Cellular Force Sensing for Force Feedback-Based Biological Cell Injection

힘 피드백 기반의 세포조작을 위한 세포막 침습력 측정

  • Kim, Deok-Ho ;
  • Yun, Seok ;
  • Kang, Hyun-Jae ;
  • Kim, Byung-Kyu
  • 김덕호 (한국과학기술연구원 마이크로시스템센터) ;
  • 윤석 (한국과학기술연구원 마이크로시스템센터) ;
  • 강현재 (한국과학기술연구원 마이크로시스템센터) ;
  • 김병규 (한국과학기술연구원)
  • Published : 2003.12.01


In biological cell manipulation, manual thrust or penetration of an injection pipette into an embryo cell is currently performed by a skilled operator, relying on visual feedback information only. Accurately measuring cellular forces is a requirement for minimally invasive cell injections. Moreover, the cellular force sensing is essential in investigating the biophysical properties for cell injury and membrane modeling studies. This paper presents cellular force measurements for the force feedback-based biomanipulation. Cellular force measurement system using piezoelectric polymer sensor is implemented to measure the penetration force of a zebrafish egg cell. First, measurement system setup and calibration are described. Second, the force feedback-based biomanipulation is experimentally carried out. Experimental results show that it successfully supplies real-time cellular force feedback to the operator at tens of uN and thus plays a main role in improving the reliability of biological cell injection tasks.


Cellular Force Sensing;Force Feedback;Piezoelectric Polymer;Biomanipulation;Egg Cell


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