Erythrinae Cortex inhibits Synthesis of Inflammatory Cytokines induced by IL-1$\beta$ and TNF-$\alpha$ in Cultured Human Synovial Cells

  • Lee Ho (Department of Pathology, Oriental Medical Collage, Daejeon University) ;
  • Kim Dong Hee (Department of Pathology, Oriental Medical Collage, Daejeon University)
  • Published : 2003.08.01

Abstract

Our study shows that EC extract has inhibitory effect on pro-inflammatory cytokines such as TNF-α, IL-1, IL-6, iNOS and COX2 in hFLSs. IL-1β, IL-6, iNOS and COX2 mRNA expression is suppressed at a low dasage (1㎍/ml) of EC extract. TNF-α was also suppressed at higher dosages (10 ㎍/ml, 100㎍/ml). EC extract also inhibited TNF-α, IL-1β and IL-6 production in pro-inflammatory cytokine stimulated-hFLSs. Expecially IL-1β(p<0.05) production are suppressed significantly. On the other hand, EC extract did not show any cytotoxicity. Thses data suggest that EC extract has anti-inflammatory effect mostly by inhibiting IL-1β production, and thus could be used to prevent or treat some inflammatory disease such as RA. It remains to be known what are the major components responsible for anti-inflammatory effect and what is the main mechanism.

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