Insecticide Susceptibility in the Different Larva of Tobacco Cutworm, Spodoptera litural Fabricius (Lepidoptera: Noctuidae) Collected in the Soybean Fields of Milyang, Korea

밀양산 콩포장 담배거세미나방 유충의 약제에 대한 감수성

  • 배순도 (영남농업시험장 식물환경과) ;
  • 최병렬 (농업과학기술원 작물보호부 농업해충과) ;
  • 송유한 (경상대학교 농업생명과학연구원) ;
  • 김현주 (영남농업시험장 식물환경과)
  • Published : 2003.09.01


The susceptibility of the different larval stages of Spodoptera litura to nine insecticides was evaluated using the perilla leaf-dipping method. Median lethal concentration ($LC_{50}$) was increased with larval development in the range of 0.5 ppm to 5.6 ppm, 9.9 ppm to 27.9 ppm, 9.6 ppm to 125.1 ppm and 24.3 ppm to 546.6 ppm in the 1st, 2nd, 3rd and 4th instar, respectively. The tolerance ratio (TR), which is the TR of 90 percent lethal concentration (LC/ sub 90/) to the recommended concentration, was 0.04 to 0.8 in the 1 st, 0.2 to 7.5 in the 2nd, 0.7 to 115.3 in the 3rd and 1.2 to 485.4 in the 4th instars. Lower D$LC_{50}$ and DTR, which is the difference between the $LC_{50}$ and the TR of 4th and other instars, respectively, were observed in chlorfenapyr, chlorpyrifos and EPN while higher ones were lufenuron, chlorfluazuron and teflubenzuron. These results mean that insecticides with lower D$LC_{50}$ and DTR are effective in controlling larva of S. litura collected in Milyang, Korea.


  1. Bae, S.D. 1999. Leaf characteristics of leguminous plants and the biology of tobacco cutworm, Spodoptera litura Fabricius, I. The larval development and leaf feeding amount. Kor. J. Appl. Entomol. 38: 217-224
  2. Cho, J.R., W.R. Song, S.Y. Hwang, H.S. Kim and J.O. Lee. 1996. Age-related susceptibility of Spodoptera litura larvae to some insecticides. Kor. J. Appl. Entomol. 35: 249-253
  3. Kim, C.H. and H.Y. Shin. 1987. Studies on bionomics and control of tobacco cutworm, Spdoptera litura Fabricius in southern part of Korea. J. Inst. Agr. Res. Util. Gyeongsang Natl, Univ. 21: 105-122
  4. Minamikawa, H. 1937. Survey on the tobacco cutworm, Spodop tera litura Fabricius. Taiwan Central Res. Inst. Agr. Report 70: 1-66
  5. Mochida, O. and T. Okada. 1974. A bibliography of Spodoptera litura spp. (Lepidoptera: Noctuidae). Misc. Bull. Kyushu Nat. Agr. Expt. Sta. 49: 1-110
  6. Shin, H.Y., C.H. Kim, C.G. Park and Y.S. Lee. 1987. Biology of tobacco cutworm, Spodoptera litura (F.), (Lepidoptera: Noctuidae); I. Seasonal occurrence of tobacco cutworm in southern Korea and larval development, pupal period, adult longevity and oviposition on the different food sources. Res. Rept. RDA (D . M & U) 29: 301-307
  7. Bae, S.D. and H.J. Cho. 1998. Study on the physio-biology and control of Spodoptera litura Fabricius. Pl. Environ. Res. Rept. Nat. Yeongnam Agr. Expt. Stan. RDA. pp. 834-841
  8. Moussa, M., A. Zaher and F. Kotby. 1960. Aboundance of the cotton leafworm, Prodenia litura (F.) (S. litoralis), in relation to host plants, I. host plants and their effect on biology (Lepidoptera: Agrotidae-Zenobiinae). Bull. Soc. Entomol. Egypt 44: 241-251
  9. Aguayo, M.I. and F.R. Villaneueva, F.R. 1985. Susceptibility of Meteorus hyphantriae Riley to emthyl parathion. Southwest. Entomol. 10: 107-109
  10. Raymond, M. 1985. Presentation d'un programme d'analyse logprobit pour micro-ordinateur. Cah. ORSTOM. Ser. Ent. Med. et Parasitol. 22: 117-121
  11. Cho, J.R., Y.J. Kim, H.S. Kim, J.K. Yoo and J.O. Lee. 1999a. Toxicity of insecticides and metabolism of deltamethrin in Helicoverpa assulta Guenee and Spodoptera litura Fabricius. J. Asia-Pacific Entomol. 2: 77-83
  12. Taguchi, R. 1961. On the outbreak of tobacco cutworm, Spodoptera litura Fabricius. Plant Quarantine 15: 541-542
  13. Bae, S.D. and K.B. Park. 1999. Effects of temperature and food source on pupal development, adult longevity and ovipositionof the tobacco cutworm, Spodoptera litura Fabricius. Kor. J. Appl. Entomol. 38: 23-28
  14. Nasr, E.S., M.A. Moussa and A.S. Hassan. 1960. Soil moisture in relation to population and moth emergence of the cotton leaf worm, Prodenia litura Fabricius. Bull. Soc. Entom. Egypte. XLIV: 377-382
  15. Anonymous. 2002. Pesticide guide book. Korea crop protection association 911pp
  16. Kim, Y.G., J.I. Lee, S.Y. Kang and S.C. Han. 1998a. Age variation in insecticide susceptibility and biochemical changes of beet armyworm, Spodoptera exigua (Hubner). J. Asia-Pacific Entomol. I: 109-113
  17. Bae, S.D., K.B. Park and Y.J. Oh. 1997. Effects of temperature and food source on the egg and larval devlopment of tobacco cutworm, Spodoptera litura Fabricius. Kor. J. Appl. Entomol. 36: 48-54
  18. Cho, J.R., Y.J. Kim, K.J. Kim, H.S. Kim, J.K. Yoo and J.O. Lee. 1999b. Electrophoretic pattern of larval esterases in field and laboratory-selected strains of the tobacco cutworm, Spodoptera litura (Fabricius). J. Asia-Pacific Entomol. 2: 39-44
  19. Bratisten, S.B. and R.L. Metcalf. 1973. Age-dependent variations in the response of several species of diptera to insecticidal chemicals. Pestic. Biochem. Physiol. 3: 189-198
  20. Anonymous. 2001. 5-year programs for environment-friendly agriculture. Ministry of Agriculture and Forestry. 159pp
  21. Critchley, B.R. 1972. A laboratory study on the effects of some soil-applied organophosphorus pesticide, thionazin, on predacious carabidae (Coleoptera). Bull. Entomol. Res. 62: 229-241