DOI QR코드

DOI QR Code

고아밀로오스전분의 섭취가 흰쥐의 지방대사 및 면역능력에 미치는 영향

Effects of High Amylose Starch on Lipid Metabolism and Immune Response in Rats

  • 설소미 (단국대학교 식품영양학과) ;
  • 방명희 (단국대학교 식품영양학과) ;
  • 최옥숙 (단국대학교 식품영양학과) ;
  • 김우경 (단국대학교 식품영양학과)
  • 발행 : 2003.04.01

초록

본 연구는 SD계 흰쥐를대상으로 전분을 식이 무게의 50%로 주면서 일반 옥수수전분을 고아밀로오스 옥수수전분으로 대치시 켜 RS양을 증가시켰을 때, 지방대사와 면역능력에 미치는 영향을 살펴보는 것을 목적으로 하였다. 식이섭취량이나 식이 효율에는 유의적인 차이가 없었으나 최종 체중은 대조군에 비해 HA100군에서 감소하는 경향이었다. 맹장무게와 하루 변배설량은 식이내 HAS량이 증가할수록 유의적으로 증가하였다. 맹장내용물의 pH는 식이 내 HAS량이 증가할수록 유의적으로 감소하였다. 혈장 내 총 지방, 중성지방, 총 콜레스테롤량은 HAS섭취가 증가하면 감소하였으나 HDL-콜레스테롤량과 간내 총 지방, 중성 지방, 콜레스테롤량은 HAS섭취에 의한 영향을 받지 않았다. 변을 통한 총 콜레스테롤의 배설량은 HA50군에서 대조군에 비해 유의적으로 증가하였다. 면역 기관인 흉선과 비장의 무게와 혈장의 Ig G와 $C_3$농도는 식이에 의해 영향을 받지 않았다. 비장세포의 증식 능력을 보았을 때 PHA에 의한 자극에는 식이의 영향이 없었으나 저용량의 Con A로 자극하였을 때 HA25, HA50에서 증식이 유의적으로 낮았고, 배양액 내 PGE$_2$의 생산량이 증가하는 경향을 보였다. 이를 종합해보면, HAS의 섭취는 체중감소의 효과가 있는 것으로 보이며, 맹장의 무게를 증가시키고, 맹장 내 pH를 낮추고, 혈액내 지방성분을 감소시키는 것으로 나타났다.

