Residue Depletion of Cephalexin in the Flounder (Paralichthys olivaceus)

광어에서 Cephalexin의 잔류 소실에 관한 연구

  • Lim, Jong-hwan (College of Veterinary Medicine, Chungnam National University) ;
  • Jang, Beom-su (Medical Science Division, Korea Research Institute of Chemical Technology) ;
  • Park, Byung-kwon (College of Veterinary Medicine, Chungnam National University) ;
  • Yun, Hyo-in (College of Veterinary Medicine, Chungnam National University)
  • 임종환 (충남대학교 수의과대학) ;
  • 장범수 (한국화학연구원 생명의약연구부) ;
  • 박병권 (충남대학교 수의과대학) ;
  • 윤효인 (충남대학교 수의과대학)
  • Accepted : 2003.07.08
  • Published : 2003.09.25

Abstract

The objective of this study was to investigate the residue depletion of cephaalexin in the flounder (Paralichthys olivaceus) after multiple oral administrations and to establish the appropriate withdrawal time for edible tissues. A highly sensitive and specific method for the determination of cephalexin in the serum of flounder by LC/MS was developed and validated. Mean recoveries from serum were 87.2% (ranged from 81.2% to 94.5%) for cepalexin. Recovery and precision met the criteria for the guideline of residual analysis of veterinary drugs by the National Veterinary Research and Quarantine Service (NVRQS) in Korea. The limit of detection and limit of quantitation of cephalexin were 10 ng/ml and 50 ng/ml, respectively. Residual levels of cephalexin in muscle samples were estimated with 95% tolerance limit and 95% confidence to fall below the MRL after a withdrawal time of 4 days and 5 days for the 40 and 160 mg/kg/day, respectively.

References

  1. Adams. H. R. Veterinary Pharmacology and Therapeutics. 8th ed. Iowa State University Press, Ames, 2001
  2. Agbaba, D., Eric, S., Zivanov, S. D. and Vladimirov, S. HPTLC assay of cephalexin and cefaclor in phar- maceuticals. Biomed. Chromatogr. 1998, 12(3), 133-135
  3. Bergan, T. Pharmacokinetic properties of the cephalosporins. Durgs, 1987, 34(supp1. 2), 64-88
  4. Caprile, K. A. The cephalosporin antimicrobial agents: a comprehensive review. J. Vet. Pharmacol. Therap. 1988, 11, 1-32
  5. Daeseleire, E., De Ruyck, H. and Van Renterghem, R. Confirmatory assay for the simultaneous detection of penicillins and cephalosporins in milk using liquid chromatography/tandem mass spectrometry. Rapid Commun. Mass Spectrom. 2000, 14(15), 1404-1409
  6. EMEA. Note for guidance: approach towards harmonization of withdrawal periods. 1995, EMEA/CVMP/036/95
  7. EMEA. Note for guidance on the establishment of maximum residue limits for Salmonidae and other fin fish. 1998, EMEA/CVMP/153b/97-fina1
  8. Gallo Martinez, L., Campins, F. P. and Sevillano, C. A. Comparison of several methods used for the determination of cephalosporins. Analysis of cephalexin in pharmaceutical samples. J. Pharm. Biomed. Anal. 2002, 29(3), 405-423
  9. Jones, R. N. Antimicrobial activity, spectrum and pharmacokinetics of old and new orally administered cephems. Antimicrob. News. 1988, 5, 1-8
  10. Keever, J., Voyksner, R. D. and Tyczkowska, K. L. Quantitative determination of ceftiofur in milk by liquid chromatography-electrospray mass spectrometry. J. Chromatogr. A. 1998, 794(1-2), 57-62
  11. Lees, P. and Aliabadi, F. S. Rational dosing of antimicrobial drugs: animals versus humans, Int. J. Antimicrob. Agents. 2002, 19(4), 269-84
  12. Lin, C. E., Chen, H. W., Lin, E. C., Lin, K. S., Huang, H. C. Optimization of separation and migration behavior of cephalosporins in capillary zone electro- phoresis. J. Chromatogr. A. 2000, 879(2), 197-210
  13. Li, J., Zhang, Y., Chen, Y., Hao, F. and Chen, Y. E test for studying in vitro activity of seven antimicrobial agents against penicillin-susceptible and penicillin-resistant pneumococci. Chin. Med. J. 2000, 113(7), 628-631
  14. Lim, J. H., Hwang, Y. H., Park, B. K. and Yun, H. I. Combination effects of cephalexin and gentamicin on Edwardsiella tarda and Streptococcus iniae. Int. J. Antimicrob. Agents. 2003, 22, 67-69
  15. Muggleton, P. W., O'Callagan, C. H., Foord, R. D., Kirby, S. M. and Rayan, D. M. Laboatory appraisal of cephalexin. Antimicrob. Agents Chemother. 1968, 8, 353-360
  16. Neu, H. C. Beta-lactam antibiotics: Structural relation- ships affecting in vitro activity and pharmacologic properties. Rev. Infect. Dis. 1986, 8, S237-S259
  17. Noble, W. C. and Kent, L. E. Antibiotic resistance in Staphylococcus intermedius isolated from cases of pyoderma in the dog. Vet. Dermatol. 1992, 3, 71-74
  18. Prescott, J. F. and Baggot, J. D. Antimicrobial Therapy in Veterinary Medicine. Iowa State University Press, Ames, 1993
  19. Riviere, J. E., Webb, A. L. and Craigmill, A. L. A primer on estimating withdrawal times after extralabel drug use, J. Am. Vet. Med. Assoc. 1998, 213, 966-968
  20. Silley, P. and Brewster, G. Kill kinetics of the cephalosporin antibiotics cephalexin and cefuroxime against bacteria of veterinary importance. Vet. Rec. 1998, 123, 343-345
  21. Steppe, M., Prado, M. S., Tavares, M. F., Kedor- Hackmann, E. R. and Santoro, M. I. Determination of cephalexin in oral suspensions by micellar electrokinetic chrornatography. J. Capillary Electrophor. 2002, 7(3-4), 81-86
  22. Tyczkowska, K. L., Voyksner, R. D. and Aronson, A. L. Development of an analytical method for cephapirin and its metabolite in bovine milk and serum by liquid chromatography with UV-VIS detection and confirmation by thermospray mass spectometry. J. Vet. Pharmacol. Ther. 1991, 14(1), 51-60