Antigenicity Study of Nonspecific Immunostimulator BARODON

비특이 면역증강제 BARODON의 항원성시험

  • Jo, Eun-hye (Department of Veterinary Public Health College of Veterinary Medicine, Seoul National University) ;
  • Cho, Sung-dae (Department of Veterinary Public Health College of Veterinary Medicine, Seoul National University) ;
  • Ahn, Nam-shik (Department of Veterinary Public Health College of Veterinary Medicine, Seoul National University) ;
  • Jung, Ji-won (Department of Veterinary Public Health College of Veterinary Medicine, Seoul National University) ;
  • Yang, Se-ran (Department of Veterinary Public Health College of Veterinary Medicine, Seoul National University) ;
  • Park, Joon-suk (Department of Veterinary Public Health College of Veterinary Medicine, Seoul National University) ;
  • Park, Ki-su (Department of Veterinary Public Health College of Veterinary Medicine, Seoul National University) ;
  • Hong, In-sun (Department of Veterinary Public Health College of Veterinary Medicine, Seoul National University) ;
  • Seo, Min-su (Department of Veterinary Public Health College of Veterinary Medicine, Seoul National University) ;
  • Tiep, Nguyeu Ba (Department of Veterinary Public Health College of Veterinary Medicine, Seoul National University) ;
  • Lee, Yong-soon (Department of Veterinary Public Health College of Veterinary Medicine, Seoul National University) ;
  • Kang, Kyung-sun (Department of Veterinary Public Health College of Veterinary Medicine, Seoul National University)
  • 조은혜 (서울대학교 수의과대학 공중보건학교실) ;
  • 조성대 (서울대학교 수의과대학 공중보건학교실) ;
  • 안남식 (서울대학교 수의과대학 공중보건학교실) ;
  • 정지원 (서울대학교 수의과대학 공중보건학교실) ;
  • 양세란 (서울대학교 수의과대학 공중보건학교실) ;
  • 박준석 (서울대학교 수의과대학 공중보건학교실) ;
  • 박기수 (서울대학교 수의과대학 공중보건학교실) ;
  • 홍인선 (서울대학교 수의과대학 공중보건학교실) ;
  • 서민수 (서울대학교 수의과대학 공중보건학교실) ;
  • ;
  • 이영순 (서울대학교 수의과대학 공중보건학교실) ;
  • 강경선 (서울대학교 수의과대학 공중보건학교실)
  • Accepted : 2003.06.02
  • Published : 2003.06.30

Abstract

The antigenicity of nonspecific immunostimulator BARODON$^{(R)}$, a newly developed drug, was investigated by tests for passive cutaneous anaphylaxis (PCA) and active systemic anaphylaxis (ASA) in mice and guinea pigs. In ASA test using guinea pigs, there were no significant clinical symptoms in all individuals of low(0.3%) and high(3%) dose of both groups treated with only BARODON$^{(R)}$ and cotreated with BARODON$^{(R)}$ and adjuvant group. In PCA test, blue spots of Evan's were observed from $2^6$ to $2^{10}$ in homologous group and from $2^2{\sim}2^5$ dilution rate in heterologous group of BSA treated positive control group. However, intradermal sensitization with antiserum obtained from low (0.3%) and high (3%) dose of BARODON$^{(R)}$ only treatment group and treated-with-adjuvant group, followed by intravenous injection of respective antigen and Evan's blue mixture (1:1) showed no blue spot observed. In conclusion, BARODON$^{(R)}$, as showed in ASA and PCA test, did not cause anaphylatic shock when treated 3 and 10 times higher than clinically intended dose, nor induce IgE, so that might not have antigenic properties in mice and guinea pigs.

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