- Volume 16 Issue 2
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Effects of Microbial Phytase Replacing Partial Inorganic Phosphorus Supplementation and Xylanase on the Growth Performance and Nutrient Digestibility in Broilers Fed Wheat-based Diets
- Peng, Y.L. (College of Animal Science and Technology, China Agricultural University) ;
- Guo, Y.M. (College of Animal Science and Technology, China Agricultural University) ;
- Yuan, J.M. (College of Animal Science and Technology, China Agricultural University)
- Received : 2002.07.02
- Accepted : 2002.09.12
- Published : 2003.02.01
Two experiments were conducted with broilers to investigate the feasibility of microbial phytase replacing partial inorganic phosphorus supplementation and the synergistic effects of xylanase (320 FTU/kg) supplementation alone or in combination with phytase (750 U/kg) replacing 0.08% dietary inorganic phosphorus, on the growth performance and utilization of nutrients in broilers fed wheat-based diets. In Experiment 1, 540 broilers were fed five diets for 6 weeks. Diets C0 and C1 were corn-based diets and 0.08% inorganic P supplementation was replaced with 750 U phytase/kg feed in Diet C1. Diets W0, W1 and W2 were wheat-based diets supplemented with microbial phytase 0, 750, 750 U/kg feed and 0, 0.08% and 0.16% dietary inorganic P were replaced, respectively. In Experiment 2, 432 broilers were divided into four treatments to determine the synergistic effects of supplemental xylanase and phytase replacing 0.08% inorganic P. Four experimental diets were arranged according to a
Broilers;Phytase;Xylanase;Wheat-based Diet;Inorganic Phosphorus
- Annison, G. 1990. Polysaccharide composition of Australian wheats and the digestibility of their starches in broiler chicken diets. Aust. J. Exp. Agric. 30:183-186. https://doi.org/10.1071/EA9900183
- Barrier-Guillot, B., P. Casado, P. Maupetit, C. Jondreville and F. Gatel. 1996. Wheat phosphorus availability: 2. In vivo study in broilers and pigs: relationship with endogenous phytasic activity and phytic phosphorus content in wheat. J. Sci. Food Agric. 70:69-74. https://doi.org/10.1002/(SICI)1097-0010(199601)70:1<69::AID-JSFA466>3.0.CO;2-C
- Crouch, A. N., J. L. Grimes, P. R. Ferker and L. N. Thomas. 1997. Enzyme supplementation to enhance wheat utilization in starter diets for broilers and turkeys. J. Appl. Poult. Res. 6:147-154. https://doi.org/10.1093/japr/6.2.147
- Fruhback, G., R. Alonso, F. Marzo and S. Santidrian. 1995. A modified method for the indirect quantitative analysis of phytate in foodstuffs. Anal. Biochem. 225:206-212. https://doi.org/10.1006/abio.1995.1145
- Jongbloed, A. W., P. A. Kemme, Z. Mroz and R. Jongbloed. 1996. The effects of organic acids in diets for growing pigs on the efficacy of microbial phytase. In: Phytase in Animal Nutrition and Waste Management (Ed. M. B. Coelho and E. T. Kornegay). BASF Corp., Mount Olive, NJ. pp. 515-524.
- Kornegay, E. T., D. M. Denbow and Z. Zhang. 1997. Phytase supplementation of corn-soybean meal broilers diets from three to seven week. Poult. Sci. 76(Suppl. 1):6(Abstr.).
- Larsson, M., M. Minekus and R. Havenaar. 1997. Estimation of the bioavailability of iron and phosphorus in cereals using a dynamic in vitro gastrointestinal model. J. Sci. Food Agric. 74:99-106. https://doi.org/10.1002/(SICI)1097-0010(199705)74:1<99::AID-JSFA775>3.0.CO;2-G
- Maisonnier, S., J. Gomez and B. Carre. 2001. Nutrient digestibility and intestinal viscosities in broiler chickens fed on wheat diets, as compared to maize diets with added guar gum. Br. Poult. Sci. 42:102-110. https://doi.org/10.1080/00071660020035154
- Marquardt, R. R., A. Brenes, Z. Zhang and D. Boros. 1996. The use of enzymes to improve nutrient availability in poultry feedstuffs. Anim. Feed Sci. Technol. 60:321-330. https://doi.org/10.1016/0377-8401(96)00987-X
- Mollah, Y., W. L. Bryden, I. R. Wallis, D. Balnave and E. F. Annison. 1983. Studies on low metabolizable energy wheats for poultry using conventional and rapid assay procedures and the effects of processing. Br. Poult. Sci. 24:81-89. https://doi.org/10.1080/00071668308416716
- Peter, C. M. and David H. Baker. 2001. Microbial phytase does not improve protein-amino acid utilization in soybean meal fed to young chicks. J. Nutr. 131:1792-1797.
