Porcine Circovirus Infection in Weaned Pigs with Postweaning Multisystemic Wasting Syndrome in Korea

국내 이유자돈의 써코바이러스 감염에 의한 이유후전신소모성 증후군

  • Kim, Jae-hoon (Department of Veterinary Medicine, Cheju National University) ;
  • Roh, In-soon (Pathology Division, National Veterinary Research and Quarantine Service) ;
  • Sohn, Hyun-joo (Pathology Division, National Veterinary Research and Quarantine Service) ;
  • Jean, Young-hwa (Pathology Division, National Veterinary Research and Quarantine Service) ;
  • Hwang, Eui-kyung (College of Life Science & Natural Resources, Sangji University) ;
  • Yoon, Kyoung-jin (Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University)
  • 김재훈 (제주대학교 수의학과) ;
  • 노인순 (국립수의과학검역원 병리과) ;
  • 손현주 (국립수의과학검역원 병리과) ;
  • 진영화 (국립수의과학검역원 병리과) ;
  • 황의경 (상지대학교 생명자원과학대학 동물자원학과) ;
  • 윤경진 (아이오와 주립대학)
  • Accepted : 2003.08.25
  • Published : 2003.09.25


Eight nursery to grower pigs exhibiting weight loss and sudden death were diagnosed as postweaning multisystemic wasting syndrome (PMWS) based on the results of gross findings, histopathology, immunohistochemistry, fluorescent antibody test, virus isolation, PCR, serology, and electron microscopy. Groosly, the pigs had a rough hair coats and were severely emaciated. And moot lymph nodes were pale and enlarged. Lungs were not fully collapsed and exhibited 10 to 40% pale red cranioventral consolidation. Histopathologically, typical lymphohistiocytic interstitial to bronchointerstitial pneumonia, chronic lymphadenitis, severe lymphoid depletion, and basophilic intracytoplasmic inclusions were noted in the most lymphoid tissues. Porcine circovirus panicles were observed in the inguinal lymph node of the pigs by electron microscopy. Porcine circovirus type 2 (PCV2) antigens or viral DNAs were detected in the lesions of all pigs using immunohistochemistry or PCR. Two PCV2 were isolated from a homogenate of pooled lung and lymph node in 2 of the 5 pigs. Additionally, antigens of porcine reproductive and respiratory syndrome virus (PRRSV) and Hemophilus (H.) parasuis were also detected by immunofluorescent antibody test. Serologically, 55% of randomly selected sows and fattening pigs was serum antibody positive to PCV2 by an indirect fluorescent antibody (IFA) test and approximately 18 % of animals in the herd were serologically pooitive by the ELISA kit for PRRSV. To our knowledge, this is the first report of PMWS co-infected with PCV-2, PRRS, and H. parasuis in Korea.


