A Comparison of Sonication and Microwave-assisted Extraction Method for Speciation of Arsenic in Fish Tissue, DORM-2

어류중 비소의 종분화 분석을 위한 초음파 추출법과 마이크로파 추출법의 비교

  • Yoon, Cheol-Ho (Hazardous Substance Research Team, Korea Basic Science Institute) ;
  • Park, Yong-Chul (Department of Oceanography, Inha University) ;
  • Hong, Jong-Ki (Hazardous Substance Research Team, Korea Basic Science Institute)
  • 윤철호 (한국기초과학지원연구원 유해물질분석연구팀) ;
  • 박용철 (인하대학교 해양학과) ;
  • 홍종기 (한국기초과학지원연구원 유해물질분석연구팀)
  • Received : 2003.01.20
  • Accepted : 2003.02.20
  • Published : 2003.04.25

Abstract

Comparison of a microwave-assisted extraction with sonication extraction was performed for arsenic speciation in fish tissue with chromatographic separation and inductively coupled plasma mass spectrometry detection. The detection limits of arsenicals with ultrasonic nebulizerand cross-flow nebulizer were shown to be similar. The arsenicals investigated were arsenobetaine (AsB), arsenite [As(III)], dimethylarsine acid (DMA), monomethylarsonic acid (MMA), arsenate [As(v)], and phenylarsonic acid (PAA). Quantitative extraction of arsenicals from dogfish muscle, DORM-2, standard reference material of NRCC (National Research Council of Canada) was achieved using 50% (v/v) methanol-water in both extraction methods. Extraction efficiency of arsenobetaine in both methods is greater than 82% with RSDs on replicates of less than 5%. The concentrations of AsB determined in extract of microwave assisted extraction and sonication methods were $14.18{\pm}0.42mg\;kg^{-1}$ and $13.54 {\pm}0.84mg\;kg^{-1}$, respectively. And the concentrations of DMA were $0.45{\pm}0.06mg\;kg^{-1}$ and $0.44{\pm}0.06mg\;kg^{-1}$, respectively.

Keywords

Arsenic;speciation;DORM-2;HPLC;ICP-MS

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