Expression of VP2 of Aquatic Birnavirus GC-1 Isolated from Rockfish (Sebastes schlegeli), Rearing in Seawater in Korea

국내 해산양식어 조피볼락에서 분리된 수생버나바이러스 GC-1의 VP2 발현

  • Joh, Seong-joon (Avian Disease Division, National Veterinary Research & Quarantine Service) ;
  • Sung, Haan-woo (Avian Disease Division, National Veterinary Research & Quarantine Service) ;
  • Lee, Yun-jeong (Avian Disease Division, National Veterinary Research & Quarantine Service) ;
  • Kim, Jae-hong (Avian Disease Division, National Veterinary Research & Quarantine Service) ;
  • Kang, Shien-young (College of Veterinary Medicine, Chungbuk National University)
  • Accepted : 2003.08.01
  • Published : 2003.09.25

Abstract

The VP2 gene of aquatic birnavirus, Korean isolate (GC-1) was cloned and expressed using the baculovirus expression system. The VP2 gene and VP2 partial gene, which contained a neutralizing epitope, were constructed for recombinant transfer vectors, for baculovirus expression. The expressed recombinant proteins were confirmed by indirect immuno fluorescence antibody (IFA), SDS-PAGE and Western blot. The level of expression was checked at regular time using IFA and Western blot. To measure the neutralizing activity of recombinant proteins against GC-1 strain, the antisera against recombinant proteins were produced by using guinea pigs. The result showed that the antisera neutralized the GC-1 strain. However, the neutralizing titer was higher in antisera against the VP2 gene expressed recombinant protein than that of VP2 partial gene recombinant protein.

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