Rapid and Easy Detection of Porcine Epidemic Diarrhea Virus (PEDV) by in situ Hybridization

In Situ Hybridization에 의한 돼지 유행성설사증 (Porcine Epidemic Diarrhea)의 진단

  • Park, Nam-yong (College of Veterinary Medicine, Chonnam National University) ;
  • Cho, Ho-seong (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Tae-ju (College of Veterinary Medicine, Chonnam National University) ;
  • Park, Young-seok (Department of Companion Laboratory Animal Science, Kongju University)
  • 박남용 (전남대학교 수의과대학) ;
  • 조호성 (전남대학교 수의과대학) ;
  • 김태주 (전남대학교 수의과대학) ;
  • 박영석 (공주대학교 특수동물학과)
  • Accepted : 2003.06.04
  • Published : 2003.09.25

Abstract

Molecular diagnostic techniques have been used to identify porcine epidemic diarrhea virus (PEDV), a causative agent of acute enteritis in swine, but they were difficult to be petformed and time-consuming. To detect PEDV in a rapid and easy way, we developed biotinylated cDNA probe for N gene encoding the nucleoproteins of PEDV. Formalin-fixed and paraffin-embedded tissues from 24 naturally infected pigs were used for the experiment. The ISH produced a positive reaction in all cases. When intestinal tissues were hybridized with PEDV probe, strong signals were seen in the villus enterocytes of the jejunum and ileum. Hybridization signals were also found in the duodenum from one pig and in colon from dnother. In conclusion, ISH with a biotinylated cDNA probe was provided to be a useful diagnostic method for detecting PEDV effectively in routinely processed tissue sections.

Acknowledgement

Supported by : 농촌진흥청

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