Status of Antioxidant Enzymes in Normal Cycling and α-Tocopherol Supplemented Anestrus Buffalo Heifers (Bubalus bubalis)

  • Kahlon, R.S. (Department of Veterinary Physiology, College of Veterinary Science, Punjab Agricultural University) ;
  • Singh, Rajvir (Department of Veterinary Physiology, College of Veterinary Science, Punjab Agricultural University)
  • Received : 2002.06.18
  • Accepted : 2002.10.18
  • Published : 2003.02.01


The present investigation was undertaken to study status of erythrocytic antioxidant enzymes in normal cycling and $\alpha$- tocopherol supplemented anestrus buffalo heifers. The pre-supplementation erythrocytic activities of superoxide dismutase (U/mg Hb), glutathione peroxidase (U/mg Hb) and glucose-6-phosphate dehydrogenase (U/g Hb) upregulated significantly (p<0.05) in anestrus heifers ($10.08{\pm}0.09$, $14.09{\pm}0.54$, $9.25{\pm}0.29$) when compared to normal cycling ones ($6.93{\pm}0.04$, $11.61{\pm}0.19$, $5.58{\pm}0.26$). The oral supplementation of $\alpha$-tocopherol (a) 3,000 mg per week per animal in anestrus heifers declined erythrocytic superoxide dismutase and glucose-6-phosphate dehydrogenase activities significantly (p<0.01) but led to non-significant increase in erythrocytic glutathione peroxidase activity. Results indicated that supplementation of $\alpha$-tocopherol to anestrus buffalo heifers mitigated the effects of oxidative stress to improve their antioxidant status.


Antioxidant Enzyme;$\alpha$-Tocopherol;Anestrus;Buffalo Heifers


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