참고문헌

  1. Asp NG. 1997. Resistant starch--an update on its physiological effects. Adv Exp Med Biol 427: 201-210. https://doi.org/10.1007/978-1-4615-5967-2_21
  2. Marteau P, Flourie B. 2001. Tolerance to low-digestible carbohydrates: symptomatology and methods. Brit J Nutr 85 (suppl 1): S17-S21. https://doi.org/10.1079/BJN2000258
  3. Murphy O. 2001. Non-polyol low-digestable carbohydrates: food applications and functional benefits. Brit J Nutr 85 (suppl 1): S47-S53. https://doi.org/10.1079/BJN2000261
  4. Nuir JG, Birkett A, Brown I, Jones G, O'Dea K. 1995. Food processing and maize variety affects of starch escaping digestion in the small intestine. Am J Clin Nutr 61: 82-89. https://doi.org/10.1093/ajcn/61.1.82
  5. Behall KM, Howe JC. 1996. Resistant starch as energy. J Am Coll Nutr 15: 248-254. https://doi.org/10.1080/07315724.1996.10718595
  6. Englyst HN, Trowell H, Southgate DAT, Cummings JH. 1987. Dietary fiber and resistant starch. Am J Clin Nutr 46: 873-874. https://doi.org/10.1093/ajcn/46.6.873
  7. Haralampu SG. 2000. Resistant starch-a review of physical properties and biological impact of RS3. Carbohydrate polymers 41: 285-292. https://doi.org/10.1016/S0144-8617(99)00147-2
  8. Tovar J, Björck I, Asp NG. 1992. Starch content and alphaamylolysis rate in precooked legume flours. J Agric Chem 38: 1818-1823. https://doi.org/10.1021/jf00099a007
  9. Sievert D, Pomeranz Y. 1989. Enzyme-resistant starch I. Characterization and evaluation by enzymatic, thermoanalytical and microscopic methods. Cereal Chem 66: 342-347.
  10. Englyst HN, Kingman SM, Cummings JH. 1992. Classification and measurement of nutritionally important starch fractions. Eur J Clin Nutr 46 (suppl 2): S30-S50.
  11. Behall KM, Howe JC, Anderson RA. 2002. Apparent mineral retention is similar in control and hyperinsulinemic men after consumption of high amylose cornstarch. J Nutr 132: 1886-891. https://doi.org/10.1093/jn/132.7.1886
  12. Tatsuya M, Seiichi K, Koji H, Shuhachi K. 1999. Psyllium shifts the fermentation site of high amylose cornstarch toward the distal colon and increase fecal butyrate concentration in rats. J Nutr 129: 2081-2087. https://doi.org/10.1093/jn/129.11.2081
  13. Kabir M, Rizkalla SW, Champ M, Luo J, Boilloy J, Bruzzo F, Slama G. 1998. Dietary amylose-amylopectin starch content affects glucose and lipid metabolism in adipocytes of normal and diabetic rats. J Nutr 128: 35-43. https://doi.org/10.1093/jn/128.1.35
  14. Reeves PG, Nielsen FH, Fahey GC Jr. 1993. AIN-93 Purified Diets for Laboratory Rodents: Final Report of the American Institute of Nutrition Ad Hoc Writing Committee on the Reformulation of the AIN-76A Rodent Diet. J Nutr 123: 1939-1951. https://doi.org/10.1093/jn/123.11.1939
  15. AOAC. 1990. Official methods of analysis. 15th ed. Total dietary fiber in food, enzymatic-gravimetric method. The association of official analytical chemists, Washington DC, USA. p 1105-1106.
  16. Chen HL, Lu YH, Lin J, Ko LY. 2000. Effects of fructooligosaccharide on bowel function and indicators of nutritional status in constipated elderly men. Nutr Res 12: 1725-1733.
  17. Folch JM, Lees G, Stanley HS. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J Bio Chem 223: 497-509.
  18. Zak B. 1968. Total and free cholesterol. In Standard method chemistry. Acad Press, NY. p 79-89.
  19. Sternberg JC. 1977. A rate nephelometer for measuring specific proteins by immunoprecipitate reaction. Clin Chem 23: 1456-1464.
  20. Topping DL, Gooden JM, Brown IL, Biebrick DA, McGrath L, Trimble RP, Choct M, Illman RJ. 1997. A high amylose (Amylomaize) starch raises proximal large bowel starch and increases colon length in pig. J Nutr 127: 615-622 https://doi.org/10.1093/jn/127.4.615