- Rama Rao, S. V. and V. Ramasubba Reddy. 2001. Phytic phosphorus for eco-friendly products. Poult. International. 40:46-52.
- Ravindran, V., L. I. Hew, G. Ravindran, R. J. Gill, P. H. Pittolo and W. L. Bryden. 1999. Influence of xylanase supplementation on the apparent metabolisable energy and ileal amino acid digestibility in a diet containing wheat and oats, and on the performance of three strains of broiler chickens. Aust. J. Agric. Res. 50:1159-1163. https://doi.org/10.1071/AR98298
- Zhang, Z. B., E. T. Kornegay, J. S. Radcliffe, D. M. Denbow, H. P. Veit and C. T. Larsen. 2000. Comparison of genetically engineered microbial and plant phytase for young broilers. Poult. Sci. 79:709-717. https://doi.org/10.1093/ps/79.5.709
- NRC. 1994. Nutrient requirements of poultry. 9th edn. National Academy Press, Washington, DC.
- Fengler, A. I. and R. R. Marquardt. 1988. Water-soluble pentosans from rye: Effects of rate of dialysis on the retention of nutrients by the chick. Cereal Chemistry 65:298-302.
- McCance, R. A. and E. M. Widdowson. 1944. Activity of the phytase in different cereal and its resistance to heat. Nature. 153:660. https://doi.org/10.1038/153660a0
- Fuente, J. M., P. Perez De Ayala, A. Flores and M. J. Villamide. 1998. Effect of storage time on the metabolizable energy and digesta viscosity of barley-based diets for poultry. Poult. Sci. 77:90-97. https://doi.org/10.1093/ps/77.1.90
- Kiiskinen, T., J. Piironen and T. Hakonen. 1994. Effects of supplemental phytase on performance of broiler chickens. Agric. Sci. Fin. 3:457-466.
- Kornegay, E. T. 1999. A review of phosphorus digestion and excretion as influenced by microbial phytase in poultry. BASF Technical symposium, January 16, Atlanta, GA. BASF Corp., Mount Olive, NJ.
- Iji, P. A., R. J. Hughes, M. Choct and D. R. Tivey. 2001. Intestinal structure and function of broiler chickens on wheat-based diets supplemented with a microbial enzyme. Asian-Aust. J. Anim. Sci. 14:54-60.
- Ravindran, V. and W. L. Bryden. 1997. Influence of dietary phytic acid and available phosphorus levels on the response of broilers to supplemental Natuphos. Poultry Research Foundation of the University of Sydney. Report to BASF Australia Ltd., Sydney, Australia.
- Scott, T. A., F. G. Silversides, H. L. Classen, M. L. Swift and M. R. Bedford. 1999. Prediction of the performance of broiler chicks from apparent metabolizable energy and protein digestibility values obtained using a broiler chick bioassay. Can. J. Anim. Sci. 79:59-64. https://doi.org/10.4141/A98-054
- Schoner, F. J. 1993. Comparative effects of phytase and inorganic phosphorus in male chicken: the influence on performance data, mineral retention and dietary Ca. J. Anim. Physiol. Anim. Nutr. 69:235-244. https://doi.org/10.1111/j.1439-0396.1993.tb00810.x
- Zyla, Krzysztof, Dorota Gogol, Jerzy Koreleski, Sylwester Swiatkiewicz and David R. Ledoux. 1999b. Simultaneous application of phytase and xylanase to broiler feeds based on wheat: feeding experiment with growing broilers. J. Sci. Food Agric. 79:1841-1848. https://doi.org/10.1002/(SICI)1097-0010(199910)79:13<1841::AID-JSFA463>3.0.CO;2-G
- Coelho, M. B. and E. T. Kornegay. 1996. Phytase in animal nutrition and waste management. BASF Corporation, Mount Olive, NJ.
- Bolton, W. 1955. The digestibility of the carbohydrate complex by birds of different ages. J. Agric. Sci. 46:420. https://doi.org/10.1017/S002185960004048X
- Nelson, T. S. 1976. The hydrolysis of phytate phosphorus by chicks and laying hens. Poult. Sci. 55:1552-1562.
