DOI QR코드

DOI QR Code

Effects of Scutellariae Radix-acupuncture solution On Anti-inflammatory in RAW 264.7 macrophages

황금약침액(黃芩藥針液)이 LPS로 유도(誘導)된 RAW 264.7 대식세포(大食細胞)에서의 항염증효과(抗炎症效果)

  • Choi, Young-Kwang (Department of Meridian & Acupoint, College of Oriental Medicine, Semyung University) ;
  • Park, Sung-Ho (Department of Meridian & Acupoint, College of Oriental Medicine, Semyung University) ;
  • Seo, Il-Bok (Department of Anatomy, College of Oriental Medicine, Semyung University) ;
  • Kim, Ho-Hyun (Department of Physiology, College of Oriental Medicine, Semyung University) ;
  • Kim, Jeong-Seon (Department of Oriental Medical Food & Nutrition, Semyung University) ;
  • Kim, Ee-Hwa (Department of Anatomy, College of Oriental Medicine, Semyung University)
  • 최영광 (세명대학교 한의과대학 경혈학교실) ;
  • 박성호 (세명대학교 한의과대학 경혈학교실) ;
  • 서일복 (세명대학교 한의과대학 해부학교실) ;
  • 김호현 (세명대학교 한의과대학 생리학교실) ;
  • 김정선 (세명대학교 한방식품영양학과) ;
  • 김이화 (세명대학교 한의과대학 해부학교실)
  • Published : 2003.12.30

Abstract

Objective : Recently, Herbal-acupuncture therapeutics has been used for the treatment of inflammatory diseases such as rheumatoid arthritis. Especially, we have been interested in chemical mediators concerned with inflammation such as prostaglandin, cytokine, nitric oxide. The purpose of this study is investigated that the effect of Scutellariae Radix-acupuncture solution in lipopolysaccharide-stimulated RAW 264.7 macrophages, performed several expeimental items : those are prostaglandin E. nitric oxide and cyclooxygenase-2. Method : The cytotoxicity of Scutellariae Radix-acupuncture solution in RAW 264.7 macrophages were measured by MTT-based cytotoxicity assay. In order to observe cyclooxygenase-2 mRNA expression in lipopolysaccharide-stimulated RAW 264.7 macrophages, RT-PCR was used. Prostaglandin $E_2$ formation and nitric oxide production was measured by competitive enzyme immunoassay and Griess assay. Results : 1. The MTT assay demonstrated that cytotoxic effect of Scutellariae Radix-acupuncture solution in RAW 264.7 macrophages were not appeared before concentration of 5mg/mL 2. Scutellariae Radix-acupuncture solution inhibited cyclooxygenase-2 mRNA expression in lipopolysaccharide-treated RAW 264.7 macrophages. 3. Scutellariae Radix-acupuncture solution inhibited nitric oxide production in lipopolysaccharide-treated RAW 264.7 macrophages. 4. Scutellariae Radix-acupuncture solution inhibited prostaglandin $E_2$ formation in lipopolysaccharide-treated RAW 264.7 macrophages. Conclusion : On the basis of these results, It was shown that Scutellariae Radix-acupuncture solution is significantly able to inhibit the production of $PGE_2$ and NO, as well as COX-2 mRNA expression. Our results may provide new mechanism by which Scutellariae Radix-acupuncture solution accounts for its beneficial effect on accelerating wound healing and anti-inflammation.

