A study on the optimal HPLC condition for peptides complex analysis using mass spectrometry

질량분석기를 단백질 분석에 적용하기 위한 고성능액체크로마토그래피 최적조건 연구

  • 권성원 (텍사스 주립대 사우스웨스턴 메디컬 센터) ;
  • 박철홍 (바임 래버러토리즈)
  • Received : 2002.06.10
  • Accepted : 2002.12.03
  • Published : 2003.02.25

Abstract

Peptides separation in high performance liquid chromatography (HPLC) is very important for the analysis of total proteins using mass spectrometry rather than two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). In this study, we investigated the optimal HPLC condition of peptides for the use of mass fingerprinting. As a result of pursuing a combination of solvent additives for HPLC, water and acetonitile containing both 0.1% trifluoroacetic acid and 0.1% acetic acid respectively showed the most efficient resolution and sensitivity.

Keywords

liquid chromatography;mass spectrometry;peptides;proteomics

Acknowledgement

Supported by : 한국과학재단

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