- Volume 16 Issue 4
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Determination of Intestinal Disappearance of Lysine and Methionine Derived from Ruminally Protected Lysine and Methionine in Holstein Heifers
- Watanabe, K. (Ajinomoto Co.) ;
- Sato, H. (Ajinomoto Co., Inc.) ;
- Kobayashi, T. (Laboratory of Animal Physiology, Graduate School of Ajinomoto Co., Inc.) ;
- Katoh, K. (Laboratory of Animal Physiology, Graduate School of Agricultural Science, Tohoku University) ;
- Obara, Y. (Laboratory of Animal Physiology, Graduate School ofAgricultural Science, Tohoku University)
- Received : 2002.06.10
- Accepted : 2002.09.26
- Published : 2003.04.01
In order to estimate the availability of ruminally-protected Lys (RPLys) and Met (RPMet), quantitative changes of free Lys and Met in the total abomasal outflow and feces were determined in three Holstein heifers with a T-shaped duodenal cannula. RPLys was prepared by coating Lys with fat and RPMet with a pH-sensitive polymer. RPLys and RPMet containing 30 g of each amino acid were supplied together to heifers, and total abomasal outflow was collected hourly from the T-shaped duodenal cannula for a 48 h period. Collected abomasal outflows were fractionated into liquid and solid phases to measure free Lys and Met concentration. At 2 wk after total abomasal outflow sampling, heifers were supplied RPLys and RPMet together again, and total feces were collected every 12 h for a 3 d period t estimate excreted RPLys and RPMet in feces. The amounts of Lys and Met recovered from the liquid fractions of abomasal outflow were estimated to be
Ruminally Protected Lysine;Ruminally Protected Met;Heifer;Intestinal Disappearance
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