Determination of Ceftiofur in Bovine Serum by liquid Chromatography-Electrospray Mass Spectrometry

액체크로마토그래프/질량분석기를 이용한 소의 혈청 중 Ceftiofur의 분석

  • Lim, Jong-hwan (College of Veterinary Medicine, Chungnam National University) ;
  • Jang, Beon-su (Medical Science Division, Korea Research Institute of Chemical Technology) ;
  • Park, Byung-kwon (College of Veterinary Medicine, Chungnam National University) ;
  • Yun, Hyo-in (College of Veterinary Medicine, Chungnam National University)
  • 임종환 (충남대학교 수의과대학) ;
  • 장범수 (한국화학연구원 생명의학연구부) ;
  • 박병권 (충남대학교 수의과대학) ;
  • 윤효인 (충남대학교 수의과대학)
  • Accepted : 2003.07.02
  • Published : 2003.09.25

Abstract

This study was aimed to develop a more rapid, simple and sensitive method to determine ceftiofur in bovine serum using LC/MS with electrospray interface. Separation was achieved on the Nova-Pak $C_{18}$ reverse phase column. The mobile phase consisted of 0.1% acetic add in water (A) and acetonitrile (B) and gradiently flowed at the rate of 0.4 mL/min. As a result of analysis of blank muscle samples, matrix interference was not shown. Limit of detection and limit of quantitaion was 5 ng/g and 20 ng/g, respectively. The values of precision and recovery satisfied the guideline of NVRQS. The precision and recovery developed in this method are suitable and sensitive to determine the concentration of ceftiofur in the bovine serum. These results could be applied for the confirmation and quantification in the biofluid.

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