오존 가스가 피부창상의 치유에 미치는 영향

The Effects of Ozone Gas on Wound Healing in Skin

  • 이수진 (충남대학교 수의과대학) ;
  • 이재일 (충남대학교 수의과대학) ;
  • 김명철 (충남대학교 수의과대학) ;
  • 홍성혁 (충남대학교 수의과대학) ;
  • 김덕환 (충남대학교 수의과대학) ;
  • 조성환 (충남대학교 수의과대학)
  • Lee, Soo-jin (College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Jae-il (College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Myung-cheol (College of Veterinary Medicine, Chungnam National University) ;
  • Hong, Sung-hyuk (College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Duck-hwan (College of Veterinary Medicine, Chungnam National University) ;
  • Cho, Sung-whan (College of Veterinary Medicine, Chungnam National University)
  • 심사 : 2003.08.09
  • 발행 : 2003.09.25

초록

This study was performed to determine the optimal dose of ozone for wound healing. Twenty Korean black goats were allocated to 4 groups. The skin wound with $1{\times}1cm$ was induced over the hoof. For 5 minutes per day, 0.1 ppm (Group I), 1 ppm (Group II) and 6 ppm (Group III) ozone gas was applied. In control group, 5 ml of normal saline was applied daily on the wound. Wound square was determined using color image analyzer at day 0, 4 and 14. Soft tissue depth of wound was determined using radiograph. Before the application of ozone gas, $1{\times}1cm$ of skin tissue was resected and observed for histopathologic findings. To determine the healing effect, $2{\times}1cm$ of wound tissue was resected and observed for histopathologic findings at day 14. In mean squares of wound, all experimental groups revealed more reduced mean squares than control group at day 4, and especially Group II (p<0.05) and Group III (p<0.05) revealed significant reduction. At day 14, Group I and Group II (p<0.05) revealed more reduced mean squares than control group, but Group III revealed less reduced mean squares than control group. In soft tissue depth of wound, all experimental groups and control group revealed swelling at day 4, compared with day 0. At day 14, all experimental groups and control group revealed reduction, compared with day 4, and especially Group II (p<0.05) revealed significant reduction. In histopathological findings, inflammation findings in epidermis and dermis, hemorrhagic finding in epidermis were revealed in control group. In Group I, inflammation finding was reduced, compared with control group. Formation of scab and many reepithelialization of epidermis were revealed. In Group II, inflammation findings in epidermis and dermis which were observed in control group were not found, normal skin tissue was revealed. In Group III, inflammation findings in epidermis and dermis were revealed, reepithelialization of epidermis was not founded. Slow healing process was observed, compared with Group I and II. It was concluded that Group II has excellent promotion effect of wound healing.

