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Inhibitory Effect of Hot-Water Extract of Paeonia japonica on Oxidative Stress and Identification of Its Active Components

백작약 열수추출물의 산화적 스트레스 억제효과 및 유효성분 동정

  • Jeong, Ill-Yun (Radiation Food Technology and Bioscience Team, Korea Atomic Energy Research Institute) ;
  • Lee, Joo-Sang (Radiation Food Technology and Bioscience Team, Korea Atomic Energy Research Institute) ;
  • Oh, Heon (Radiation Food Technology and Bioscience Team, Korea Atomic Energy Research Institute) ;
  • Jung, U-Hee (Radiation Food Technology and Bioscience Team, Korea Atomic Energy Research Institute) ;
  • Park, Hae-Ran (Radiation Food Technology and Bioscience Team, Korea Atomic Energy Research Institute) ;
  • Jo, Sung-Kee (Radiation Food Technology and Bioscience Team, Korea Atomic Energy Research Institute)
  • 정일윤 (한국원자력연구소 방사선식품.생명공학기술 개발팀) ;
  • 이주상 (한국원자력연구소 방사선식품.생명공학기술 개발팀) ;
  • 오헌 (한국원자력연구소 방사선식품.생명공학기술 개발팀) ;
  • 정우희 (한국원자력연구소 방사선식품.생명공학기술 개발팀) ;
  • 박혜란 (한국원자력연구소 방사선식품.생명공학기술 개발팀) ;
  • 조성기 (한국원자력연구소 방사선식품.생명공학기술 개발팀)
  • Published : 2003.07.01

Abstract

This study was carried out to investigate the antioxidative activity and to identify the active components of hot-water extract of Paeoniajaponica (PJ), which was a main ingredient of a herb mixture preparation recently established as a potent candidate of radioprotector in our laboratory. The water extract was fractionated with CHCl$_3$, EtOAc and n-BuOH. The extract and its fractions showed very low activity in hydroxyl radical scavenging test. In lipid peroxidation test, the extract, EtOAc and water fractions showed moderate inhibition with the ratio above 50%. In DPPH radical scavenging test, the extract, EtOAc and water fraction showed high activity with the ratio above 80%, especially. EtOAc fraction scavenged the radicals as much as synthetic antioxidant (BHA), even at low concentration. It is suggested that mai or partition for antioxidative activity of Paeonia japonica was EtOAc fraction. Subsequently, two active compounds (PJE021-1 and JE024-1) from EtOAc fraction were isolated by using MCI gel and silica gel column chromatography The two compounds inhibited remarkedly the $H_2O$$_2$-induced DNA damage in human peripheral blood lymphocytes, measured by single-cell gel electrophoresis (SCGE). PJE021-1 protected the cells to almost negative control level, dose-dependently. PJE024-1 exhibited a potent inhibition with the ratio of 71% at even low concentration (0.5 $\mu\textrm{g}$/$m\ell$). Finally, their chemical structures were identified as gallic acid (PJE021-1) and (+)-catechin (PJE024-1), respectively, on the basis of the speculation of spectral and physical data.

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