  1. Allan, G. M. and Ellis, J. A. Porcine circovirus: areview. J. Vet. Diagn. Invest. 2000, 12, 3-14
  2. Allan, G. M., Kennedy, S., McNeiIly, F., Foster, J.C., Ellis, J. A., Krakowka, S. J., Meehan, B. M.and Adair, B. M. Experimental reproduction of severewasting disease by co-infection of pig with porcinecircovirus and porcine parvovirus. J. Cornp. Pathol.1999, 121, 1-11
  3. Allan, G. M., McNeaIy, K., Kennedy, S., Dalt, B.,Clark, E. G., Ellis, J. A., Haines, D. M., Meehan, RM. and Adair, B. M. Isolation of porcine circovims-like viruses from piglets with a wasting disease in theUnited States of Amehca and Europe. J. Vet. Diagn.Invest. 1998, 10, 3-10
  4. Balasch, M., Segales, J., Rosell, C., Domingo, M.,Mankertz, A., Umiza, A. and PIana-Duran, J.Experimental inoculation of conventional pigs withtissue homogenates from pigs with postweaning multi-systemic wasting syndrome. J. Comp. Pathol. 1999,121, 139-148
  5. Choi, C. and Chae, C. In-situ hybridization for thedetection of porcine circovirus in pigs with postweaningrnultisystemic wasting syndrome. J. Comp. Path. 1999,121, 265-270
  6. dark, E. G. Post-Weaning multisystemic wastingsyndrome, pp. 499-501. Am. Assoc. Swine Ptact. QuebecCity, 1997
  7. Ellis, J., Hassard, L., dark, E., Harding, J., Allan, G., Wllson, P., Strokappe, J., Martin, K., McNeilly,F., Meehan, B., Todd, D. and Haines, D. Isolation ofcircovirus from lesions of pigs with postweaningmuldsystemic wasting syndrome. Can. Vet. J. 1998, 39,44-51
  8. Ellis, J., Krakowka, S., Larimore, M., Haines, D.,Bratanich, A., dark, E., Allan, G. M., Konoby, C.,Hassard, L., Meehan, B., Martin, K., Harding, J.,Kennedy, S. and McNeilly, F. Reproduction of post-weaning multisystemic wasting syndrome in gnotobioticpigs. J. Vet. Diagn. Invest. 1999, 11, 3-14
  9. Hainel, A. L., Lin, L. L. and Nayar, G. P. S. Nu-cleotide sequence of porcine circovirus associated withpostweaning multisystemic wasting syndrome in pigs.J. Viml. 1998, 72, 5262-5267
  10. Harding, J. C. and Clark, E. G. Recoenizing anddiagnosing postwsaning miiltisystemic wasdng syndrome(PMWS). Swine Health Prod. 1998, 5(5), 201-203
  11. Harding, J. C. S., Clark, E. G., Strokappe, J. H.,Willson, P. I., and Ellis, J. A. Postweaning multi-systemic wasting syndrome: Epidemiology and clinicalpresentation. Swine Health Prod. 1998, 6(6), 249-254
  12. Hamis, P. Field Studies of Postweaning MultisystemicWasting Syndrome, PP. 5-7. In 6th Annual SwineDisease Conference for Swine Practitioners, Ames, 1998
  13. Hwang, E. K., Kim, J. H., Kim, B. H., Park, C. K.and Choi, S. H. Infectious agents associated withswine abortions and stillbirths in Korea. RDA J. Vet.Sci. 1998, 40, 48-53
  14. Kim, J. H., Hwang, E. K., Kim, Y. J. and Sohn, H.J. Pathologic studies in piglets naturally infected withporcine reproductive and respiratory syndrome virus.Korea J. Vet. Pathol. 1997, 1, 125-133
  15. Kiupel, M., Stevenson, G. W., Mittal, S. K., dark,E. G. and Haines, D. M. Circoviras-like viral associateddisease in weaned pigs in Indiana. Vet. Pathol. 1998,35, 303-307
  16. LeCann, P., Albina, E., Madec, F., Cariole, R. and Jesdn, A. Hglet wasdng dsease. Vet. Rec. 1997, 141, 660
  17. Meehan. B. M., McNeiUy, F., Todd, D., Kennedy,&, Jewhurst, V. A., Hlis, L &, HiKsard, L E, Clark,E. GU Haines D. M, and Allan, G. M Characterizationof novel circovirus DNAs associated with wastingsyndromes in pigs. J. Cien. Virol. 1998, 79, 2171-2179
  18. Monxew, L, Sirinarumitr, T., Sorden, S. D., Halbur,P. G., Morgan, M. K., Yoon, K. J. and Paul, P. S.Detection of a novel strain of porcine circovirus mpigs with postwsaning multisystemic wasting syndrome.J. din. Microbiol. 1998, 36, 2535-2541
  19. Pogranichnyy, R. M., Yoon, K. J., Harms, P. A.,Swenson, S. L., Ziminennan, J. J. and Sorden, S.D. Characterization of immune response of young pigsto porcine circovirus type 2 infection. Viral Immunol.2000, 13(2), 143-153
  20. Rosell, C., Segales, J., Plana-Duran, J., Balasch, M.,Rodriguez-Arrioja, G. M., Kennedy, S., Allan, G.M., McNeiIly, F., Latimer, K. S. and Domingo, M.Pathological, immunohistochemical, and In-situ hybridi-zation studies of natural cases of postweaning multi-systemic wasting syndrome (PMWS) in pigs. J. Comp.Path. 1999, 120, 59-78
  21. Segales, J., Sitjar, M., Domingo, M., Dee, S., delPozo, M., Novel, R., Sacristan, C., de Las Heras,A., Ferro, A. and Latimer, K. S. First repon ofpost-weaning multisystemic wasting syndrome in pigsin Spain. Vet. Rec. 1997, 141, 600-601
  22. Sorden, S. D. Postweaning Multisystemic WastingSyndrome (PMWS) - Trends in Diagnosdcs, PP. 39-42.In 7th Aimual Swine Disease Conference for SwinePractitioners, Ames, 1999
  23. Sorden, S. D., Hanns, P. A., Nawagitgul, P.,Cavanaugh, D. and Paul, P. S. Development of apolyclonal-antibody-based immunohistochemistrymethod for the detection of type 2 porcine circovirusin formalin-fixed, Paraffin-embedded tissue. J. Vet. Diagn.Invest. 1999, 11, 528-530