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Production of L-α-Glycerophosphate Oxidase by streptococcus faecium M74 · LC

Streptococcus faecium M74 · LC에 의한 L-α-Glycerophosphate Oxidase의 생산

  • Lee, In-Ae (Institute of Agricultural Science and Technology, Kyungpook National University) ;
  • Lee, Eun-Sook (Dept. of Preparatory Oriental Medicine, Kyungsan University) ;
  • Lee, June-Woo (Dept. of Food Processing and Cooking, Kyungbuk College)
  • 이인애 (경북대학교 농업과학기술연구소) ;
  • 이은숙 (경산대학교 한의예과) ;
  • 이준우 (경북전문대학 식품가공조리과)
  • Published : 2003.07.01

Abstract

The objective of this study was to search for the best strain as a source of L- $\alpha$-glycerophosphate oxidase (GPO) production and to establish the process technology for the purification of GPO on an industrial scale. The GPO was produced by culturing Streptococcus faecium, and purified by ammonium sulfate, DEAE-cellulose and hydroxyapatite chromatography. The relative activity was 60 units/L for 5. faecim ATCC 12755, 65 units/L for 5. faecium ATCC 19634, and 67 units/L for 5. faecium $M_{74}$.LC, respectively. The optimum condition for fermentation was $37^{\circ}C$ for temperature, 300 rpm for stir rate, 0.5 L/min for aeration rate and 17 hours. The main culture medium prepared by the modified AC medium. AC medium consists of 0.1% glucose, 0.2% glycerol, 1.0% tryptone and 1.0% yeast extract, 0.5% $K_2HP0_4$, pH 7.0. The GPO was purified by ammonium sulfate fractionation and ion exchange column chromatography, The yield and purity were 17.2% and 5.3 fold, respectively.

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