Synergistic action of pesticide mixtures using glutathione-s-transferase- and esterase-inhibiting properties in diamondback moth (Plutella xylostella L.)

Glutathione-S-transferase와 esterase 효소 저해특성을 이용한 농약의 혼합 상승효과

  • Yu, Yong-Man (Central Institute, Kyungnong Co.) ;
  • Hong, S.S. (Division of Biological Environment, Kangwon National University) ;
  • Kim, S. (Division of Biological Environment, Kangwon National University) ;
  • Hur, J.H. (Division of Biological Environment, Kangwon National University)
  • 유용만 ((주) 경농 중앙연구소) ;
  • 홍순성 (강원대학교 농업생명과학대학 생물환경학부) ;
  • 김성문 (강원대학교 농업생명과학대학 생물환경학부) ;
  • 허장현 (강원대학교 농업생명과학대학 생물환경학부)
  • Published : 2003.03.27

Abstract

In vitro inhibitory activity of 34 insecticides and 31 fungicides to glutathione-S-transferase and esterases extracted from rats was determined. Of tested pesticides, the pesticides with high activity on both detoxifying enzymes were mixed with pesticides that are known to be detoxified by detoxifying enzymes. Glutathione-S-transferase was inhibited by thiodicarb $(I_{50}:1.87\times10^{-4}M)$, thiocyclam $(7.40\times10^{-4}M)$, dithianon $(7.55\times10^{-5}M)$, and tolylfluanide $(8.66\times10^{-5}M)$, while esterases by dichlorvos $(8.95\times10^{-8}M)$, pirimicarb $(2.74\times10^{-6}M)$, pyrazophos $(3.31\times10^{-5}M)$, and benomyl $(4.96\times10^{-5}M)$. After acephate known to be detoxified by glutathione-S-transferase was mixed with glutathione-S-transferase-inhibiting pesticides and phenthoate known to be detoxified by esterases was mixed with esterases-inhibiting pesticides, insecticidal activities of such mixtures were determined against diamondback moth (PlutelLa xylostella L.). Synergistic action was observed in all pesticide combinations. The highest synergistic action was obtained when phenthoate was combined with dichlorvos, showing that co-toxicity coefficients were 1512 and 1877 after 24 and 48 hours of treatment, respectively. Several other combinations of pesticides, such as phenthoate with benomyl, and acephate with dithianon, also showed synergism, showing that their co-toxicity coefficients were about 1,000 and 500, after 24 hours of treatment, respectively. Our results showed that combinations of pesticides inhibited by detoxifying enzymes and ones detoxified by detoxifying enzymes resulted in increased toxicities of pesticides, suggesting that such combinations could be used to develop pesticide mixtures with more broad spectrum and high effectiveness.

References

  1. Dautennan, W. C. (1994) Introduction to biochemical toxicity. pp.542-577, Detoxication metabolisms in insect
  2. Gopallan, N., B. K. Bhattacharya, S. Prakash, and K. M. Rao (1997) Characterization of carboxylesterases from malathion-resistant Culex quinquefasciatus Say (Diptera: Culicidae) mosquitoes. Pestic. Biochem. Physiol. 57:99-108
  3. Habig, W. H., M. J. Pabst, and W. B. Jakoby (1974) GST : The first step in mercapturic acid formation, J. BioI. Chem. 249:7130-7146
  4. Hodson, E. and P. E. Levi (1994) Introduction to biochemical toxicology (2nd). pp.75-132. Metabolism of toxicants
  5. Hughes, P. B. and D. A. Raftos (1985) Genetics of an esterase associated with resistance to organophosphorus insecticides in the sheep blow fly, Lucilia cuprina (WiedemAnn) (Diptera:Calliphoridae). Bull. Ent. Res. 75:535
  6. Johnson, E. R. and Y. P. Sun (1960) Analysis of joint action of insecticides against house flies. J. Econ, Entomol. 53(5):887-889
  7. Lamoureux, G. L. and D. G. Rusness (1987) Synergism of diazinon toxicity and inhibition of diazinon metabolism in the house fly by tridiphane: inhibition of glutathion-S-transferase activity. Pestic. Biochem. Physiol, 27:318- 329
  8. Lowry, O. H., N. J. Rosebrough, and R. J. Randell (1951) Protein measurement with the folin phenol reagent. J. BioI. Chem. 193:265-275
  9. Payne, G. T. and T. M. Brown (1984) EPN and S,S,S-tributyl phosphorotrithioate as synergist of methyl paration in resistance tabacco budwonn larvae (Lepidoptera:Noctuidae). J. Econ, Entomol. 77:294-287
  10. Van Asperin (1962) A study of housefly esterases by means of a sensitive colorimetric method. J. Insect Physiol. 8:401-416
  11. Yasuhiko, K. and T. Shishido (1996) Glutathiondependent O-alkyl and O-aryl conjugations for dicapton and fenitrothion in several insect. J. Pesticide Sci. 21:430-433
  12. Yu, S. J., F. A. Robinson, and J. L. Nation (1984) Detoxication capacity in the honybee (Apis mellifera L.), Pestic. Biochem. Physiol., 22:360-368
  13. 김용균, 장동걸 (1996) 배추좀나방의 deltamethrin 저항성 기작에 관한 esterase의 역할. 한국응용곤충학회지 35(1):74-79
  14. 박종열, 이형래, 검정화 (1993) 바퀴(Blattela germanicaL)의 살충제 저항성에 관한 연구-1. 생물검정 방법에 따른 살충력 비교. 한국응용곤충학회지 32(1):24-29
  15. 송승석, 오홍규, Naoki Motoyama (1993) C따boxylesterase의 활성측정 에의한 복숭아혹 진딧물, Myzus perrsicae S.의 살충제 포장 저항성도의 계절적 변동. 한국응용곤충학회지 32(3):348-353