References

  1. 대한병리학회. 병리학. 서울: 고문사. 1995 : 83-89.
  2. Chen YC, Shen SC, Chen LG, Lee TJ, Yang LL. Wogonin, baicalein and baicalin inhibition of inducible nitric oxide synthase and cyclooxygenase-2 gene expressions induced by nitric oxide synthase inhibitors and lipopolysaccharide. Biochem Pharmacol 2001;61:1417–1427.
  3. Martin-Sanz P, Calles NA, Diaz-Guerra MJ, Bosca L. Expression of cyclooxgenase-2 in foetal rat hepatocytes stimulated with lipopolysaccharide and pro-nflammatory cytokines. Br J Pharmacol 1998;125:1313–1319.
  4. Chi YS, Cheon BS, Kim HP. Effect of wogonin, a plant flavon from Scutellariae Radix, on the suppression of cyclooxygenase-2 and the induction of inducible nitric oxide synthase in lipopolysaccharide-treated RAW 264.7 cells. Biochem Pharmacol 2001;61:1195–1203. https://doi.org/10.1016/S0006-2952(01)00597-4
  5. Horton JK, Williams AS, Smith-Phillps Z, Matin RC. O'Beirne G: Intracellular measurement of prosaglandin $E_2$: effect of anti-inflammatory drugs cyclooxygenase activity and prostanoid expression. Anal Biochem 1999;271(1):18–28. https://doi.org/10.1006/abio.1999.4118
  6. Chou TZC, Earl Fu, Shen EC. Chitosan inhibits PGE2 formation and COX-2 induction in lipopolysaccharide-treated RAW 264.7 macrophages. Biochem Biophys Res Com 2003;308:403–407. https://doi.org/10.1016/S0006-291X(03)01407-4
  7. 노태철, 최희철, 김보연, 김영호, 안종석, 김영국, 이현선. 쿠마린에 의한 RAW 264.7 세포주의 Nitric Oxid 생성 저해활성. 생약학회지 1999;30(4):413–416.
  8. Dubois RN, Abramson SB, Crofford L, Gupta RA, Simon LS, Van De Putte LB, Lipsky PE. Cyclooxygenase in biology and disease. FASEB J 1998;12(12):1063–1073.
  9. Kufmann WE, Andresson KI, Isakson PC, Worley PF. Cyclooxygenase and the central nervous system. Prostaglandins 1997;54(3):601–624. https://doi.org/10.1016/S0090-6980(97)00128-7
  10. Moncada S, Higgs A. The L-arginine nitric oxide pathway. N ENGL J MED 1993;329:2002–2012.
  11. Knowles RG, Moncada S. Nitric oxide as a signal in blood vessels. TIBS 1992;17:399–402. https://doi.org/10.1016/0968-0004(92)90008-W
  12. 전국한의과 대학 본초학교실. 본초학. 서울. 영림사. 1994 : 178-179.
  13. 구민숙, 윤종화, 김경호, 장준혁, 이승덕, 김갑성. 호도약침이 생쥐의 Adjuvant 관절염에 미치는 영향. 대한침구학회지 2002;19(3):88–94.
  14. 장통영. 牛膝藥鍼이 Rat의 Adjuvant 관절염에 미치는 영향. 대한한의학회지 2000;21(3):77–87.
  15. 李鐘國. 防風藥鍼이 진통, 소염, 해열 및 진경에 미치는 영향. 대한침구학회지 1986;3:11–23.
  16. 박순영. 의학통계학. 서울. 경희대학교출판국. 1998. 162-183.
  17. Insel PA, Analgesic-antipyretic and drugs employed in the treatment of gout, In : Hardman JG, et al., The pharmacological basis of therapeutics. 9th ed.. USA : McGraw-Hill. 1996 ; 617-624.
  18. 염정호. 니켈 및 코발트의 세포독성 기전에서 NO의 역할. 대한 산업의학회지 2001 ; 13(3) : 274–277.
  19. Stuehr D, Cho HJ, Kwon NS, Weise M. Nathan CF. Purification and characterization of the cytokine induced macrophage NO synthase: an FAD- and FMN-containing flavoprotein. Proc Natl Sci USA 1991 ; 88 : 7773–7777.
  20. 김영덕, 전창덕, 이병순, 이복수, 박석돈, 백상기, 정헌택. 사이토카인과 LPS 자극에 의한 RAW 264.7 세포주의 NO 생성. 대한면역학회지 2002 ; 10 : 345–350.
  21. Kimuya Y, Kubo M, Tani T, Arichi S, Okuda H. Studies on Scutellariae Radix. IV. Effect on lipid peroxidation in rat liver. Chem Pharm Bull (Tokyo) 1981;29(9):2610–2617.
  22. 문관성. 약초의 성분과 이용. 평양. 과학백과사전출판사. 1999:618-620.
  23. 신민교. 원색임상본초학. 서울. 영림사. 1992:308-10.
  24. 대한통증학회. 통증의학. 서울. 군자출판사. 2000:8-26.
  25. 류은경, 최도영, 이재동. 세포증식과 COX-2 발현에 미치는 蜂毒의 효과. 대한침구학회지 2003;20(2):112–122.
  26. 황대연, 김호현, 김창주, 김이화. 蜂毒이 골육종 세포주에서 세포사멸 및 COX-2 억제에 미치는 영향. 대한침구학회지 2003;20(3):63–74.
  27. Giatgen A. The Dual Role of nitric oxide in Islet $\beta$-cell. New Physiol Sc 1999 ; 14 : 49–53.
  28. Feldman PL, Griffith OW, Sheuhr DJ. The surprising life of nitric oxide. Chem Eng News 1993 : 26–38.
  29. 김동현, 서정철, 임성철, 정태영, 한상원. 白何首烏藥鍼液의 NO, DPPH 소거 및 IL-4 억제효과. 대한침구학회지 2003 ; 20(4) : 45–52.
  30. 이경민, 서정철, 한상원. 황기약침액의 NO, DPPH 소거 및 IL-4 억제효과. 대한침구학회지 2003 ; 20(2) : 184–194.
  31. 조현석, 김갑성. 전갈약침액의 인체연골세포에서 nitric oxide synthase의 IL-1 유도 유전형질 발현과 nitric oxide의 생산의 억제에 관한 연구. 대한침구학회지 2003 ; 20(1) : 104–119.