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pH Affects the In vitro Formation of cis-9, trans-11 CLA and trans-11 Octadecenoic Acid by Ruminal Bacteria When Incubated with Oilseeds

  • Wang, J.H. (Department of Animal Science, Chungbuk National University) ;
  • Song, M.K. (Department of Animal Science, Chungbuk National University)
  • Received : 2002.03.04
  • Accepted : 2003.07.10
  • Published : 2003.12.01

Abstract

The effect of pH on the fermentation characteristics and the formation of cis-9, trans-11 conjugated linoleic acid (CLA) and trans-11 octadecenoic acid by mixed ruminal bacteria was examined in vitro when incubated with linseed or rapeseed. Concentrate (1%, w/v) with ground linseed (0.6%, w/v) or rapeseed (0.5%, w/v) was added to 600 ml mixed solution of strained rumen fluid with artificial saliva (1:1, v/v), and was incubated anaerobically for 12 h at $39^{\circ}C$. The pH of culture solution was maintained at level close to 4.5, 5.3, 6.1 and 6.9 with 30% $H_2SO_4$ or 30% NaOH solution. pH increment resulted in increases of ammonia and total volatile fatty acid (VFA) concentration in culture solutions containing both oilseeds. Fermentation did not proceeded at pH 4.5. Molar proportion of acetate decreased but that of propionate increased as pH increased when incubated with oilseeds. While the hydrogenating process was very slow at the pH range of 4.5 to 5.3, rapid hydrogenation was found from the culture solutions of pH 6.1 and 6.9 when incubated with linseed or rapeseed. As pH in culture solution of linseed or rapeseed increases proportions of oleic acid (cis-9 $C_{18:1}$) and trans-11 octadecenoic acid increased but those of linoleic acid and linolenic acid decreased. The CLA proportion increased with pH in culture solution containing rapeseed but CLA was mostly not detected from the incubation of linseed.

Keywords

pH;Oilseed;Biohydrogenation;cis-9;trans-11 CLA;trans-11 Octadecenoic Acid;In vitro;Mixed Ruminal Bacteria

