Comparisons of sample preparation (acid digestion and microwave digestion) and measurement (inductively coupled plasma mass spectrometry and graphite furnace atomic absorption spectrometry) in the determination of bone lead

골중납 측정의 시료 전처리 (산분해법과 마이크로웨이브 분해법)와 측정 방법 (유도결합 플라즈마 질량분석법과 흑연로 원자 흡수 분광 광도법)의 비교

  • Yoon, Chungsik (Department of Occupational Health, Catholic University of Daegu) ;
  • Choi, Inja (Wonjin Institute for Occupational & Environmental Health) ;
  • Park, Sungkyun (Department of Environmental Health, Harvard School of Public Health) ;
  • Kim, Rokho (Department of Environmental Health, Harvard School of Public Health)
  • 윤충식 (대구가톨릭대학교 산업보건전공) ;
  • 최인자 (원진노동환경건강연구소) ;
  • 박성균 (하버드대학교 보건대학원 환경보건학과) ;
  • 김록호 (하버드대학교 보건대학원 환경보건학과)
  • Received : 2003.02.14
  • Accepted : 2003.03.18
  • Published : 2003.04.25

Abstract

This study was conducted to evaluate two sample digestion procedures and instrumental determination parameters for analysis of lead in bone. Amputated human legs were treated by acid digestion or microwave dissolution prior to spectrometric analysis. Inductively coupled plasma mass spectrometry (ICP-MS) and graphite furnace atomic absorption spectrometry (GF-AAS) were used for determining bone lead levels. Recovery efficiencies using standard reference material from acid digestion measured by ICP-MS were in good agreement with those of the certified value, but in cases of acid digestion by GF-AAS and microwave digestion by both two methods, recovery underestimated and overestimated, respectively. For the bone samples, the lead concentrations obtained by ICP-MS after acid digestionwere in good agreement with those by GF-AAS (correlation coefficient = 0.983), but GF-AAS gave systematically higher values than ICP-MS. While a good agreement between two analytical methods after microwave digestion was also obtained (correlation coefficient = 0.950), bone lead concentrations from microwave were relatively higher than those from acid digestion. In conclusion, the use of the simple nitric acid digestion procedure at an ambient temperature coupled to ICP-MS seems to be efficient for the determination of lead in bone in consideration for both the convenience and validity.

Keywords

bone lead;acid digestion;microwave oven;SRM;ICP-MS;GF-AAS

Acknowledgement

Supported by : Daegu Catholic University

References

  1. Introduction to microwave samples preparation Manual and robotically controlled microwave pressure dissolution of minerals J. M. Labrecque;H. M. Kinston(Ed.);L. B. Jassie(Ed.)
  2. Clinica Chimica Acta v.293 no.1-2 J. Scancar;R. Milacic;M. Benedik;P. Bukovec https://doi.org/10.1016/S0009-8981(99)00239-9
  3. Sci. Total Environ v.159 no.2;3 I. Baranowska;K. Czemicki;R. Aleksandrowicz
  4. The Science of The Total Environment v.64 no.3 Lead in human bones. Investigations on an occupationally non-exposed population in Southern Bavaria (F.R.G.) I. Adults G. A. Drasch;J. Bhn;C. Baur https://doi.org/10.1016/0048-9697(87)90252-X
  5. Archives of Environmental Contamination and Toxicology v.24 no.4 Bone-lead analysis: development of analytical methodology for milligram samples. K. S. Subramanian;J. W. Connor;J. C. Meranger https://doi.org/10.1007/BF01146168
  6. Science of The Total Environment v.162 no.2 Trace elements in ribs of elderly people and elemental variation in the presence of chronic diseases J. Yoshinaga;T. Suzuki;M. Masatoshi;M. Hayakawa https://doi.org/10.1016/0048-9697(95)04470-L
  7. Analytical Science & Technology v.15 no.4 C.S. Yoon;I.J. Choi;T.S. Kang;W.S. Yang;D.W. Park;D.Y. Park
  8. J. AOAC Int v.81 no.6 P. H. Siitonen;H. C. Thompson, Jr
  9. Arch Environ Health v.43 L. E. Wittmers;J. Wallgren;A. C. Aufderheide;G. Rapp
  10. Journal of Analytical Atomic Spectrometry v.11 no.1 Y. Y. Zong;P. J. Parsons;W. Slavin https://doi.org/10.1039/ja9961100025
  11. Environ. Health Perspect. v.106 no.SUP.4 H. Hu
  12. Environmental Health Criteria No. 3. Lead International Programme on Chemical Safety
  13. Clinica Chimica Acta v.221 no.1-2 F. Gil;M. L. Prez;A. Facio;E. Villanueva;R. Tojo;A. Gil https://doi.org/10.1016/0009-8981(93)90019-Z
  14. Medical Physics v.27 no.1 Validation of K x-ray fluorescence bone lead measurements by inductively coupled plasma mass spectrometry in cadaver legs. A. Aro;C. Amarasiriwardena;M-L. Lee;R. Kim;H. Hu https://doi.org/10.1118/1.598863
  15. Physics in Medicine and Biology v.40 no.9 K X-ray fluorescence measurements of bone lead concentration: the analysis of low-level data R. Kim;A. Aro;A. Rotnitzky;C. Amarasiriwardena;H. Hu https://doi.org/10.1088/0031-9155/40/9/007
  16. The Science of The Total Environment v.255 no.1-3 H.-W. Kuo;S.-M. Kuo;C.-H. Chou;T.-C. Lee https://doi.org/10.1016/S0048-9697(00)00448-4