Comparison of Natural Resistance-associated Macrophage Protein (NRAMP)1 Expression between Cows with High and Low Milk Somatic Cells Counts

  • Joo, Y.S. (National Veterinary Research and Quarantine Service) ;
  • Moon, J.S. (National Veterinary Research and Quarantine Service) ;
  • Fox, L.K. (College of Veterinary Medicine, Washington State University, Field Disease Investigation Unit) ;
  • Suh, G.H. (Veterinary Medicine Research Laboratory, Department of Livestock Improvement and Improvement Technology Research, National Livestock Institute, Rural Development Administration) ;
  • Kwon, N.H. (Department of Microbiology, College of Veterinary Medicine and School of Agriculture Biotechnology) ;
  • Kim, S.H. (Department of Microbiology, College of Veterinary Medicine and School of Agriculture Biotechnology) ;
  • Park, Y.H. (College of Veterinary Medicine, Washington State University, Field Disease Investigation Unit)
  • Received : 2002.02.12
  • Accepted : 2003.07.28
  • Published : 2003.12.01


Studies using natural resistance-associated macrophage protein (NRAMP) identification indicated that cattle could be selected for immunity. Several studies performed on intracellular organisms such as Mycobacterium, Salmonella, Brucella and Leishmania in human and mouse revealed that resistance against these bacteria was dependent on high activity of NRAMP1 in macrophages. However, hardly any researches have been done on Staphylococcus aureus in bovine mastitis, which is an intracellular organism and the main cause of bovine mastitis. The objectives of this study were to establish reverse transcriptase polymerase chain reaction (RT-PCR) methods, through which NRAMP1 mRNA expression could be compared and analyzed between mastitis-resistant and -susceptible cows. NRAMP1 gene and its expression were investigated using 20 cows (Holstein Friesian) in Korea. Cows were evenly split into two groups, with and without histories of clinical mastitis. Equivalent numbers of cows were randomly selected from each group. Monocytes were isolated from the bovine peripheral blood of each selected cows and activated with lipopolysaccharide (LPS). mRNA was separated from the monocytes and cDNA of NRAMP1 was synthesized and amplified using RT-PCR with amplification of $\beta$-actin as a control. The difference in NRAMP1 expressions of mastitis-resistant (n=10) and -susceptible (n=10) Holstein cows was analyzed. Results demonstrate that resistant cows produced more NRAMP1 mRNA than the susceptible ones, and ratios of NRAMP1:$\beta$-actin expression were higher in resistant cows with or without LPS activation. Therefore, this study could be applied to select bovine mastitis resistant cows before infection based on the expression of NRAMP1.


NRAMP1;RT-PCR;Bovine mastitis;Staphylococcus aureus


Supported by : Brain Korea 21


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