Residue Depletion of Cephalexin in the Flounder (Paralichthys olivaceus)

광어에서 Cephalexin의 잔류 소실에 관한 연구

  • Lim, Jong-hwan (College of Veterinary Medicine, Chungnam National University) ;
  • Jang, Beom-su (Medical Science Division, Korea Research Institute of Chemical Technology) ;
  • Park, Byung-kwon (College of Veterinary Medicine, Chungnam National University) ;
  • Yun, Hyo-in (College of Veterinary Medicine, Chungnam National University)
  • 임종환 (충남대학교 수의과대학) ;
  • 장범수 (한국화학연구원 생명의약연구부) ;
  • 박병권 (충남대학교 수의과대학) ;
  • 윤효인 (충남대학교 수의과대학)
  • Accepted : 2003.07.08
  • Published : 2003.09.25


The objective of this study was to investigate the residue depletion of cephaalexin in the flounder (Paralichthys olivaceus) after multiple oral administrations and to establish the appropriate withdrawal time for edible tissues. A highly sensitive and specific method for the determination of cephalexin in the serum of flounder by LC/MS was developed and validated. Mean recoveries from serum were 87.2% (ranged from 81.2% to 94.5%) for cepalexin. Recovery and precision met the criteria for the guideline of residual analysis of veterinary drugs by the National Veterinary Research and Quarantine Service (NVRQS) in Korea. The limit of detection and limit of quantitation of cephalexin were 10 ng/ml and 50 ng/ml, respectively. Residual levels of cephalexin in muscle samples were estimated with 95% tolerance limit and 95% confidence to fall below the MRL after a withdrawal time of 4 days and 5 days for the 40 and 160 mg/kg/day, respectively.


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