Determination of Intestinal Disappearance of Lysine and Methionine Derived from Ruminally Protected Lysine and Methionine in Holstein Heifers

  • Watanabe, K. (Ajinomoto Co.) ;
  • Sato, H. (Ajinomoto Co., Inc.) ;
  • Kobayashi, T. (Laboratory of Animal Physiology, Graduate School of Ajinomoto Co., Inc.) ;
  • Katoh, K. (Laboratory of Animal Physiology, Graduate School of Agricultural Science, Tohoku University) ;
  • Obara, Y. (Laboratory of Animal Physiology, Graduate School ofAgricultural Science, Tohoku University)
  • Received : 2002.06.10
  • Accepted : 2002.09.26
  • Published : 2003.04.01


In order to estimate the availability of ruminally-protected Lys (RPLys) and Met (RPMet), quantitative changes of free Lys and Met in the total abomasal outflow and feces were determined in three Holstein heifers with a T-shaped duodenal cannula. RPLys was prepared by coating Lys with fat and RPMet with a pH-sensitive polymer. RPLys and RPMet containing 30 g of each amino acid were supplied together to heifers, and total abomasal outflow was collected hourly from the T-shaped duodenal cannula for a 48 h period. Collected abomasal outflows were fractionated into liquid and solid phases to measure free Lys and Met concentration. At 2 wk after total abomasal outflow sampling, heifers were supplied RPLys and RPMet together again, and total feces were collected every 12 h for a 3 d period t estimate excreted RPLys and RPMet in feces. The amounts of Lys and Met recovered from the liquid fractions of abomasal outflow were estimated to be $23.9{\pm}8.3%$ and $68.6{\pm}3.6%$ (p=0.008) of RPLys and RPMet ingested, respectively. Final intestinal disappearances of Lys and Met were estimated to be $49.5{\pm}2.6%$ and $78.2%{\pm}6.5%$ (p=0.015) of ingested RPLys and RPMet, respectively.


Ruminally Protected Lysine;Ruminally Protected Met;Heifer;Intestinal Disappearance


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