Effect on Field Trial of Oriental Herbal Medicine Feed Additives on Prevention of Salmonella in Chickens

Salmonella 방제에 대한 한방사료첨가제의 야외적용 효과

  • Kang, Ho-jo (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Young-hwan (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Hu-jang (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Jong-shu (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Toh-gyong (Kyongnam Livestock Promotion Institute) ;
  • Kim, Eun-hee (Chinju International University) ;
  • Park, Mi-rim (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Gon-sup (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
  • 강호조 (경상대학교 수의과대학 동물의학연구소) ;
  • 김용환 (경상대학교 수의과대학 동물의학연구소) ;
  • 이후장 (경상대학교 수의과대학 동물의학연구소) ;
  • 김종수 (경상대학교 수의과대학 동물의학연구소) ;
  • 김도경 (경남축산진흥연구소) ;
  • 김은희 (진주국제대학교) ;
  • 박미림 (경상대학교 수의과대학 동물의학연구소) ;
  • 김곤섭 (경상대학교 수의과대학 동물의학연구소)
  • Accepted : 2003.04.07
  • Published : 2003.06.30


The present study was conducted to investigate the preventive effect of feed supplemented with 1.0% oriental herbal medicine feed additives (OHMFA) on the colonization of Salmonella spp. and incidence of death in broiler chickens. The frequency of Salmonella spp. in feces samples treated with OHMFA (25/239; 10.5%) was significantly reduced (p<0.05) than that of control group (83/347; 23.9%). A total of 108 Salmonella spp, belonging to four different serotypes, were isolated from three broiler farms. Among the serotypes isolated, Salmonella typhimurium was the moot prevalent (60.2%), followed by S. enteritidis (20.4%), S. gallinarum (13.0%) and S. pullorum (6.4%). In incidence of death in chickens during experiment, the mortality of rate OHMFA group (3.9%) was significantly lower (p<0.01) than that of control group (5.7%). These results show that the administration of OHMFA may prevent the colonization of Salmonella in chickens.


  1. 강호조, 김용환, 이후장 김종수, 김종섭, 김도경, 김은희, 박미림, 김곤섭. 한방사료 첨가제를 이용한 육계의 Salmonella 방제효과: 항균성 및 장관정착 억제. 대한수의학회지 2003, 43(1), 39-45
  2. 김기석, 이희수, 오인필. 국내 닭에서의 Foul Typhoid 발생 및 원인균 분리. 대한수의학회지 부록. 1993, 33(4), 76
  3. 모인필, 김기석, 권용국. 주요 가금질병 검색 및 역학조사. 농진청 수의과학 연구소 시험연구보고서 pp 318, 1996
  4. Boonmar S., Bangtrankulnonth A., Pornruangwong S., et al. Significant increase in antibiotic resistance of Salmonella isolates from hmnan beings and chicken meat in Thailand. Vet. Microbiol. 1998, 62, 73-80
  5. Chang Y. H. Prevalence of Salmonella spp. in poultry broiler and shell eggs in Korea. J. Food. Prot. 2000, 63 655- 658
  6. Ewing W .H. Edwards and Ewing's Identification of Enterobacteriaceae, 4th ed., Elseviers Science Publishing Co Inc, New York. 1986, pp.29
  7. Gross U., Tschape H., Bednarek L. and Frosch M. Antibiotic resistance in Salmonella enterica serotype typhimurium. Eur. J. Clin. Microbiol. Infect. Dis. 1998, 17, 385-387
  8. Hopper S. A. and Mawer S. Salmonella enteritidis in a commecial layer flock, Vet. Res, 1989, 123, 351-358
  9. Humphrey T. J., Baskerville A., Chart H. and Rowe B. Infection of egg-laying hens with Salmonella enteritidis $PT_4$ by oral inoculation. Vet. Res. 1989, 125, 531-537
  10. Impey C. S., and Mead G. C. Fat of Salmonella in the alimentary tract of chicks pre-treated with a mature caecal microflora to increase colonization resistance. J. Appl. Bact. 1989, 66, 469-4743
  11. Jensen P. Rules reguladon. National poultry improvement plan and auxiliary provisions. Fed Rogister. 1994, 59, 12795-12805
  12. Ji W. J., Jeone M. S., Chung H. C., Choi U. K., Jeong W. H, Kworn K. J., and Chung Y. G. Growth inhibition of water extract cf Schizandra chinensis bullion on the bacteria. J. Food. Hyg. Safety. 2001, 16, 89-95
  13. Kim H. H., Park M. S., Kim S. H., Yu J. Y., Chung B. K. and Lee B. K. Epidemiological characteristics of Salmonella strains isolated in Korea in 1997. Kor. J. Vet. Publ. Hlth. 1998, 22, 253-259
  14. Kim N. H. and Tak R. B. Contamination of Salmonella spp. in chickens slaughtered in Taegu area. Kor. J. Vet Hlth. 1998, 22, 3065-372
  15. Limawongpranee S., Hayashidani H., Okatani A. T., Ono K., Kaneto K. and Ogawa M. Prevalence and persistence of Salmonella in broiler chicken flocks. J. Vet. Med. Sci. 1999, 61, 255-259
  16. Line J. E., Bailey S., Cox N. A. and Froshc M. Yeast treatment to reduce Salmonella and Campylobacter population associated with broiler chickens subjected to transport stress. Poultry Sci. 1997, 76, 1227-1231
  17. Nabbut, N. H. Salmonella serotypes encountered in animal feed additives in Lebanon. Am. J. Vet. Res. 1978, 39, 893-899
  18. Oh G. H. and Choi W. P. Studies on Salmonella isolated from chicks. Korean J. Vet. Res. 1994, 34, 501-510
  19. Oyofo B. A., DeLoach J. R., Corrier D. E., Norman J. D., Ziqrin R. L. and Mollenhauer H. H. Effect of carbohydrates on Salmonella typhimurium colonization in broiler chickens. Avian Dis. 1989, 33, 531-534
  20. Rark K. Y., Yeh J. G. and Park S. G. Characteristics of Salmonella species isolated from poultry. Kor. J. Vet. Publ. Hlth. 1994, 18, 102-116
  21. Roberts D. Salmonella in chilled and frozen chicken. Lancet, 1991, 337, 984-985
  22. Rodrique D. C., Tauxe R. V., and Rowe B. International increase in Salmonella enteritidis, A new pandemic. Epidemiol. Infect. 1990, 105, 21-27
  23. Ruth A. S. plummer, Samantha J. Blissett, and Christine E. R Doddi. Salmonella contamination of retail chicken products sold in the UK. J. Food. Prot. 1995, 58, 843-846
  24. SAS. The SAS system for window. Statistical Analysis System Institute Inc. Carg. NC. 1998
  25. Shivapeasad H. L., Tlmoney J. F., Morales S., Lucio B. and Baker R. C. Pathogenesis of Salmonella enteritidis infection in laying chickens. Studies on egg transmission, clinical signs, feed sheding and serological response, Avian Disease. 1990, 34, 548-555
  26. Tavechio A. T., Ghilardi A. C., Peresi J. T., Fugitara T. D., Yomamine E. K., Jakabi M. and Fernandes S.A. Salmonella serotypes isolated from nonhuman sources in Sao Paulo, Brazil, from 1996 through 2000 J. Food. Prot. 2002, 65, 1041-1044