Development of microarrayer for manufacturing DNA chip used in genome project

유전자 검색을 위한 DNA 칩 제작용 microarrayer의 개발

  • Lee, Hyun-Dong (Division of Agricultural Engineering, Chungnam Nat'l Univ.) ;
  • Kim, Ki-Dae (Dept. of Automation System, Doowon Technical College) ;
  • Kim, Chan-Soo (Division of Agricultural Engineering, Chungnam Nat'l Univ.) ;
  • Lim, Yong-Pyo (Division of Agricultural Engineering, Chungnam Nat'l Univ.) ;
  • Park, Jung-Kyu (Division of Agricultural Engineering, Chungnam Nat'l Univ.)
  • 이현동 (충남대학교 농업생명과학대학 농업공학부) ;
  • 김기대 (두원공과대학 자동화시스템과) ;
  • 김찬수 (충남대학교 농업생명과학대학 농업공학부) ;
  • 임용표 (충남대학교 농업생명과학대학 농업공학부) ;
  • 박정규 (충남대학교 농업생명과학대학 농업공학부)
  • Published : 2003.06.30

Abstract

This study exploits the robot system which is necessary in gene study, bio-technology industry. As well, it can achieve the job of DNA chip manufacturing whose use rate has been increased recently. The robot consists of DNA spotting device for spotting DNA on the silylated slide and well plate, bed for fixing well-plate, washing & drying device of washing and drying the pin part of DNA spotting device, distillation-water vessel, and discharge vessel of wash water. We made the term of sticking DNA to the pin on well plate to be 15 seconds. The spot size of DNA was set to be 0.28 mm on the average by bringing the slide into contact with pin for 1 second. At this rate, if DNA is spotted in the minimum space possible of about 0.32mm, it can stick about 8,100 DNA spots on the well plate. Analyzing the procedure: Movement starts. Pin washes, dries, and smears DNA on the well plate. Spots DNA onto 12 chips takes 2 minutes and 50 seconds.

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