Serological survey of avian pneumovirus infection in laying hens of Gyeongbuk province

경북지역 산란계에서 avian pneumovirus 에 대한 항체가조사

  • 김순태 (경상북도가축위생시험소) ;
  • 김성국 (경상북도가축위생시험소) ;
  • 조민희 (경상북도가축위생시험소) ;
  • 김영환 (경상북도가축위생시험소)
  • Published : 2003.03.01


Avian pneumoviros(APV), also known as avian rhinotracheitis virus(ARTV), affects both turkeys and chickens and is known to be the primary causative agent of turkey rhinotracheitis (TRT). The aim of this study was to establish the presence or absence of antibodies to avian pneumovirus in the commercial poultry population of Korea. For this purpose, chicken serum samples were obtained and tested by an enzyme-linked immunosorbent assay (ELISA). The tested serum was collected in laying hens with reduction of egg production or normal in Gyeongbuk province. A total of 184 sera representing 42 different poultry farms of the Gyeongbuk region of Korea were included in this study. Laying hens of 16 different farms with reduction of egg production and laying hens of 26 different farms with clinically healthy at the time of serum sampling were considered positive to antibody against APV. In the farms with reduction of egg production, positive farm to antibody against avian pneumovirus were 11 of 16 different farms(68.8%) and positive sera were 47(58.8%) of 80 different serum. In the farms with clinically healthy flock, positive farm to antibody against avian pneumovirus were 12(46.2%) of 26 different farms and positive serum sample were 39(37.5%) of 104 different sera. According to the results tested to 42 different farms in 14 city, 8 of 14 city have flocks with antibody positive laying hens against APV, 1 of 14 city have antibody suspicious and 5 of 14 city shown antibody negative, respectively.



  1. Shin HJ. McComb B, Back A, et al. 2000. Susceptibility of broiler chicks to infection by avian pneumovirus of turkey origin. Avian Dis 44:797-802
  2. Pedersen Jc, Reyonlds DL, Ali A. 2000. The sensitivity and specificity of a reverse transcription polymerase chainreaction assay for the avian pneumovirus (Colorado strain). Avian Dis 44:681-685
  3. Ali A, Reynolds DL. 1999. A reverse transcription polymerase chain reaction assay for the detection of avian pneumovirus(Colorado Strain). Avian Dis 43:600-603
  4. Heckert RA, Myers DJ, Afshar A, et al. 1994. Development and Evaluation an enzyme linked immunosorbent assay for the detection of antibodies to avian pneumovirus. Avian Dis 38:694-700
  5. Hafez HM, Hess M, Prusas C, et al. 2000. Presence of avian pneumovirus type A in continental Europe during the 1980. J Vet Med B 47:629-633
  6. Toro H, Hidalgo H, Ibanez M, et al. 1998. Serological evidence of pneumovirus in chile. Avian Dis 42:815-817
  7. Panigrahy B, Senne DA, Pedersen JC, et al. 2000. Experimental and serologic observations on avian pneumovirus(APV/ turkey/Colorado/97) infection in turkeys. Avian Dis 44:17-22
  8. Shin HJ. 2002. Murine susceptibility to avian pneumovirus(APV) of turkey origin. Korean J Vet Res. 41(4):529-533
  9. Bayon-Auboyer MH, Arnauld C, Toquin D, et al. 2000. Nucleotide sequences of the F, L, and G protein genes of two non-A/non-B avian pneumoviruses(APV) reveal a novel APV subgroup. J Gen Virol 81:2723-2733
  10. Bayon MH, Jestin V, Toquin D, et al. 1999. Comparison of F-, G- and N-based RT-PCR protocols with conventional virological procedures for the detection and typing of turkey rhinotracheitis virus. Arch Virol 144:1091-1109
  11. Mase M, Asahi S, Imai K, et al. 1996. Detection of turkey rhinotracheitis virus from chickens with swollen head syndrome by reverse transcriptasepolymerase chain reaction(RT-PCR). J Vet Med Sci 58(4):359-361
  12. Gough RE, Manvell RJ, Drury SEN, et al. 1994. Isolation of an avian pneumovirus from broiler chickens. Vet Rec 134:353-354
  13. 이윤정. 1995. 단크론성 항체 및 polymerase chain reaction을 이용한 turkey rhinotracheitis virus 검출에 관한 연구. 건국대학교대학원 석사학위논문
  14. 矢澤慈人, 柴田 軌. 2000. 國内のコマーシャ ル採卵鶏農場および 卵用種鶏農場における 七面鳥鼻気管炎ウイルス(TRTウイルス) の 抗体調査. 鶏病研報 36(1):37-39
  15. Gulati BR, Munir S, Patnayak DP, et al. 2001. Detection of antibotics to U.S. isolates of avian pneumovirus by a recombinant neucleocapsid protein-based sandwich enzyme-linked immunosorbent assay. J Clin Microbiol 39(8):2967-2970