Safety Study on General Cell Cytotoxicity Effects of Cervi Pantotrichum Cornu Herbal-acupuncture Solution(CPCHA)

녹용약침의 일반 세포 독성 시험에 의한 안전성 연구

  • 변부형 (대구한의대학교 한의과대학) ;
  • 서부일 (대구한의대학교 한의과대학)
  • Received : 0
  • Accepted : 0
  • Published : 0

Abstract

Objectives: The purpose of this study is to investigate general cell cytotoxicities effects of Cerui Pantotrichurn Cornu Herbal-acupuncture Solution(CPCHA). Methods: In this study we have been carried out to analize the effects of Cervi Pantotrichum Cornu Herbal-acupuncture Solution(1-10 ug/ml) on cytotoxicities in primary culture of adult rat hepatocytes using MTT method, LDH enzyme release, membrane transport of ouabain. Results: The results were obtained a s follows: Hepatotoxicities was not affected by treatment with Cerui Pantotrichurn Cornu Herbal-acupuncture Solution(CPCHA) of 5 ug/ml and 10 ug/ml . In primary culture of adult rat hepatocytes, the treatment of Cerui Pantotrichum Cornu Herbal-acupuncture Solution(CPCHA) did not affect LDH enzyme activity and membrane transport ouabain. In all parameters used in the present studies, CPCHA did not affect these normal functional characteristics. Conclusion: From the above results, it is suggested that Cerui Pantotrichum Cornu Herbal-acupuncture Solution(CPCHA) did not affect normal functional characteristics.

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