erythritol, a new raw material for food and on-food applications. Starch/Starke 45: 400-405 (1993) https://doi.org/10.1002/star.19930451107
  12. Kawanabe, J., Hirasawa, M., Takeuchi, T, Oda, T and Ikeda, T Non-cariogenicity of erythritol as a substrate. Caries Res. 26: 358-362 (1992) https://doi.org/10.1159/000261468
  13. Gancedo, J.M. and Lagunas, R. Contribution of the pentose-phos-phate pathway to glucose metabolism in Saccharomyces cerevisiae: A critical analysis on the use of labeled glucose. Plant Sci. Lett. 1: 193-200 (1973) https://doi.org/10.1016/0304-4211(73)90044-8