  21. Schrijver R, Vanhoof KM, Vande GJ. 1999. Nutrient utilization in rats and pigs fed enzyme resistant starch. Nutr Res 19: 1349-61. https://doi.org/10.1016/S0271-5317(99)00092-5
  22. Ranhotra GS, Gelroth JA, Glaser BK. 1996. Energy value of resistant starch. J Food Sci 61: 453-455. https://doi.org/10.1111/j.1365-2621.1996.tb14215.x
  23. Morita T, Kasaoka S, Hase K, Kiriyama S. 1999. Psyllium shifts the fermentation site of high amylose cornstarch toward the distal colon and increases fecal butyrate concentration in rats. J Nutr 129: 2081-2087. https://doi.org/10.1093/jn/129.11.2081
  24. Ferguson LR, Jones CT, Englyst H, Harris PJ. 2000. Comparative effects of three resistant starch preparations on transit time and short chain fatty acid production in rats. Nutr Cancer 36: 230-237. https://doi.org/10.1207/S15327914NC3602_13
  25. Phillips J, Muir J, Birkett A, Lu ZX, Jones GP, O'Dea K. Young GP. 1995. Effect of resistant starch on fecal bulk and fermentation dependent events in humans. Am J Clin Nutr 62: 121-130. https://doi.org/10.1093/ajcn/62.1.121
  26. Wang X, Brown IL, Khaled D, Mahony MC, Evans AJ, Conway PL. 2002. Manipulation of colonic bacteria and volatile fatty acid production by dietary high amylose maize (amylomaze) starch granules. J Appl Microbiol 93: 390-397. https://doi.org/10.1046/j.1365-2672.2002.01704.x
  27. Noakes M, Clifton PM, Nestel PJ, Le Leu R, Mclintosh G. 1996. Effect of high amylose starch and oat bran on metabolic variables and bowel function in subjects with hyperglyceridemia. Am J Clin Nutr 64: 944-951. https://doi.org/10.1093/ajcn/64.6.944
  28. Heijen MLA, van Amelsvoort JMM, Deurenberg P, Beynen AC. 1998. Limited effect of consumption of uncooked $(RS_2)$ or retrograded $(RS_3)$ resistant starch on putative risk factors for colon cancer in healthy men. Am J Clin Nutr 67: 322-331. https://doi.org/10.1093/ajcn/67.2.322
  29. Ranhotra GS, Gelroth JA, Leinen SD. 1997. Hypolipidemic effects of resistant starch in hamsters is not dose dependent. Nutr Res 17: 317-323. https://doi.org/10.1016/S0271-5317(96)00261-8
  30. Younes H, Levrat M, Demigne C, Remest C. 1995. Resistant starch is more effective than cholestyramine as a lipid-lowering agent in the rats. Lipids 30: 847-853. https://doi.org/10.1007/BF02533961
  31. Jeong MK, Kim MH, Kang NE, Kim WK. 2002. Effects of resistant starch on gut functions and plasma lipid profiles in rats fed high fat diet. J Korean Soc Food Sci Nutr 31: 271-276. https://doi.org/10.3746/jkfn.2002.31.2.271
  32. Lopez HW, Levrat-verny MA, Coudray C, Besson C, Krespine V, Messager A, Demigne C. 2001. Class 2 resistant starches lower plasma and liver lipids and improve mineral retention in rats. J Nutr 131: 1283-1289. https://doi.org/10.1093/jn/131.4.1283
  33. Vanhoof K, Schrijver R. 1997. Consumption of enzyme resistant starch and cholesterol metabolism in normo-and hypercholesterolemic rats. Nutr Res 17: 1331-1340. https://doi.org/10.1016/S0271-5317(97)00117-6
  34. Reiser S, Powell AS, Scholfield DJ, Panda P, Ellwood KC, Canary JJ. 1989. Blood lipids, lipoproteins, apoproteins and uric aicd in men fed diets containing high fructose or high amylose cornstarch. Am J Clin Nutr 49: 832-839. https://doi.org/10.1093/ajcn/49.5.832
  35. Marchini SJ, Faisant N, Champ M, Ranganathan S, Azoulay C, Kergueris MF, Piloquet H, Michel K. 1998. Effects of an acute raw resistant potato starch supplement on postprandial glycemia, insulinemia, lipemia in healthy adults. Nutr Res 18: 1135-1145. https://doi.org/10.1016/S0271-5317(98)00095-5
