Modification of Radiation Response in Mice by Dongchongxiacao(Paecilomyces japonica)

고선량 및 저선량 방사선 조사 마우스에서 누에동충하초(Paecilomyces japonica)의 효과

  • Kim, Se-ra (College of Veterinary Medicine, Chonnam National University) ;
  • Oh, Heon (College of Veterinary Medicine, Chonnam National University) ;
  • Lee, Hae-june (College of Veterinary Medicine, Chonnam National University) ;
  • Shin, Dong-ho (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Jong-choon (College of Veterinary Medicine, Chonnam National University) ;
  • Park, In-chul (College of Veterinary Medicine, Chonnam National University) ;
  • Oh, Ki-seok (College of Veterinary Medicine, Chonnam National University) ;
  • Jo, Sung-kee (Korea Atomic Energy Research Institute) ;
  • Kim, Sung-ho (College of Veterinary Medicine, Chonnam National University)
  • Accepted : 2003.05.23
  • Published : 2003.06.30

Abstract

Cordyceps has a reputation for its broad biological activities and as a tonic which replenishs vital function in Chinese traditional medicines. As an attempt to obtain fundamental data for the development of new type Cordyceps, the effects of the fruiting bodies of cultivated fungus of Paecilomyces japonica grown on silkworm larvae on radiationinduced damages were investigated. We performed this study to determine the effect of Dongchongxiacao (Paecilomyces japonica) on jejunal crypt survival, endogenous spleen colony formation, and apoptosis in jejunal crypt cells and hair follicles of mice irradiated with high and low dose of gamma-radiation. Treatment with Dongchongxiacao showed no significant modifying effects on the jejunal crypt survival and endogenous spleen colony formation. The frequency of radiationinduced apoptosis was reduced by pretreatment of Dongchongxiacao (i.p.: 50 mg/kg of body weight, at 12 and 36 hours before irradiation, p<0.01). The spontaneous levels of apoptotic cells are $0.082{\pm}0.041$ in intestinal crypts and $0.231{\pm}0.084$ per hair follicle section of skin. Pretreatment of Dongchongxiacao was associated with decreases of 26.86% in intestinal crypt and 66.36% in hair follicle decrease in the number of cells with nuclei positively stained for apoptosis compared with the irradiation control group. We demonstrated for the first time that Dongchongxiacao administration could reduce the extent of apoptosis produced by radiation in the hair follicle. The results presented herein that Dongchongxiacao given before irradiation is capable of reducing the severity of cell loss as a result of apoptosis.

