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Status of Antioxidant Enzymes in Normal Cycling and α-Tocopherol Supplemented Anestrus Buffalo Heifers (Bubalus bubalis)

  • Kahlon, R.S. (Department of Veterinary Physiology, College of Veterinary Science, Punjab Agricultural University) ;
  • Singh, Rajvir (Department of Veterinary Physiology, College of Veterinary Science, Punjab Agricultural University)
  • Received : 2002.06.18
  • Accepted : 2002.10.18
  • Published : 2003.02.01

Abstract

The present investigation was undertaken to study status of erythrocytic antioxidant enzymes in normal cycling and $\alpha$- tocopherol supplemented anestrus buffalo heifers. The pre-supplementation erythrocytic activities of superoxide dismutase (U/mg Hb), glutathione peroxidase (U/mg Hb) and glucose-6-phosphate dehydrogenase (U/g Hb) upregulated significantly (p<0.05) in anestrus heifers ($10.08{\pm}0.09$, $14.09{\pm}0.54$, $9.25{\pm}0.29$) when compared to normal cycling ones ($6.93{\pm}0.04$, $11.61{\pm}0.19$, $5.58{\pm}0.26$). The oral supplementation of $\alpha$-tocopherol (a) 3,000 mg per week per animal in anestrus heifers declined erythrocytic superoxide dismutase and glucose-6-phosphate dehydrogenase activities significantly (p<0.01) but led to non-significant increase in erythrocytic glutathione peroxidase activity. Results indicated that supplementation of $\alpha$-tocopherol to anestrus buffalo heifers mitigated the effects of oxidative stress to improve their antioxidant status.

Keywords

Antioxidant Enzyme;$\alpha$-Tocopherol;Anestrus;Buffalo Heifers

References

  1. Chow, C. K., K. Reddy and A. L. Tappel 1973. Effect of dietary vitamin E on the activities of the glutathione peroxidase system in rat tissue. J. Nutr. 103:618-624.
  2. Ferrell, P. M. and R. J. Robert. 1994. Vitamin E. In: Modern Nutrition in Health and Disease (Ed. M. E. Shils, J. A. Olson and M. Shike). 8th ed, Lea & Febiger, Philadelphia. pp. 326-341.
  3. Mehlert, A. and A. T. Diplock. 1985. The glutathione-S-transferase in selenium and vitamin E deficiency. Biochem. J. 227:823-831. https://doi.org/10.1042/bj2270823
  4. Walsh, D. S., D. G. Kennedy, E. A. Goodall and S. Kennedy 1993b. Antioxidant enzyme activity in muscle of calves depleted of vitamin E or selenium or both. Br. J. Nutr. 70:621-630. https://doi.org/10.1079/BJN19930153
  5. Lawrence, R. A., L. K. Parkhill and R. F. Burk. 1978. Hepatic cytosolic non selenium - dependent glutathione peroxidase activity : its nature and the effect of selenium deficiency. J. Nutr. 108:981-987.
  6. Rock, C. L., R. A. Jacob and P. E. Bowen. 1996. Update on the biological characteristics of the antioxidant micronutrients : vitamin C, vitamin E and the carotenoids. J. Am Diet. Assoc. 96:693-702. https://doi.org/10.1016/S0002-8223(96)00190-3
  7. Hafeman, D. G., R. A. Sunde and W. G. Hoekstra. 1974. Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rat. J. Nutr. 104:580-587.
  8. Putnam, M. E. and N. Comben. 1987. Vitamin E. Vet. Rec. 121:541-545.
  9. Esterbauer, H., R. J. Schaur and H. Zollner. 1991. Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free Rad. Biol. Med. 11:81-128. https://doi.org/10.1016/0891-5849(91)90192-6
  10. Nishikimi, M., N. A. Rao and K. Yagi. 1972. The occurrence of superoxide anion in the reaction of reduced phenazine methosufate and molecular oxygen. Biochem. Biophys. Res. Commun. 46:849-854. https://doi.org/10.1016/S0006-291X(72)80218-3
  11. Rotruck, J. T., A. L. Pope, H. E., Ganther, A. B. Swanson, D. G. Hafeman and W. G. Hoekstra. 1973. Selenium : biochemical role as a component of glutathione peroxidase. Science 179: 588-590. https://doi.org/10.1126/science.179.4073.588
  12. Deutsch, J. 1978. Maleimide as an inhibitor in measurement of erythrocyte glucose-6-phosphate dehydrogenase activity. Clin. Chem. 24:885-889.
  13. Zoeren-Grobben, D. van, J. H. N. Lindeman, E. Houdkamp, R. Bland, J. Schrijver, H. M. Berger and D. Van-Zoeren-Grobben. 1994. Postnatal changes in plasma chain breaking antioxidants in healthy pre term infants fed formula and/or human milk. Am. J. Clin. Nutr. 60:900-906. https://doi.org/10.1093/ajcn/60.6.900
  14. Jukola, E., J. Hakkarainen, H. Saloniemi and S. Sankari. 1996. Blood selenium, vitamin E, vitamin A and $\beta$-carotene concentrations and udder development, fertility treatments and fertility. J. Dairy Sci. 79:838-845. https://doi.org/10.3168/jds.S0022-0302(96)76432-9
  15. Burton, G. W. and M. G. Traber 1990. Vitamin E : antioxidant activity, biokinetics and bioavailability. Annu. Rev. Nutr 10:357-382. https://doi.org/10.1146/annurev.nu.10.070190.002041
  16. Harvey, J. W. 1989. Erythrocyte metabolism In: Clinical Biochemistry of Domestic Animals. (Ed. J. J. Kaneko). 4th ed, Academic Press, New York. pp. 185-234.
  17. Kayden, H. J., C. K. Chow and L. K. Bjornson. 1973. Spectrophotometric method for determination of tocopherol in red blood cells. J. Lipid Res. 14:533-540.
  18. Kahlon, R. S. 1999. Studies on antioxidant status of normal cycling, delayed pubertal and anesrus buffaloes (Bubalus bubalis). Ph.D. dissertation, Punjab Agricultural University, Ludhiana, India.
  19. Alin, P., U. H. Danielson and B. Mannervik. 1985. 4-Hydroxyalk- 2-enals are substances for glutathione transferase. FEBS Letters 179:269-270.
  20. Walsh, D. M., S. Kennedy, W. J. Blanchflower, E. A. Goodall and D. G. Kennedy. 1993a. Vitamin E and selenium deficiencies increase indices of lipid peroxidation in ruminant calves. Int. J. Vit. Nutr. Res. 63:188-194.
  21. Mayes, P. A. 1996. Structure and function of lipid soluble vitamins. In: Harper's Biochemistry (Ed. R. K. Murray, D. K. Granner, P. A. Mayes and V. M. Rodwell). 24th ed, Prentice Hall International limited, London. pp. 614-624.