Antioxidative and Cholesterol-reducing Activity of Bacillus polyfermenticus SCD

Bacillus polyfermenticus SCD의 항산화 및 콜레스테롤 저하효과

  • 정황영 (경남대학교 생명과학부) ;
  • 김태훈 (건국대학교 동물생명과학부) ;
  • 박준석 (건국대학교 동물생명과학부) ;
  • 김기태 (㈜프로코바이오텍) ;
  • 백현동 (건국대학교 동물생명과학부)
  • Published : 2003.10.01


Antioxidative and cholesterol-reducing activity of Bacillus polyfermenticus SCD were measured to characterize its probiotic properties. DPPH (1,1-diphenyl-2-picyryl hydrazyl) radical scavenging activity of the culture supernatant of B. polyfermenticus SCD was estimated to be 48%. The culture supernatant on the peroxidation of linoleic acid were investigated and the value was shown to be about 45%. The inhibition of TBA (2-thiobarbituric acid) formation of the culture supernatant was revealed 60% when stimulator was presented. The SOD-like activity of the culture supernatant was about 15%, which is similar to BHT (butylated hydroxytoluene) and ${\alpha}$-tocopherol. After cultured in TSB broth added soluble cholesterol either 0.1% or 0.3% of oxgall in 37$^{\circ}C$ for 24 h aerobically, cholesterol-reducing activities were revealed about 67% or 64%, respectively. To test whether the products are cholesterol-related or not, residual activity was determined. The cholesterol activity was rarely changed. In addition, when the cell extracts recovered after cultivation, was tested in absence of cholesterol, cholesterol activity was not detected. However, cholesterol activity was detected in the presence of cholesterol. Thus, it was assumed that B. polyfermenticus SCD could reduce cholesterol by conjugating with it, rather than by digesting the cholesterol using cholesterol-hydrolyzing enzymes.


  1. Pharm. Res. v.44 In vitro antioxidant properties of dantrolene sodium Buyukokuroglu,M.E.;I.Gulcin;O.I.Kufrevioglu
  2. Kor. J. Food Sci. Technol. v.28 Effect of plant extracts with superoxide dismutase-like activity in survival of fruit flies under oxidative sress Han,D.S.;J.H.Kwak;S.H.Kim;S.J.Kim
  3. Kor. Nutr. Soc. v.34 Effects of green tea catechin on the superoxide dismutase, glutathione peroxidase and xanthine oxidase activities of kidney in diabetic rats Rhee,S.J.;J.H.Choi
  4. J. Dairy Sci. v.76 Effect of antioxidative lactic acid bacteria on rats fed a diet deficient I vitamin E Hiroe,K.;S.Masahiro;N.Hajime;S.Yutaka
  5. J. Korean Sci. Nutr. v.26 Dietary fiber and cholesterol metabaolism Kang,H.J.;Y.S.Song
  6. Agric. Biol. Chem. v.33 Microbial transformation of sterols: Part I. decomposition of cholesterol by microorganisms Arima,K.;N.Michitaro;B.Moo;T.Gakuzo
  7. Appl. Environ. Microbiol. v.59 The assumed assimilation of cholesterol by lactobacilli and Bifidobacterium bifidum is due to their bile salt-deconjugating activity Klaver,F.A.M.;van der R. Meer
  8. J. Agric. Food Chem. v.47 Antioxidative ability of lactic acid bacteria Lin,M.Y.;C.L.Yen
  9. Appl. Environ. Microbiol. v.65 Characterization of two Bacillus probiotics Green,D.H.;P.R.Wakeley;A.Page;A.Barnes;L.Baccigalupi;E.Ricca;S.M.Cutting
  10. Appl. Environ. Microbiol. v.66 Characterization of Bacillus species used for oral bacteriotherapy and bacterioprophylaxis of gastrointestinal disorders Hoa,N.T.;L.Baccigalupi;A.Huxham;A.Smertenko;P.H.Van;S.Ammendola;E.Ricca;S.M.Cutting
  11. Kor. J. Appl. Microbiol. Biotechnol. v.28 Microbiological identification of medical probiotic Bispan strain Jun,K.D.;K.H.Lee;W.S.Kim;H.D.Paik
  12. J. Agric. Food Chem. v.50 Antioxidative activity and safety of the 50% ethanol extract from red bean fermented Bacillus subtilis IMR-NK1 Chung,Y.C.;C.T.Chang;W.W.Chao;S.T.Chou
  13. Kor. J. Food Sci. Technol. v.31 Fatty acid composition and antioxidative activity in wax corn (Zea mays L.) Fls Seo,Y.H.;I.J.Kim;H.K.Min;S.U.Park
  14. J. Korean Soc. Food Sci. Nutr. v.30 Antioxidant effect of aqueous green tea on soybean oil Park,B.H.;H.K.Choi;H.S.Cho
  15. Kor. J. Food Sci. Nutr. v.30 Antioxidative activity of extracts from fruit of Cudranis tricuspidata Cha,J.Y.;Y.S.Cho
  16. Agric. Biol. Chem. v.55 Purification and characterization of superoxide dismutase from Aerobacter aerogenes Kim,S.W.;S.O.Lee;T.H.Lee
  17. Appl. Environ. Microbiol. v.68 Cheolesterol assimilation by lactic acid bacteria and bifidobacteria isolated from the human gut Dora,I.;A.Pereira;R.G.Glenn
  18. Curr. Microbiol. v.33 Bifidobacteria strain behavior toward cholesterol: coprecitation with bile salt and assimilationm Tahiri,K.;J.P.Grill;F.Schneider
  19. Curr. Microbiol. v.34 Involvement of trihydroxy conjugated bile salts in cholesterol assimilation by bilfidobacteria Tahiri,K.;J.P.Grill;F.Schneider
  20. Appl. Environ. Microbiol. v.49 Assimilation of cholesterol by Lactobacillus acidophilus Gilliland,S.E.;C.R.Nelson;C.Maxwell