참고문헌

  1. 권오경. 가초 창상 치유에 대한 항균 연고제의 효과. 한곽임상수의학회지. 1993, 10, 55-60
  2. 박재헌, 권오경, 남치주. 가토 창상에 미치는 yeast glucan의 효과. 한국임상수의학회지. 1994, 11, 427- 435
  3. 양현국, 권오경, 남치주. Aloe가 토끼 창상 치유에 미치는 영향. 한국임상수의학회지 1995, 12, 215- 220
  4. 임성준, 권오경, 장광호. chIorhexidine gluconate 및povidone iodine이 가토의 창상 치유에 미치는 효과.한국임강수의학회지. 1992, 9, 17-21
  5. 최순철, 권오경, 남치주. Epidermal growth factor가 가토 창상 치유에 미치는 효과. 한국임상수의학회지. 1995. 12, 221-226
  6. 한국공기청정연구조합. 오존을 이용한 살균 및 탈취 기술 개박에 관한 연구. 통강 산업부. 서울. 1995, pp.1-3
  7. Beligotskii, N. N. The use of ozone for treating suppurative wounds. Klin. Khir. 1994, 4, 52-54
  8. Berton, A. L., Sullins, K. E., Stashak, T. S. and Norridin, R. W. Effect of wound location and the use of topical collagen gel on exuberant granuladon tissue fonnation and wound healing in the horse and pony. Am. J. Vet. Res. 1985, 46, 1438-1444
  9. Ceccherelli, F., Gagliardi, G., Faggian, L., Loprete, F. and Giron, G. Analgesic effect of subcutaneous administration of oxygen-ozone. A blind study in the rat on the modulation of the capsaicin induced ederna. Acupuncture & Electro-Therapeutics Research. 1998, 23, 171-184
  10. Cooper, M. L., Laxer, J. A. and Hansbrough, J.F. The cytotoxic effects of commonly used topicalantimicrobial agents on human fibroblasts and kera-tinocytes. J. Trauma. 1994, 31, 775-784
  11. D'Erme. Ozone therapy in lumbar sciatic pain. LaRadiologia Medica, 1998, 95, 21-24
  12. Ehrlich, H. P., Tarver, H. and Hunt, T. K. Effect ofvitamin A and glucoconicoids upon inflammation andcollagen synthesis. Ann. Sug. 1972, 177, 222-227
  13. EIIis, H. J. C. R and Belcher, H. Soinatropin andwound healing after injury. J. Clin. Endocnn. Metabol.1990, 70, 939-943
  14. Gierek, L. A. Preliminary report on using generalozone therapy in diseases of the posterior segment ofthe eye. Klinika Oczna. 1992, 94, 139-140
  15. Gorbunov, S. Total ozone therapy of trophic ulcers oflower extremities in elderly patients, In Proceedings,The 2nd International Symposium on Qzone Applications,pp. 24-26. Havana, Cuba, 1997
  16. Harari, J. Surgical Complications and Wound Healingin thi Simll AniiTHl Placdce. W B Saunders, Philadelphia,1998, PP. 63-88
  17. Horst, W. Subattnospheric Q2/03-treattnent of therapy-resistent wounds and ulcerations. Qzonachrichten. 1985,4, 1-2
  18. Johnsenm, D. E. Wound healing in skin. Plastic andreconstructive surgery. Vet. C1in. Nor. Amer. 1990, 20,1-25
  19. Ka-shiwagi, K., Saito, K., Wang, V. D., Takahashi,H., Ishijima, K. and Tsukahara, S. Safety of ozomtedsolution as an antiseptic of the ocular surface prior toophthalmic surgery. Ophthalndogica. 2001, 215, 351-356
  20. Kotov, S. A. Ozone therapy of migraine. Zh. Ne.Psikhiatr In. S. S. Konakova. 2000, 100, 35-37
  21. Kudiiavtsev, E. P., Miroshin, S. I., Semenov, S. V.,Snigorenko, A. S. and Sidorovich, I. A. Ozonethempy of diffuse peritonitis in the early postoperativeperiod. Khirurgiia. 1997, 8, 36-41
  22. Lee, A. H., Swaim, S. F., McGure, J. A. and Hughes,K. S. Effect of chlorhexidine diacetate, Povidoneiodine and polyhydroxidine on wound healing in dog.J. Am. Ani. Hosp. Asso. 1998, 24, 77-84
  23. Malanchuk, V. A., Gorshevikova, E. V. and Kopchak,A. V. Antirnicrobial action of ozone in the treatmentof mandibular fracture. Klin. Khir. 2000, 10, 43-46
  24. Malanchuk, V. A., kupchak, A. V., Savitskaia, 1.M. and Stupina, A. S. Pathomorphological aspects ofthe inflammatory process in soft tissues during the ozoneapplication in the experiment. Klin. Khir. 2000, 10,52-55
  25. Melton, C. E. Effect of long-term exposure to lowlevels of ozone: a review. Aviat. Space Environ. Med.1982, 53, 105-111
  26. Minaini, S., Okamoto, Y., IVlatsiiha.shi, A. and Eguchi,H. Clinical application of chitin and its derivates tosmall animals. J. A. S. V. A. 1994, 34, 5-19
  27. Mittler, S., Hedrick, D., King, M. and Gaynor, A.Toxicity of ozone. I. Acute toxicity. Ind. Med. Surg.1956, 25, 301-306
  28. Mittler, S., King, M. and Burkhardt, B. Toxicity ofozone, In. Chronic toxicity. AMA Arch Ind. Health.1957, 15, 191-197
  29. Ogata, A. Intramammary application of ozone therapyto acute clinical mastitis in dairy cows. J. Vet. Med.Sci. 2000, 62, 681-686
  30. Ovchinnikov, L. M. and Sin'kov, E. V. Use of gaseousozone and ozonized solutions in the treatment ofchronic suppuradve otitis msdia. Vestn. Otorinolaringol.1998, 1, 11-12
  31. Probst, C. W. and Bright, R. M. Wound healing, inSlatter D, ed Textbook of Small Animal Surgery. 1stW. B. Saunders, Philadelphia, 1985, 23-37
  32. Roehm, G. N., Hadley, G. and Menzel, D. B. The influence of vitamin E on lung fatty acids of ratsexposed to ozone. Arch. Environ. Health. 1972, 24,237-242
  33. Sabo, J. C. and Oberlander, L. Acceleration of open wound healing by cartilage. Arch Surg. 1964, 90,414-417
  34. Scrollavezza, P. Ozone treatment in mastitis, metritis and retention of fetal membranes in the dairy cow. In Proceedings of the 2nd International Symposium on Ozone Applications, pp. 1-8. Havana, Cuba, 1997
  35. Shakov, V. L. and Edeleva, A. N. Reasons for the application of medical ozone in the treatment of chronic purulent mesotympanitis. Vestn. Otorinolaringol. 1999, 2, 48-49
  36. Smith, K. P. and Zardiackas, L. D. Cortisone, vitamin A and wound healing: The importance of measuring wound surface area. J. Surg. Rec. 1998, 40, 120-125
  37. Stephens, F. 0., Dunphy, J. E. and Hunt, T. K. Effect of delayed administration of corticosteroids on wound contraction. Ann. Surg. 1971, 173, 214-218
  38. Swaim, S. F., Henderson, R. A. and Pidgeon, R. S. Srnall animal wound management. Lea & Febiger, 1990, 44-49
  39. Swaim, S. F. and Lee, A. H. Topical wound medications: A review. J. Am. Vet. Mid. Assoc. 1987, 190, 1588-1593
  40. Thwaiyes, M. and Dean, S. Chronic leg ulcers. Ozone and other factors affecting healing. Aust. Fam. Physician. 1985, 14, 292-298
  41. Vasil'ev. The antibacterial and irnmunocorrective action of ozone therapy in peritonitis. Vestn. Khir. Im. I. I.eniGrek. 1995, 154, 56-60
  42. Viebahn, R. The use of ozone in medicine. Karl F. Haug Publishers, Heidelberg, 1944, 7, 26-30, 38-65, 68, 90
  43. Xie, W. The role of ozone solution on debridement and sterilization of burn wound. Zhonghua Shao Shang Za Zhi. 2000, 16, 163-165