Modification of Radiation Response in Mice by Dongchongxiacao(Paecilomyces japonica)

고선량 및 저선량 방사선 조사 마우스에서 누에동충하초(Paecilomyces japonica)의 효과

  • Kim, Se-ra (College of Veterinary Medicine, Chonnam National University) ;
  • Oh, Heon (College of Veterinary Medicine, Chonnam National University) ;
  • Lee, Hae-june (College of Veterinary Medicine, Chonnam National University) ;
  • Shin, Dong-ho (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Jong-choon (College of Veterinary Medicine, Chonnam National University) ;
  • Park, In-chul (College of Veterinary Medicine, Chonnam National University) ;
  • Oh, Ki-seok (College of Veterinary Medicine, Chonnam National University) ;
  • Jo, Sung-kee (Korea Atomic Energy Research Institute) ;
  • Kim, Sung-ho (College of Veterinary Medicine, Chonnam National University)
  • Accepted : 2003.05.23
  • Published : 2003.06.30


Cordyceps has a reputation for its broad biological activities and as a tonic which replenishs vital function in Chinese traditional medicines. As an attempt to obtain fundamental data for the development of new type Cordyceps, the effects of the fruiting bodies of cultivated fungus of Paecilomyces japonica grown on silkworm larvae on radiationinduced damages were investigated. We performed this study to determine the effect of Dongchongxiacao (Paecilomyces japonica) on jejunal crypt survival, endogenous spleen colony formation, and apoptosis in jejunal crypt cells and hair follicles of mice irradiated with high and low dose of gamma-radiation. Treatment with Dongchongxiacao showed no significant modifying effects on the jejunal crypt survival and endogenous spleen colony formation. The frequency of radiationinduced apoptosis was reduced by pretreatment of Dongchongxiacao (i.p.: 50 mg/kg of body weight, at 12 and 36 hours before irradiation, p<0.01). The spontaneous levels of apoptotic cells are $0.082{\pm}0.041$ in intestinal crypts and $0.231{\pm}0.084$ per hair follicle section of skin. Pretreatment of Dongchongxiacao was associated with decreases of 26.86% in intestinal crypt and 66.36% in hair follicle decrease in the number of cells with nuclei positively stained for apoptosis compared with the irradiation control group. We demonstrated for the first time that Dongchongxiacao administration could reduce the extent of apoptosis produced by radiation in the hair follicle. The results presented herein that Dongchongxiacao given before irradiation is capable of reducing the severity of cell loss as a result of apoptosis.


Supported by : 과학기술부


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