References

  1. Chin, S. F., W. Liu, J. M. Storkson, Y. L. Ha and M. W. Pariza. 1992. Dietary sources of conjugated dienoic isomers of linoleic acid, a newly recognized class of anticarcinogens. J. Food Compos. Anal. 5:185-197.
  2. Kepler, C. R., W. P. Tucker and S. B. Tove. 1970. Biohydrogenation of unsaturated fatty acids. Ⅳ. Substrate specificity and inhibition of linoleate $\Delta$12-cis, $\Delta$11-trans isomerase from Butyrivibrio fibrisolvens. J. Biol. Chem. 245:3612-3620.
  3. Latham, M. J., J. E. Storry and M. E. Sharpe. 1972. Effect of low roughage diets on the microflora and lipid metabolism in the rumen. Appl. Microbiol. 24:871-877.
  4. Romo, G. A. 1995. Trans fatty acids: rumen in vitro production and their subsequent metabolic effects on energy metabolism and endocrine responses in the lactating dairy cow. Ph.D. Diss. Univ. Maryland, College Park.
  5. SAS. 1985. SAS User′s Guide: Statistical Analysis Systems Institute, Inc., Cary, NC.
  6. Van Nevel, C. J. and D. I. Demeyer. 1996. Influence of pH on lipolysis and biohydrogenation of soybean oil by rumen contents in vitro. Reprod Nutr. Dev. 36:53-63.
  7. Ha, Y. L., N. K. Grimm and M. W. Pariza. 1987. Anticarcinogens from fried ground beef: heat-altered derivatives of linoleic acid. Carcinogenesis 8:1881-1887.
  8. Harfoot, C. G. and G. P. Hazlewood. 1988. Lipid metabolism in the rumen. Page 285. In: The rumen microbial ecosystem. P.N.Hobson, ed. Elsevier Appl. Sci., London and NY.
  9. Sehat, N, M. P. Yurawecz, J. A. G. Roach, M. M. Mossoba, J. K. G. Kramer and Y. Ku. 1998. Silver-ion high-performance liquid chromatographic separation and identification of conjugated linoleic acid isomers. Lipid. 33:217-221.
  10. Chouinard, P. Y., L. Corneau, D. E. Bauman, W. R. Butler, Y. Chilliard and J. K. Drackley. 1998. Conjugated linoleic acid content of milk from cows fed different sources of dietary fat. J. Anim. Sci. Vol. 76, suppl. 1/J. Dairy Sci. Vol. 81, suppl. 1 (Abstract).
  11. Hughes, P. E., W. J. Hunter and S. B. Tove. 1982. Biohydrogenation of unsaturated fatty acids. Purification and properties of cis-9, trans-11-octadecadienoate reductase. J. Biol. Chem. 257:3643-3649.
  12. Stewart, C. S. 1977. Factors affecting the cellulolytic activity of rumen contents. Appl. Environ. Microbiol. 33:497-501.
  13. Dhiman, T. R., G. R. Anand, L. D. Satter and M. W. Pariza. 1999. Conjugated Linoleic Acid Content of Milk from Cows Fed Different Diets. J. Dairy Sci. 82:2146-2156.
  14. McDougall, E. I. 1948. Studies on ruminant saliva. 1. The composition and output of sheeps saliva. Biochem. J. 43:99-109.
  15. Wahle, K. W. and W. P. James. 1993. Isomeric fatty acids and human health. Eur. J. Clin. Nutr. 47:828-839.
  16. Folch, J., M. Lee and G. H. Sloan-Stanley. 1957. A simple method for the isolation and purification of total lipids from animal tissue. J. Biol. Chem. 226:497-509.
  17. Wang, J. H. and M. K. Song. 2001. Effect of sources and levels of carbohydrates on fermentation characteristics and hydrogenation of linoleic acid by rumen bacteria in vitro. Asian-Aust. J. Anim. Sci. 14:48-53.
  18. Kalscheur, K. F., B. B. Teter, L. S. Piperova and R. A. Erdman. 1997. Effect of dietary forage concentration and buffer addition on duodenal flow of trans-$C_{18:1}$ fatty acids and milk fat production in dairy cows. J. Dairy Sci. 80:2104-2114.
  19. Kopecny, J. and R. J. Wallace. 1982. Cellular location and some properties of proteolytic enzymes of rumen bacteria. Appl. Environ. Microbiol. 43:1026-1033.
  20. Wang, J. H. and M. K. Song. 1999. Effect of dextrose addition levels on hydrogenation and incorporation of oleic acid by ruminal bacteria. Kor. J. Anim. Nutr. Feed. 23:335-344.
  21. Fawcett, J. K. and J. E. Scott. 1960. A rapid and precise method for the determination of urea. J. Clin. Pathol. 13:156-163.
  22. Lepage, G. and C. C. Roy. 1986. Direct transesterification of all classes of lipid in a one-step reaction. J. Lipid Research. 27:114-121.
  23. Kemp, P. and D. J. Lander. 1984. Hydrogenation in vitro of alphalinoleic acid to stearic acid by mixed cultures of pure strains of rumen bacteria. J. Gen. Microbial. 130:527-533.
  24. Steel, R. G. D. and J. H. Torrie. 1980. Principles and Procedures of Statistics. Mcgraw Hill Book Co., NY.
  25. Ip, C., J. A. Scimeca and H. Thompson. 1995. Effect of timing and duration of dietary conjugated linoleic acid on mammary cancer prevention. Nutr. Cancer 24:241-247.
  26. Kelly, M. L., J. R. Berry, D. A. Dwyer, J. M. Griinari, P. Y. Chouinard, M. E. V. Amburgh and D. E. Bauman. 1998. Dietary fatty acid sources affect conjugated linoleic acid concentrations in milk from lactating dairy cows. J. Nutr. 128:881-885.
  27. Wang, J. H., M. K. Song, Y. S. Son and M. B. Chang. 2002a. Effect of concentrate level on the formation of conjugated linoleic acid and trans-octadecenoic acid by ruminal bacteria when incubated with oilseeds in vitro. Asian-Aust. J. Anim. Sci. 15:687-694.
  28. Wang, J. H., M. K. Song, Y. S. Son and M. B. Chang. 2002b. Addition effect of seed-associated or free linseed oil on the formation of cis-9, trans-11 conjugated linoleic acid and octadecenoic acid by ruminal bacteria in vitro. Asian-Aust. J. Anim. Sci. 15:1115-1120.
  29. Wang, J. H., S. H. Choi and M. K. Song. 2003. Effects of concentrate to roughage ratio on the formation of cis 9, tran 11-CLA and trans 11-Octadecenoic acid in rumen fluid and plasma of sheep when fed high oleic or high linoleic acid oils. Asian-Aust. J. Anim. Sci. 16(11):1604-1609.