Isolation and Identification of Antioxidative Components from Bark of Rhus javanica Linne

붉나무 수피로부터 항산화 물질의 분리 및 동정

  • Oh, Ji-Young (Kimchi Research Center, Food Business Group, Doosan Co.) ;
  • Choi, Ung (Department of Hotel Culinary Arts and Nutrition, Sohae College) ;
  • Kim, Yong-Suk (Research Center for Industrial Development of Biofood materials, Chonbuk National University) ;
  • Shin, Dong-Hwa (Faculty of Biotechnology (Food Science and Technology), Chonbuk National University)
  • 오지영 ((주)두산 식품 BG/김치연구소) ;
  • 최웅 (서해대학교) ;
  • 김용석 (전북대학교 바이오식품 소재 개발 및 산업화 연구센터) ;
  • 신동화 (전북대학교 응용생물공학부 및 농업과학기술연구소)
  • Published : 2003.08.01

Abstract

The crude extracts from Rhus javanica Linne showed comparatively strong antioxidative activity in test oils. Antioxidative components were isolated and identified by column chromatography, thin layer chromatography, UV, and NMR. These antioxidative components were added to several oils to compare antioxidative activity with several commercial antioxidants, such as BHA, BHT, and tocopherol. After the sixth column chromatography, one fraction (R-18-9-3-2-4-2) was separated from chloroform layer of Rhus javanica Linne. The R-18-9-3-2-4-2 fraction was identified as methyl gallate by $^1H-NMR$ and $^{13}C-NMR$ and confirmed with methyl gallate standard as authentic. The R18-9-3-2-4-2 fraction from chloroform layer of Rhus javanica Linne showed stronger activity than that of the ${\alpha}-,\;{\delta}-tocopherol$, BHT, and BHA at the same concentration.

Keywords

Rhus javanica Linne;antioxidant;natural antioxidant;methyl gallate

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