Antioxidative and Cholesterol-reducing Activity of Bacillus polyfermenticus SCD

Bacillus polyfermenticus SCD의 항산화 및 콜레스테롤 저하효과

  • 정황영 (경남대학교 생명과학부) ;
  • 김태훈 (건국대학교 동물생명과학부) ;
  • 박준석 (건국대학교 동물생명과학부) ;
  • 김기태 (㈜프로코바이오텍) ;
  • 백현동 (건국대학교 동물생명과학부)
  • Published : 2003.10.01

Abstract

Antioxidative and cholesterol-reducing activity of Bacillus polyfermenticus SCD were measured to characterize its probiotic properties. DPPH (1,1-diphenyl-2-picyryl hydrazyl) radical scavenging activity of the culture supernatant of B. polyfermenticus SCD was estimated to be 48%. The culture supernatant on the peroxidation of linoleic acid were investigated and the value was shown to be about 45%. The inhibition of TBA (2-thiobarbituric acid) formation of the culture supernatant was revealed 60% when stimulator was presented. The SOD-like activity of the culture supernatant was about 15%, which is similar to BHT (butylated hydroxytoluene) and ${\alpha}$-tocopherol. After cultured in TSB broth added soluble cholesterol either 0.1% or 0.3% of oxgall in 37$^{\circ}C$ for 24 h aerobically, cholesterol-reducing activities were revealed about 67% or 64%, respectively. To test whether the products are cholesterol-related or not, residual activity was determined. The cholesterol activity was rarely changed. In addition, when the cell extracts recovered after cultivation, was tested in absence of cholesterol, cholesterol activity was not detected. However, cholesterol activity was detected in the presence of cholesterol. Thus, it was assumed that B. polyfermenticus SCD could reduce cholesterol by conjugating with it, rather than by digesting the cholesterol using cholesterol-hydrolyzing enzymes.

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