Acknowledgement

Supported by : 과학기술부

References

  1. 성재모. 한국의 동충하초. 교학사, 서울: 1996
  2. Bao, Z. D., Wu, Z. G. and Zheng, F. Amelioration of aminoglycoside nephrotoxicity by Cordyceps sinensis in old patient. Chin. J. Integr. Med. 1994, 14, 271-273
  3. Chen, G. Z. and Chen, G. L. Effects of Cordyceps sinensis on murine T lymphocyte subsets. Chin. Med. J. 1991, 104, 4-8
  4. Colecchia, M., Frigo, B., Del Boca, C., Guardamagna, A., Zucchi, A., Colloi, D. and Leopardi, O. Detection of apoptosis by the tunel technique in clinically localised prostatic cancer before and after combined endocrine therapy. J. Clin. Pathol. 1997, 30, 384-388
  5. Dewit, L. and Oussoren, Y. Late effects in the mouse small intestine after a clinically relevant multifractioned radiation treatment. Radiat. Res. 1987, 110, 372-384
  6. Ijiri, K. and Potten, C. S. Radiation-hypersensitive cells in small intestine crypts; their relationship to clonogenic cells. Br. J. Cancer. 1986, 53, 20-22
  7. Kiho, T., Hui, J., Yamane, A. and Ukai, S. Poly-saccharides in fungi. XXXII. Hypoglycemic activity and chemical properties of a polysaccharide from the cultural mycelium of Cordyceps sinensis. Biol. Pharm. Bull. 1993, 16, 1291-1293
  8. Liang, Y. L., Liu, Y., Yang, J. W. and Lhi, C. X. Studies on pharmacological activities of cultivated Cordyceps sinensis. Phytotherapy Res. 1997, 11, 237
  9. Okada, S. Radiation-induced cell death, In Altman KI, Gerber GG, Okada S, ed Radiation Biochemistry, Academic Press, New York. 1970, 247-307
  10. Potten, C. S. Hair cortical cell counts (HCCC), a new sensitive in vivo assay with possible applications for biological dosimetry. Int. J, Radiat. Biol. 1993, 63, 91-95
  11. Hale, A. J., Smith, C. A., Sutherland, L. C., Stoneman, V. E., Longthorne, V. L., Culhane, A. C. and Williams, G. T. Apoptosis: molecular regulation of cell death. Eur. J. Biochem. 1996, 236, 1-26
  12. Hsu, H. Y., Hau, D. M. and Lin, C. C. Effects of kuei-pi-tang on cellular immunocompetence of gamma-irradiated mice. Am. J. Chin. Med. 1993, 21, 151-158
  13. 심진영, 이연실, 임순성, 신국현, 현진이, 김성연, 이은방. 눈꽃 동충하초의 약물활성. 생약학회지. 2000, 31, 163-167
  14. Kuo, Y. C., lin, C. Y., Tsai, W. J., Wu, C. L., Chen, C. F. and Shiao, M. S. Growth inhibitors against tumor cells in Cordyceps sinensis other than cordycepin and polysaccharides. Cancer Invest. 1994, 12, 611-615
  15. Lu, G., Yana, M, Shen, Y. and Meng, J. The absorption of Fe, Zn, Cu in siwu, sijunzi, and Liuwei dihuang decoction by snaIl intestine in rals. Qaing Kuo Qmng YaoTsa Chih. 1991, 16, 297-298
  16. Miyanomae, T. and Frindel, E. Radioprotection of hemopoiesis ccnferred by Acanthopanax senticosus Harms (Shigoka) administered before or after irradiation. Exp. Hematol. 1988, 16, 801-806
  17. Muller, W. U. and Streffer, C. Biological indicators for radiation damage, Int. J. Radiat. Biol. 1991, 59, 863-873
  18. 한대석, 송효남, 김영언. 동충하초의 현황, 문제점 및 연구방향. 식품과학과 산업. 1999, 32, 67-71
  19. Kiho, T., Yamane, A., Hui, J., Usui, S. and Ukai, S. Polysaccharides in fungi. XXXVI. Hypoglycemic activity of a polysaccharide (CS-F30) from the cultural mycelium of Cordyceps sinensis and its effect on glucose metabolism in mouse liver. Biol. Pharm. Bull. 1996, 19, 294-296
  20. 한대석, 송효남, 김상희. 동충하초: 새로운 기능성 식품 소재. 식품과학과 산업. 1999, 32, 56-63
  21. Manabe, N., Sugimoto, M., Azuina, Y., Taketomo, N., Yamashita, A., Tsuboi, H., Tsunoo, A., Kinjo, N., Nian-Lai, H, and Miyamoto, H. Effects of the mycelial extract of cultured Cordyceps sinensis on in vivo hepatic energy metabolism in the mouse. Jpn. J. Pharmacol. 1996, 70, 85-88
  22. Kneifel, H., Konig, W. A., Loeffler, W. and Muller, R. Ophiocordin, an antifungal antibiotics of Cordyceps ophioglossoides. Arch. Microbiol. 1977, 113, 121-130
  23. Orazi, A., Du, X., Yang, Z., Kashai, M. and Williams, D. A. Interleukin-ll prevents apoptosis and accelerates recovery of small intestine mucosa in mice treated with combined chemotherapy and radiation. Lab. Invest. 1996, 75, 33-42
  24. Kim, S. H., Jeong, K. S., Ryu, S. Y. and Kim, T. H. Panax ginseng prevents apoptosis in hair follicles and accelerates recovery of hair medullary cell in irradiated mice. In Vivo. 1998, 12, 219-222
  25. Cairnie, A. R Adverse effect of radioprotector WR2721. Radiat. Res. 1983, 94, 221-226
  26. Kuo, Y. C., Tsai, W. J., Shiao, M. S., Chen, C. F. and Lin, C. Y. Cordyceps sinensis as an immunomodulatory agent. Am. J. Chin. Msd. 1996, 24, 111-125
  27. Kobayashi, Y. and Shimizu, D. Iconography of vegetable wasps and plant worms. Hoikusha Pub. Co. Ltd., Osaka. 1983, 280
  28. Ohmori, T., Tamura, K., Fukui, K., Kawanishi, G., Mitsuyama, M., Nomoto, K. and Miyazaki, T. Isolation of galactosaminoglycan moiety (CO-N) from protein-bound polysaccharide of Cordyceps ophioglossoides and its effects against murine tumors. Chem. Pharm. Bull (Tokyo). 1989, 37, 1019-1022
  29. Orazi, A., Du, X. and Yang, Z. Interleukin-l1 prevents apoptosis and accelerates recovery of small intestinal mucosa in mice treated with combined chemotherapy and radiation. Lab. Invest. 1996, 75, 33-42
  30. Ledda-Columbano, G. M., Coni, P., Curto, M., Giacomini, L., Faa, G., Oliverio, S., Piacentini, M. and Columbano, A. Induction to two different modes of cell death, apoptosis and necrosis, in rat liver after a single dose of thioacetamide. Am. J. Pathol. 1991, 139, 1099-1109
  31. Cunningham, K. G., Manson, W., Spring, E. S. and Hutchison, A. A. Cordycepin, a metabolic product isolated from cultures of Cordyceps militaris (Linn.). Link Nature. 1950, 166, 9
  32. Geng, L. and Potten, C. S. Changes after irradiation in the number of mitotic cells and apoptotic fragments in growing mouse hair follicles and in the width of their hairs. Radiat. Res. 1990, 123, 75-81
  33. Guan, Y. J., Hu, Z. and Hou, M. Effect of Cordyceps sinensis on T-lymphocyte subsets in chronic renal failure. Chin. J. Integr. Med. 1992, 12, 338-339
  34. Liu, Y., Wu, C. and Li, C. Antioxidation of Paesilomyces sinensis (S. pnov.). Chin. Med. J. 1991, 16, 240-242
  35. Milas, L., Hunter, N., Ito, H. and Peters, A. J. In vivo radioprotective activities of diethydithiocarbamate. Int. J. Radiat. Oncol. Biol. Phys. 1984, 10, 2335-2343