- AOAC. 1990. Official Methods of Analysis. 15th edn. Association of Official Analytical Chemists, Arlington, Virginia.
- Alko Biotechnology. 1990. Assay of phytase activity, method B-021. Rajamaki, Finland.
- Um, J. S., H. S. Lim, S. H. Ahn and I. K. Paik. 2000. Effects of microbial phytase supplementation to low phosphorus diets on the performance and utilization of nutrients in broiler chickens. Asian-Aus. J. Anim. Sci. 13:824-829.
- Rogel, A. M., E. F. Annison, W. L. Bryden and D. Balnave. 1987. The digestion of wheat starch in broiler chickens. Aust. J. Agric. Res. 38:639-649. https://doi.org/10.1071/AR9870639
Ouhida, J. F., Perez J. GASA and F. Puchal. 2000. Enzymes (
$\beta$-glucanase and arabinoxylanase) and/or sepiolite supplementation and the nutritive value of maize-barley-wheat based diets for broiler chickens. Br. Poult. Sci. 41:617-624. https://doi.org/10.1080/713654974
- Yinbo, Q., G. Peiji, W. Dong, Z. Xin and Z. Xiao. 1996. Production, Characterization, and application of cellulase-free xylanase from Aspergillus niger. Appl. Biochem. Biotechnol. 57/58: 375-381. https://doi.org/10.1007/BF02941716
- Bagheri, S. and L. Gueguen. 1985. Effect of wheat bran and pectin on the absorption and retention of phosphorus, calcium, magnesium and zinc by the growing pig. Rep. Nutr. Dev. 25:705-716. https://doi.org/10.1051/rnd:19850510
- Piao, X. S., In K. Han, J. H. Kim, W. T. Cho, Y. H. Kim and Chao Liang. 1999. Effects of kemzyme, phytase and yeast supplementation on the growth performance and pollution reduction of broiler chicks. Asian-Aus. J. Anim. Sci. 12:36-41. https://doi.org/10.5713/ajas.1999.36
- Simons, P. C. M. and H. A. J. Versteegh. 1990. Improvement of phosphorus availability by microbial phytase in broilers and pigs. Br. J. Nutr. 64:525-540. https://doi.org/10.1079/BJN19900052
- Jongbloed, A. W., Z. Mroz and P. A. Kemme. 1992. The effect of supplementary Aspergillus niger phytase in diets for pigs in concentration and apparent digestibility of dry matter, total phosphorus and phytic acid in different sections of the alimentary tract. J. Anim. Sci. 70:1155-1168.
- Eeckout, W. and M. De Paepe. 1994. Total phosphorus, phytatephosphorus and phytase activity in plant feedstuffs. Anim. Feed Sci. Technol. 47:19-29. https://doi.org/10.1016/0377-8401(94)90156-2
- Newirk, R. W. and H. L. Classen. 1996. The effects of dietary xylanase, phytase and phosphorus on the performance of laying hens. Poult. Sci. 72(Suppl 1):17(Abstr.).
- Annison, G. 1993. The role of wheat non-starch polysaccharide in broiler nutrition. Aust. J. Agric. Res. 44:405-422.
Almirall, M., M. Frencesch, A. M. Perez-Vendrell, J. Brufau and E. Esteve_Garcia. 1995. The differences in intestinal viscosity produced by barley and
$\beta$-glucanase alter digesta enzymes activities and ileal nutrient digestibilities more in broiler chicks than in cocks. J. Nutr. 125:947-955.
- Qian, H., H. P. Veit, E. T. kornegay, V. Ravindran and D. M. Denbow. 1996. Effects of supplemental phytase and phosphorus on histological and other tibial bone characteristics and performances of broilers fed semi-purified diets. Poult. Sci. 75:618-626. https://doi.org/10.3382/ps.0750618
- Zyla, Krzysztof, Dorota Gogol, Jerzy Koreleski, Sylwester Swiatkiewicz and David R Ledoux. 1999a. Simultaneous application of phytase and xylanase to broiler feeds based on wheat: in vitro measurements of phosphorus and pentose release from wheats and wheat-based feeds. J. Sci. Food Agric. 79:1832-1840. https://doi.org/10.1002/(SICI)1097-0010(199910)79:13<1832::AID-JSFA441>3.0.CO;2-Q
- Significance of phytic acid and supplemental phytase in chicken nutrition: a review vol.64, pp.04, 2008, https://doi.org/10.1017/S0043933908000202