A Study on Sample Preparation for the Analysis of Trace Elements in Foods of Animal Origin by Ultra High Pressure Microwave Digestion

초고압초음파분해법을 이용한 축산물내 미량금속 잔류분석을 위한 시료전처리 방법

  • Lee, Myoung-heon (National Veterinary Research arui Quarantine Services) ;
  • Lee, Hee-su (National Veterinary Research arui Quarantine Services) ;
  • Son, Seong-wan (National Veterinary Research arui Quarantine Services) ;
  • Jung, Gab-soo (National Veterinary Research arui Quarantine Services) ;
  • Park, Jong-myung (National Veterinary Research arui Quarantine Services) ;
  • Kim, Sang-keun (College of Veterinary Medicine, Chungnam National University)
  • Accepted : 2003.08.23
  • Published : 2003.09.25

Abstract

Simple and rapid sample preparation method for trace elements in foods of animal origin using ultra high pressure microwave digestion system (UHP/MDS) and inductively coupled plasma atomic emission spectrometer (ICP/AES) were developed. 1. For the digestion of sample using UHP-MDS, 20% nitric acid (v/v) was the most suitable solvent for the determination of trace elements in foods of animal origin. 2. The optimal digestion conditions for UHP-MDS were as follows: final temperature $180^{\circ}C$, final pressure 400 PSI, and magnetic power 900 W in the solid sample. For the liquid sample final temperature $170^{\circ}C$, final pressure 300 PSI and magnetic power 700 W were optimal conditions. 3. As result of interlaboratory test, the average recovery rate of the for solid sample were 88.3~99.1% for As, 82.4~93.3% for Cd, 89.2~101.2% for Hg and 86.5~93.8% for Pb, respectively. In liquid sample, it were 87.0~96.8% for As, 80.9~96.6% for Cd, 87.5~91.2% for Hg and 91.4~95.5% for Pb, respectively. 4. The average coefficient variation rate were 3.3~15.9% for solid sample and 2.9~10.8% for liquid sample.

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