Protease antigen recovery induces non-specific reaction in B-Cells

Protease antigen recovery의 B-Cell에 대한 비특이반응 유발

  • Kim, Ok-Jin (USDA-ARS, Animal Disease Research Unit, WSU) ;
  • Yi, Seong-Joon (College of Veterinary Medicine, Kyungpook National University)
  • 김옥진 (미국 농무부 동물질병연구소) ;
  • 이성준 (경북대학교 수의과대학)
  • Published : 2003.06.01

Abstract

Antigen retrieval (AR) techniques were widely used to recover the antigenicity from the fixed tissues, which were guided by the philosophy of rendering immunohistochemistry (IHC) applicable to routine formalin-fixed, paraffin-embedded tissues for wide application of IHC in research and clinical filed for morphological observation like as anatomy, histology and pathology. Protease antigen recovery (PAR) is an AR technique, which is obtained the antigen retrieve by using enzyme digestion, and commonly used in IHC field. However, during the IHC for the detection of ovine herpesvirus 2 (OvHV-2) antigen, we noted lymphocyte-like cells-specific staining in the infiltrated cells into various organs like as liver and kidney, which was also shown in the IHC tissues with isotype control. However, those signals were not observed in the tissues conducted with in situ hybridization. Therefore, we analyzed the specificity of the IHC detection results. We found that PAR may induce false-positive result during IHC in lymphocyte-like cells, which were infiltrated mainly around vessels and in interstitial tissues. Through the Phenotyping, we realized that those false-positive cells were B-cell-related cells. These results suggest that PAR, a AR using protease, may induce non-specific false-positive reactions during IHC.