Use of Lycopene, an Antioxidant Carotinoid, in Laying Hens for Egg Yolk Pigmentation

  • Kang, D.-K. (Bio-Resources Institute, EASY BIO System, Inc.) ;
  • Kim, S.-I. (Bio-Resources Institute, EASY BIO System, Inc.) ;
  • Cho, C.-H. (Bio-Resources Institute, EASY BIO System, Inc.) ;
  • Yim, Y.-H. (Korea Research Institute of Standards and Science) ;
  • Kim, H.-S. (Bio-Resources Institute, EASY BIO System, Inc.)
  • Received : 2003.05.29
  • Accepted : 2003.08.20
  • Published : 2003.12.01


The possibility of lycopene affecting egg yolk pigmentation was studied with lycopene diets containing 0, 4, 8, and $12{\mu}g/g$ meal, respectively. The addition of lycopene above $4{\mu}g/g$ meal significantly improved yolk color after four days of supplementation. The transfer of lycopene into egg yolk was confirmed by thin layer chromatography, and high-performance liquid chromatography-mass spectrometry (HPLC-MS). The deposition rate of lycopene into egg yolk was approximately 2%, which was quantitatively determined using a HPLC with a UV detector. The result indicates that lycopene is a good candidate for egg yolk pigmentation and for making functional eggs.


Lycopene;HPLC-MS;Egg Yolk;Pigmentation;Deposition Rate


  1. Balnave, D. and J. N. Bird. 1996. Relative efficiencies of yellow carotinoids for egg yolk pigmentation. Asian-Aust. J. Anim. Sci. 9(5):515-517.
  2. Damron, B. L., S. R. Goodson, R. H. Harms, D. M. Janky and H. R. Wilson. 1984. Beta-carotene supplementation of laying hen diets. Br. Poult. Sci. 25(3):349-352.
  3. Erdman, J. W. Jr., G. C. Fahey Jr. and C. B. White. 1986. Effects of purified dietary fiber sources on $\beta$-carotene utilization by the chick. J. Nutr. 116:2415-2423.
  4. Gerster, H. 1997. The potential role of lycopene for human health. J. Am. Coll. Nutr. 16:109-126.
  5. Levy, J., E. Bosin, B. Feldman, Y. Giat, A. Miinster, M. Danilenko and Y. Sharoni. 1995. Lycopene is a more potent inhibitor of human cancer cell proliferation than either $\alpha$-carotene or $\beta$-carotene. Nutr. Cancer. 24:257-266.
  6. Tzouganaki, Z. D., J. Atta-Politou and M. A. Koupparis. 2002. Development and validation of liquid chromatographic method for the determination of lycopene in plasma. Anal. Chim. Acta. 467:115-123.
  7. DiMascio, P., S. Kaiser and H. Sies. 1989. Lycopene as the most efficient biological carotenoid singlet oxygen quencher. Arch. Biochem. Biophys. 274:532-538.
  8. Sikder, A. C., S. D. Chowdhury, M. H. Rashid, A. K. Sarker and S. C. Das. 1998. Use of dried carrot meal (DCM) in laying hen diet for egg yolk pigmentation. Asian-Aust. J. Anim. Sci. 11(3):239-244.
  9. Franceschi, S., E. Bidoli, C. LaVeccia, R. Talamini, B. D’Avanzo and E. Negri. 1994. Tomatoes and risk of digestive tract cancers. Int. J. Cancer. 59:181-184.
  10. Halliwell, B. 1994. Free radicals and antioxidants: a personal view. Nutr. Rev. 52:253-265.
  11. Stahl, W. and H. Sies. 1996. Lycopene; a biologically important carotinoid for human? Arch Biochem. Biophys. 336:1-9.
  12. Boileau, T. W., A. C. Boileau and J. W. Erdman, Jr. 2002. Bioavailability of all-trans and cis-isomers of lycopene. Exp. Biol. Med. 227:914-919.
  13. Helzlsouer, K. J., G. W. Comstock and J. S. Morris. 1989. Selenium, lycopene, $\alpha$-tocopherol, $\beta$-carotene, retinol and subsequent bladder cancer. Cancer Res. 49:6144-6148.
  14. Giovannucci, E., A. Ascherio. E. B. Rimm, M. J. Stampfer, G. A. Colditz and W. C. Willett. 1995. Intake of carotenoids and retinol in relation to risk of prostate cancer. J. Natl. Cancer Inst. 87:1767-1776.
  15. Hencken, H. 1992. Chemical and physiological behavior of feed carotinoids and their effects on pigmentation. Poult. Sci. 71:711-717.
  16. Pastori, M., H. Pfander, D. Boscoboinik and A. Azzi. 1998. Lycopene in association with alpha-tocopherol inhibits at physiological concentrations proliferation of prostate carcinoma cells. Biochem. Biophys. Res. Comm. 250:582-585.
  17. SAS. 1998. SAS User's Guide: Statistics. Statistical Analysis System Institute, Inc., Cary, NC.
  18. Janky, D. M., M. G. Dukes and R. H. Harms. 1981. Effect of 3-nitro-10 on egg yolk pigmentation. Poult. Sci. 61:573-574.
  19. Ollilainen, V., M. Heinonen, E. Linkola, P. Varo and P. Koivistoinen. 1989. Carotinoids and retinoids in Finnish Foods: Dairy Products and eggs. J. Dairy Sci. 72:2257-2265.
  20. Bohm, F., J. H. Tinkler and T. G. Truscott. 1995. Carotenoids protect against cell membrane damage by the nitrogen dioxide radical. Nat. Med. 2:98-99.
  21. Schaeffer, J., J. K. Tyczkowski, C. R. Parkhurst and P. B. Hamilton. 1987. Carotinoid composition of serum and egg yolks of hens fed diets varying in carotinoids composition. J. Poult. Sci. 67:608-614.
  22. Park, Y. H., H. K. Kim, H. S. Kim, H. S. Lee, I. S. Shin and K. Y. Whang. 2002. Effects of three different soybean meal source on layer and broiler performance. Asian-Aust. J. Anim. Sci. 15(2):254-265.
  23. Craft, N. E., S. A. Wise and J. H. Jr. Soares. 1992. Optimization of an isocratic high-performance liquid chromatographic separation of carotenoids. J. Chromatogr. 589:171-176.

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