Apoptosis of Germ Cells after Vasectomy in Rats

수컷 랫드에서 졍관절제술에 의한 생식세포의 Apoptosis

  • Choi, Jong-yun (College of Veterinary Medicine, Chungnam National University) ;
  • Cho, Sung-whan (College of Veterinary Medicine, Chungnam National University) ;
  • Ryu, Si-yoon (College of Veterinary Medicine, Chungnam National University) ;
  • Jee, Young-heun (Department of Veterinary Medicine, Cheju National University) ;
  • Lee, Geun-jwa (Chungnam Livestock & Veterinary Service Institute) ;
  • Son, Hwa-young (College of Veterinary Medicine, Chungnam National University)
  • Accepted : 2003.08.02
  • Published : 2003.09.25

Abstract

The pathological mechanism of impaired spermatogenesis after vasectomy has not been completely investigated. In this study, we examined pathological changes of the testis and the Fas-Fas ligand (FasL) mediated signaling pathway in apoptotic germ cell death after vasectomy in rats. Ten-weeks old Sprague-Dawley rats were underwent bilateral vasectomy and sacrificed after 1 day, 2 days, 3 days, 5 days, 1 week, 2 weeks, and 4 weeks of surgery and the testes were removed. Histopathological evaluation of spermatogenesis was performed by hematoxylin-eosin and periodic acid-Schiff-hematoxylin staining. To elucidate the pathophysiology of seminiferous tubule damage, terminal dUTP nick end labeling staining, electrophoresis assay of DNA fragmentation, and Western blotting analysis for Fas-FasL were performed. Relative weights of testes were decreased from 5 days after vasectomy. Germ cell degeneration were first found in the spermatogonia and spermatocytes at stages I-VI, and XII-XIV seminiferous tubules. Mean incidence of apoptotic germ cells after vasectomy progressively increased to peak in 5 days, and then gradually decreased to the control levels in 2 weeks after vasectomy. The expression of Fas-FasL reached maximum level at 5 days after vasectomy and then declined. In conclusion, impaired spermatogenesis after vasectomy associated with an increase in germ cell apoptasis, which is partly mediated by the activation of Fas-FasL.

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