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백작약 열수추출물의 산화적 스트레스 억제효과 및 유효성분 동정

Inhibitory Effect of Hot-Water Extract of Paeonia japonica on Oxidative Stress and Identification of Its Active Components

  • 정일윤 (한국원자력연구소 방사선식품.생명공학기술 개발팀) ;
  • 이주상 (한국원자력연구소 방사선식품.생명공학기술 개발팀) ;
  • 오헌 (한국원자력연구소 방사선식품.생명공학기술 개발팀) ;
  • 정우희 (한국원자력연구소 방사선식품.생명공학기술 개발팀) ;
  • 박혜란 (한국원자력연구소 방사선식품.생명공학기술 개발팀) ;
  • 조성기 (한국원자력연구소 방사선식품.생명공학기술 개발팀)
  • Jeong, Ill-Yun (Radiation Food Technology and Bioscience Team, Korea Atomic Energy Research Institute) ;
  • Lee, Joo-Sang (Radiation Food Technology and Bioscience Team, Korea Atomic Energy Research Institute) ;
  • Oh, Heon (Radiation Food Technology and Bioscience Team, Korea Atomic Energy Research Institute) ;
  • Jung, U-Hee (Radiation Food Technology and Bioscience Team, Korea Atomic Energy Research Institute) ;
  • Park, Hae-Ran (Radiation Food Technology and Bioscience Team, Korea Atomic Energy Research Institute) ;
  • Jo, Sung-Kee (Radiation Food Technology and Bioscience Team, Korea Atomic Energy Research Institute)
  • 발행 : 2003.07.01

초록

백작약(Paeonia japonim)의 열수추출물로부터 산화적 스트레스에 대한 억제 효과를 나타내는 유효 성분을 규명하고자 하였다. 열수추출물은 총매의 극성도에 따라 4종의 분획물(CHCl$_3$, EtOAc, n-BuOH, water)로 구분하였다. 항산화활성 시험 중 hydroxyl 라디칼 소거 시험에서는 열수추출물 및 각 용매 분획물은 모두 아주 낮은 활성을 보였다. 지질 과산화 시험에서는 열수추출물과 EtOAc 및 물 층 분획물이 50%(50 $\mu\textrm{g}$/$m\ell$ 농도에서 ) 이상의 억제활성을 보였다. DPPH 라디칼 소거 시험에서는 열수추출물과 EtOAc 및 n-BuOH 분획물이 80% 이상의 높은 활성을 보였으며, 특히 EtOAc 분획물은 50과 100 $\mu\textrm{g}$/$m\ell$의 농도에서 BHA와 거의 동등한 전자 공여능(EDA값)을 나타내었다. 따라서, EtOAc 분획물에서 2종의 단일 물질을 분리하였다. 분리된 단일 물질인 PJE021-1과 PJE024-l은 10 $\mu\textrm{g}$/$m\ell$의 농도에서 BHA보다 높은 DPPH 라디칼 소거 활성을 나타냈다. PJE021-1은 $H_2O$$_2$에 의한 DNA의 산화적 손상에 대해 최고농도50 $\mu\textrm{g}$/$m\ell$에서 $H_2O$$_2$처리구에 비해 93%의 경감 효과를 나타냈고, PJE024-1은 0.5 $\mu\textrm{g}$/$m\ell$의 낮은 농도에서 71%의 경감 효과를 나타냈다. 이상의 결과로부터 이 2종의 단일 물질이 산화적 스트레스 억제 유효 성분임을 확인하였다. 이 단일 물질은 분광학적기기 분석과 물리적 특성을 분석한 결과 gallic acid(PJE021-1)와 (+)-catechin(PJE024-1)으로 동정되었다. 상기의 결과로부터 백작약은 산화적 스트레스에 대한 경감제로서의 가능성이 시사되었다.

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