Simultaneous Determination of 4-Nitrotoluene and Benzophenone in Ground Water and Soil by Gas Chromatography-Mass Spectroscopy

  • Kwon, Oh-Seung (Toxicology Lab., Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology) ;
  • Kim, Eun-Young (Toxicology Lab., Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology) ;
  • Ryu, Jae-Chun (Toxicology Lab., Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology)
  • Received : 2002.06.01
  • Accepted : 2003.01.08
  • Published : 2003.02.25


Benzophenone (BZP) and 4-nitrotoluene (4-NT) listed as endocrine disrupting chemicals are suspected to contaminate ground water sites and soil. Analytical method for simultaneous determination of the two chemicals in soil and ground water was developed by gas chromatography-mass spectrometry. Water (100 ml) was extracted with hexane, and soil (10 g) was extracted with methanol and hexane. Recovery in water was >72% for BZP and 90-118% for 4-NT. Recovery for 4-NT and BZP in soil was 51-59% with coefficient variation of less than 19.5%. Calibration curves showed a good linearity with $r^2=0.997$. In water and soil collected at nation-wide sites, BZP was found at 5 sites among 43 water sites at the concentration of $14.87{\pm}3.83ng/100 ml$. No 4-NT was found. It is suggested that this method is appropriate to the simultaneous quantitation of 4-NT and BZP in ground water and soil samples.


  1. Appl. Environ. Microbiol. v.64 no.2 T. Spiess;F. Desiere;P. Fischer;J. C. Spain;H. J. Knackmuss;H. Lenke
  2. Journal of Chromatography B Biomedical Sciences and Applications v.710 no.1-2 M. Bader;T. Goen;J. Muller;J. Angerer.
  3. J. Biol. Chem. v.268 no.19 M. E. Salvucci;K. Rajagopalan;G. Sievert;B. E. Haley;D. S. Watt
  4. Biol. Med. v.23 no.7 A. K. Krainev;R. I. Viner;D. J. Bigelow;D. J. Free Radic
  5. Journal of Biological Chemistry v.275 no.8 Probing subunit interactions in α class rat liver glutathione S-transferase with the photoaffinity label glutathionyl S-[4-(succinimidyl)benzophenone] J. Wang;S. Bauman;R. F. Colman
  6. Food. Addit. Contam. v.14 no.1 L. Castle;C. P. Ofen;M. J. Baxter;J. Gilbert
  7. J. Anal. Toxicol. v.23 no.1 F. Guan;H. Seno;A. Ishii;K. Watanabe;T. Kumazawa;H. Hattori;O. Suzuki
  8. International Journal of Pharmaceutics v.189 no.2 Quantitative HPLC analysis of sunscreens and caffeine during in vitro percutaneous penetration studies. G. Potard;C. Laugel;A. Baillet;H. Schaefer;J. P. Mart
  9. Journal of Chromatography A v.832 no.1-2 V. Vanquer;C. Rodriguez;C. Coiffard;L. J. Coiffard;Y. De. Roeck-Holtzhauer
  10. Analytical methods for endocrine disruptors National Institute for Environmental Studies(NIES)
  11. Environmental Health Perspectives v.103 no.6 A variety of environmentally persistent chemicals, including some phthalate plasticizers, are weakly estrogenic. S. Jobling;T. Reynolds;R. White;M. G. Parker;J. P. Sumpter
  12. Br. J. Ind. Med. v.43 no.7 B. H. Woolen;M. G. Hall;R. Craig;G. T. Steel
  13. Laboratory manual physical-chemical method #3550B (SW-846 Update Ⅲ, Revision 2) Ultrasonic extraction, Test methods for evaluating solid waste U. S. Environmental Protection Agency