Effect of Whole Powder and Extracts of Gastrodiae Rhizoma on Serum Lipids and Body Fat in Rats Fed High-Fat Diet

천마 분말, 에탄올 및 열수추출물이 고지방식이를 급여한 흰쥐의 혈청지질과 체지방에 미치는 영향

  • Published : 2003.08.01


Effects of Gastrodiae Rhizoma on serum lipids and body fat in rats fed high-fat diet supplemented with 10% (w/w) lard during 11-week experimental period were investigated. Ninety-six male rats were randomly divided into eight groups: A, negative control (lard 10%); B, positive control (lard 10%+rat chow+5 brix water extract); C, lard 10%+1% G. Rhizoma powder; D, lard 10%+5% G. Rhizoma powder; E, lard 10%+2 brix 50% ethanol extract; F, lard 10%+10 brix 50% ethanol extract; G, lard 10%+2 brix water extract; H, lard 10%+10 brix water extract. Body weight gain, although slightly higher in groups A, B, and G, did not differ significantly among dietary groups. Weights of liver, spleen, kidney, and testis were significantly higher among dietary groups. Serum total cholesterol and triglyceride concentrations were markedly higher in both control groups than group H (p<0.05). Serum HDL concentration was significantly higher in group E, and lower in group A (p<0.05). Serum LDL+VLDL concentration was significantly lower in groups E, G, and H (p<0.05). Serum testosterone level was slightly higher in groups E, D, and H. Blood glucose level was significantly higher in groups B and G, whereas lower in group H (p<0.05). Epididymal fat pad (EFP) was markedly higher in control groups than G. Rhizoma diet-fed groups (p<0.05). The results indicated that decrease in serum lipids (TC, TG, LDL+VLDL) and body fat, and increase in serum HDL and testosterone levels were higher in groups E and H extracts than groups fed G. Rhizoma powder.


  1. Society of Oriental Medicine. The Modern Oriental Medicine, pp. 446-447. Hakchang-Sa, Seoul, Korea (1993)
  2. Ku, B.H. Experimental studies on the pharmaceutical effects of Gastrodiae elata. M.S. thesis, Kyung-Hee Univ., Seoul, Korea (1991)
  3. Kim, E.J., Ji, G.E. and Kang, Y.H. Effects of Gastrodia Rhizoma extracts on global coronary circulation in rats. Korean J. Food Sci. Technol. 26: 213-220 (1994)
  4. Reeves, P.G., Nielsen, F.H. and Fahey, G.C. AIN-93 purified diets for laboratory rodents. J. Nutr. 123: 1939-1951 (1993) https://doi.org/10.1093/jn/123.11.1939
  5. William, R.B., Robert, E. and Kathleen, N. Single-nutrient effects on immunologic functions. J. Am. Med. Assoc. 245: 53-58 (1981) https://doi.org/10.1001/jama.245.1.53
  6. Mertin, J., Meade, C.J. and Hunt, R. Importance of the spleen for the immuno-inhibitory action of linoleic acid in mice. Int. Archs. Allergy Appl. Immunol. 53: 469-473 (1977) https://doi.org/10.1159/000231785
  7. Green, M.S., Heiss, G., Rifkind, B.M., Cooper, G.R., Williams, O.D. and Tayroler, H.A. The ratio plasma high density lipoprotein cholesterol to total and low density lipoprotein cholesterol-age related changes and race and sex differences in selected North American populations. Circulation 72: 93-104 (1985) https://doi.org/10.1161/01.CIR.72.1.93
  8. Grundy, S.M. and Denky, M.A. Dietary influences on serum lipids and lipoproteins. J. Lipid Res. 31: 1149-1172 (1990)
  9. Wu, H.Q., Xie, L., Jin, X.N., Ge, Q., Jin, H. and Liu, G.Q. The effect of vanillin on the fully amygdala-kindled seizures in the rat. Yao-Hsueh-Hsueh-Pao 24: 482-489 (1989)
  10. Ebenbichler, C.F., Kichmair, R., Eagger, C. and Patch, J.R. Postprandial stage and atherosclerosis. Curr. Opinion Lipidol. 6: 286-290 (1995) https://doi.org/10.1097/00041433-199510000-00008
  11. Chung, H.S. and Ji, G.E. Composition and functionality of Chonma. Korean J. Food Sci. Technol. 26: 213-220 (1994)
  12. Lusis, A.J. Genetic factors affecting blood lipoprotein. J. Lipid Res. 29: 397-429 (1988)
  13. Friedewald, W.T., Levy, R.1. and Fredrickson, D.S. Estimation of LDL-cholesterol on plasma without use of the preparation ultracentrifuge. Clin. Chem. 18: 499-502 (1972)
  14. Bae, J.C. Development of functional foods using chunma. Food Ind. Nutr. 7: 50-54 (2002)
  15. Snedecor, G.W. and Cochrane, W.G. Statistical Methods. 6th ed., p. 1. Iowa State University Press, Iowa, USA (1967)
  16. Oh, S.S. and Kaplan, M.L. Early treatment of obese(ob/ob) mice with triiodothyronine increases oxidative metabolism in muscle but not in brown adipose tissue or liver. J. Nutr. 125: 112-124 (1995)
  17. Huang, J.H. Comparison studies on pharmacological properties of injection Gastrodia elata, gastrodin-free fraction and gastrodin. Chung-Kuo-Hsueh-Ko-Hsueh-Yuan-Hsueh-Pao 11: 147-152 (1989)
  18. Huang, Z.L. Recent developments in pharmacological study and clinical application of Gastrodia elata in China. Chung-Hsi-I-Chieh- Ho-Tsa-Chih 5: 251-258 (1985)
  19. Paik, Y.S., Song, J.K., Yoon, C.H., Chung, K.S. and Yun-Choi, H.S. Anti-platelet and anti-thrombotic effects of Gastrodia elata. Korean J. Pharmacogn. 26: 385-389 (1995)
  20. Jeong, M.K., Kim, M.H., Kang, N.E. and Kim, W.K. Effects of resistant starch on gut functions and plasma lipid profiles in rats fed high fat diet. J. Korean Soc. Food Sci. Nutr. 31: 271-276 (2002) https://doi.org/10.3746/jkfn.2002.31.2.271
  21. Goldberg, I.J. Lipoprotein lipase and lipolysis: Central roles in lipoprotein metabolism and artherogenesis. J. Lipid Res. 37: 693-707 (1996)