Gas Chromatographic Analysis on the Residual of Fungicide Fenhexamid in Crops(Cucumber, Strawberry and Grape)

작물(오이, 딸기, 포도) 중 살균제 Fenhexamid의 잔류성에 대한 기체 크로마토그래피 분석

  • Han, Seong-Soo (Department of Agricultural Chemistry, Wonkwang University) ;
  • Lo, Seog-Cho (Department of Agricultural Chemistry, Wonkwang University) ;
  • Kim, Won-Ju (Department of Agricultural Chemistry, Wonkwang University) ;
  • Park, PiII-Jae (Department of Agricultural Chemistry, Wonkwang University) ;
  • Kim, Il-Kwang (Department of Chemistry, Wonkwang University)
  • 한성수 (원광대학교 생명자원과학대학 농화학과) ;
  • 노석초 (원광대학교 생명자원과학대학 농화학과) ;
  • 김원주 (원광대학교 생명자원과학대학 농화학과) ;
  • 박필재 (원광대학교 생명자원과학대학 농화학과) ;
  • 김일광 (원광대학교 자연과학대학 화학과)
  • Received : 2002.08.21
  • Accepted : 2002.12.20
  • Published : 2003.02.25

Abstract

The optimum conditions for the residue analysis of hydroxyanilide fungicide fenhexamid on cucumber, strawberry and grape were investigated by gas chromatography equipped with electron capture detector (GC-ECD) and the residual amount was determined by sprayed days before harvest. Each samples were extracted with acetone, filtered and concentrated to 50 mL. The concentrated extracts were transferred to dichloromethane and then thoroughly concentrated. The concentrated phase was loaded on the filtration column stuffed with silica gel and purified with acetone:hexane (5:95, 15:85, v/v) mixed solvent. The regression equation and linearity of the standard calibration curves between 0.05~2.00 ng were as follows : cucumber; Y=312.40X+10.26, $R^2=0.9996$, strawberry; Y=313.33X+5.54, $R^2=0.9998$, grape; Y=253.27X-2.23, $R^2=0.9994$. From the standard additional experiments with 0.10 mg/L and 0.40 mg/L, the average recoveries of cucumber, strawberry and grape were 94.8%, 88.1% and 93.7%, respectively and the detection limits were all the same as 0.01 mg/L. Residual amounts in crops were ranged from 0.01 to 0.58 mg/L.

Keywords

fenhexamid;residue analysis;cucumber;strawberry;grape

Acknowledgement

Supported by : 원광대학교, 주식회사 미성

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