  36. Morand C, Levart MA, Besson C, Demigne C, Remesy C. 1994. Effects of diet rich in resistant starch on hepatic lipid metabolism in rats. J Nutr Biochem 5: 138-144. https://doi.org/10.1016/0955-2863(94)90085-X
  37. Chezem J, Furumoto E, Story J. 1997. Effects of resistant potato starch on cholesterol and bile acid metabolism in the rat. Nutr Res 17: 1671-1682. https://doi.org/10.1016/S0271-5317(97)00174-7
  38. Abadie C, Hug M, Kubi C, Gains N. 1994. Effect of cyclodextrins and undigested starch on the loss of chenodeoxycholate in the faces. Biochem J 299: 725-730. https://doi.org/10.1042/bj2990725
  39. Chen WJL, Anderson JW, Jennings D. 1984. Propionate may mediate the hypocholesterolemic effects of certain soluble plant fibers in cholesterol fed rats. Proc Soc Exp Biol Med 175: 215-218. https://doi.org/10.3181/00379727-175-41791
  40. Hylla S, Gostner A, Dusel C, Anger H, Bartran HP, Christl SU, Kasper H, Scheppach W. 1998. Effects of resistant starch on the colon in healthy volunteers; possible implications for cancer prevention. Am J Clin Nutr 67: 137-142.
  41. Vanhoof K, De Schrijver R. 1998. The influence of enzymeresistant starch on cholesterol metabolism in rats fed on conventional diet. Br J Nutr 80: 193-198.
  42. Fernandez ML, Roy S, Vergara-Jimenez M. 2000. Resistant starch and cholestyramine have distinct effects on hepatic cholesterol metabolism in guinea pigs fed a hypercholesterolemic diet. Nutr Res 20: 837-849. https://doi.org/10.1016/S0271-5317(00)00170-6
  43. Kurita-Ochiai T, Fukushima K, Ochiai K. 1995. Volatile fatty acids, metabolic by-products of periodontopathic bacteria, inhibit lymphocyte proliferation and cytokine production. J Dent Res 74: 1367-1373. https://doi.org/10.1177/00220345950740070801
  44. Eftimiadi C, Stashenko P, Tonetti M, Mangiante PE, Massara R, Zupo S, Ferrarini M. 1991. Divergent effect of the anaerobic bacteria by-product butyric acid on the immune response: suppression of T-lymphocyte proliferation and stimulation of interleukin-1 beta production. Oral Microbiol Immunol 6: 17-23. https://doi.org/10.1111/j.1399-302X.1991.tb00446.x
  45. Kurita-Ochiai T, Ochiai K, Fukushima K. 1998. Volatile fatty acid, metabolic by-product of periodontopathic bacteria, induces apoptosis in WEHI 231 and RAJI B lymphoma cells and splenic B cells. Infect Immun 66: 2587-2594.
  46. Kudoh K, Shimizu J, Wada M, Takita T, Kanke Y, Innami S. 1998. Effect of indigestible saccharides on B lymphocyte response of intestinal mucosa and cecal fermentation in rats. J Nutr Sci Vitaminol (Tokyo) 44: 103-112. https://doi.org/10.3177/jnsv.44.103
  47. Lom BO, Yamada K, Nonaka M, Kuramato Y, Hung P, Sugano M. 1997. Dietary fibers modulate indices of intestinal immune function in rats. J Nutr 127: 663-667. https://doi.org/10.1093/jn/127.5.663

피인용 문헌

  1. Physicochemical Properties of Rice-based Expanded Snacks according to Extrusion Conditions vol.43, pp.9, 2014, https://doi.org/10.3746/jkfn.2014.43.9.1407
  2. The effect of chemically-modified resistant starch, RS type-4, on body weight and blood lipid profiles of high fat diet-induced obese mice vol.64, pp.1, 2012, https://doi.org/10.1002/star.201100057
  3. Effects of Agarum cribrosum on the Improvements in Treating Constipation and Plasma lipid Profiles vol.29, pp.2, 2013, https://doi.org/10.9724/kfcs.2013.29.2.185
  4. Effect of Resistant Starch on the Large Bowel Environment and Plasma Lipid in Rats with Loperamide-Induced Constipation vol.39, pp.5, 2010, https://doi.org/10.3746/jkfn.2010.39.5.684