Determination of Sildenafil and Its Related Substances Using High Performance Thin Layer Chromatography

고성능 박층 크로마토그래프를 이용한 실데나필과 그 유사 물질의 정량에 관한 연구

  • Received : 2002.11.11
  • Accepted : 2003.01.17
  • Published : 2003.02.25

Abstract

The rapid and sensitive determination method of sildenafil and its related compounds (Vardenafil, Homosildenafil, Tadanafil.) has been investigated using high performance thin layer choromatography (HPTLC). Optimizing separation conditions and simultaneous determination method of these compounds were studied. The calibration curves of those compounds at 254 nm was found to be linear in the range of $1.0{\sim}56.5{\mu}g/mL$, respectively. The detection limts (LOD) and quantification limits (LOQ) of these were found to be $0.8{\sim}1.8{\mu}g/mL$ and $1.0{\sim}2.3{\mu}g/mL$. The coefficient of variation (C.V.) were less than 2.5%. Finally, the present method was applied to determine sildenafil and its related substances in dietary supplement.

Keywords

Sildenafil;HPTLC;Dietary supplement

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