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브로콜리 용매추출물의 Bioactive Organosulfur 화합물의 분리 및 동정

Isolation and Identification of Bioactive Organosulfur Phytochemicals from Solvent Extract of Broccoli

  • 발행 : 2003.04.01

초록

브로콜리 중의 bioactive organosulfur phytochemicals를 분석하기 위해 브로콜리를 용매추출하여 GC/MSD에 의해 분리동정하였다. 브로콜리에 함유된 phytochemicals 중에서 isothiocyanate류가 40.42%로 대부분을 차지하였으며, glucosinolate분해산물인 nitrile류는 5.12%로 isothiocyanate류에 비해 적었고, sulfide류는 0.84%로 매우 적었다. 분리 동정된 isothiocyanate류의 종류로는 3-butenyl 4-methtlthiobutyl, 4-methrlthio -3- butenyl, 5- methylthiopentyl, 2- phenylethyl, 3-methylsulfinyl propyl 및 4-methylsulfinylbutyl isothiocyanate의 7종이었다. Isothiocyanate류 중에서 3-butenyl isothiocynate는 22.05%로 가장 많았으며, 그 다음으로 sulforaphane 16.50%로 2종의 isothiocyanate가 대부분을 차지하였다. Nitrile의 종류는 4-methylthiobutyl, 5-methylthiopentyl, 2-phenylethyl 및 4-methylsulfinylbutyl nitrile의 6종류가 확인되었다. 한편, 동정된 sulfide종류로는 dimethyl disulfide, dimethyl trisulfide 및 dimethyl tetrasulfide이었다.

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  2. Cosmeceutical Activity of Broccoli (Brassica oleracea var. italica Plenck) with Different Light Sources vol.22, pp.3, 2012, https://doi.org/10.5352/JLS.2012.22.3.347
  3. Antioxidant Activity of Solvent Fraction from Broccoli Sprouts Cultivated at the Plant Factory System vol.28, pp.1, 2015, https://doi.org/10.9799/ksfan.2015.28.1.001
  4. Fermentation Characteristics and anti-Helicobacter pylori Activity of Aqueous Broccoli Fermented by Lactobacillus plantarum MG208 vol.58, pp.1, 2015, https://doi.org/10.3839/jabc.2015.015
  5. Quality and Antioxidant Properties of White Breads Enhanced with Broccoli (Brassica oleracea L.) Powder vol.31, pp.5, 2015, https://doi.org/10.9724/kfcs.2015.31.5.614
  6. Antimicrobial and Antioxidant Activities in Different Parts and Cultivars of Broccoli vol.32, pp.3, 2014, https://doi.org/10.7235/hort.2014.13183
  7. Physicochemical and Antioxidant Properties of Broccoli Sprouts Cultivated in the Plant Factory System vol.28, pp.1, 2013, https://doi.org/10.7318/KJFC/2013.28.1.057
  8. Effect of Cake Improver on Antioxidant Activity and Properties Characteristics of Pound Cakes Prepare using Broccoli Stem Powder vol.24, pp.4, 2011, https://doi.org/10.9799/ksfan.2011.24.4.567