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Micromachined DNA Manipulation Device Using Circular Multi-Electrodes

원형 다중전극을 이용한 DNA 조작소자

  • 문상준 (포항공과대학교 대학원 기계산업공학부) ;
  • 윤재영 (포항공과대학교 대학원 생명과학부) ;
  • 남홍길 (포항공과대학교 대학원 생명과학부) ;
  • 지연태 (전남대학교 대학원 유전공학과) ;
  • 이승섭 (포항공과대학교 기계공학과)
  • Published : 2003.07.01

Abstract

In this paper, we present a DNA manipulation device in the reaction chamber, which consists of a center electrode and circular outer electrodes of a reaction unit. The charged bio-molecules, DNA, are manipulated by the charge of the electrode in reaction unit. Controlling the induced dynamic electric field between the center electrode and the outer electrodes, concentration / repulsion / manipulation of bio-molecules are enabled at a periphery of electrode. Concentration of the fluorescent DNA at the center electrode is observed by applying +2V. Subsequently, applying -2V, the concentrated DNA is repelled rapidly from the center electrode, which makes dispersion completely in 0.5second. Furthermore, repeated applying +1V/-1V every 5 seconds at each outer electrode, we can circulate the DNA. We also investigate a micro-heater and sensor for DNA manipulation and reaction temperature. The coefficient of heat-resistance and heater temperature characteristic is 0.0043 and 100$^{\circ}C$/sec, respectively.

Keywords

Circular Multi-Electrodes;DNA Manipulation;Fluorescence;Micro-Heater

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