Separation and purification of substance having matrix metalloproteinase-9 inhibition effect in Ulmus davidiana Plancn. var. japonica Nakai

유근피(楡根皮)에 존재하는 matrix metalloproteinase-9 억제 물질의 분리 및 정제

  • 한기정 (중앙대학교 자연과학대학 화학과) ;
  • 이광수 (중앙대학교 자연과학대학 화학과) ;
  • 공광훈 (중앙대학교 자연과학대학 화학과) ;
  • 조성희 (중앙대학교 자연과학대학 화학과)
  • Received : 2002.06.17
  • Accepted : 2003.05.27
  • Published : 2003.06.25


Root bark of Ulmus davidiana Plancn. var. japonica Nakai was extracted by using several solvents with different polarities. Each extract was treated on the MMPs obtained from SK-Hep-1 in order to investigate inhibition effect. Zymography of MMPs showed that MeOH extract has significant inhibition effect. On the GC-MS analysis the highest mass to charge ratio (m/z) of the purified substance was 281. Also, on zymography of MMPs the substance showed 47% inhibition effect at the concentration of $314.7{\mu}g/g$. Cell viability of SK-Hep-1 was 60% at $31.47{\mu}g/g$.



Supported by : 중앙대학교


  1. FASEB J. v.8 R. Raghow
  2. Am. J. Respir. Cell Mol. Biol. v.7 G. Murphy;A. J. Docherty
  3. Am. J. Respir. Cell Mol. Biol. v.20 S. D. Shapiro;R. M. Senior
  4. The International Journal of Biochemistry & Cell Biology v.33 no.10 Human endothelial gelatinases and angiogenesis M. Nguyen;J. Arkell;C. J. Jackson
  5. Journal of Investigative Dermatology v.115 no.3 Matrix metalloproteinases in human melanoma. U. B. Hofmann;J. R. Westphal;G. N. P. van Muijen;D. J. Ruiter
  6. BioEssays v.14 no.7 L. M. Matrisian
  7. Journal of Biological Chemistry v.270 no.39 Identification of the Second Membrane-type Matrix Metalloproteinase (MT-MMP-2) Gene from a Human Placenta cDNA Library: Mt-Mmps Form a Unique Membrane-type Subclass in the MMP Family T. Takino;H. Sato;A. Shinagawa;M. Seiki
  8. Journal of Allergy and Clinical Immunology v.104 no.2 Inhaled corticosteroids decrease subepithelial collagen deposition by modulation of the balance between matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 expression in asthma G. Mautino;F. Capony;J. Bousquet;A. M. Bignola
  9. Cancer Chemotherapy and Pharmacology v.43 no.suppl Matrix metalloproteinases and metastasis D. E. Kleiner;W. G. Stetler Stevenson
  10. Trends in Immunology v.22 no.10 Gelatinase B: a tuner and amplifier of immune functions G. Opdenakker;P. E. Van den Steen;J Van Damme
  11. Expert Opinion on Therapeutic Patents v.8 no.3 R. P. Beckett;M. Whittaker
  12. J. Food Sci. Nutr. v.6 no.4 G. H. Lee;C. J. Shin;Y. I. Chang;S. H. Choi;H. R. Oh;M. H. Oh
  13. Analytical Biochemistry v.102 no.1 Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates. C. Heussen;E. B. Dowdle
  14. Journal of Immunological Methods v.89 no.2 Rapid colorimetric assay for cell growth and survival - Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability F. Denizot;R. Lang