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Multianalyte Sensor Array using Capillary-Based Sample Introduction Fluidic Structure: Toward the Development of an "Electronic Tongue"

  • Sohn, Young-Soo (The University of Texas at Austin, Department of Electrical and Computer Engineering) ;
  • Anslyn, Eric V. (Department of Chemistry and Biochemistry) ;
  • McDevitt, John T. (Department of Chemistry and Biochemistry) ;
  • Shera, Jason B. (Department of Chemistry and Biochemistry) ;
  • Neikirk, Dean P. (The University of Texas at Austin, Department of Electrical and Computer Engineering)
  • Published : 2004.09.30

Abstract

A micromachined fluidic structure for the introduction of liquid samples into a chip-based sensor array composed of individually addressable polymeric microbeads has been developed. The structure consists of a separately attached cover glass, a single silicon chip having micromachined channels and microbead storage cavities, and a glass carver. In our sensor array, transduction occurs via colorimetric and fluorescence changes to receptors and indicator molecules that are covalently attached to termination sites on the polymeric microbeads. Data streams are acquired for each of the individual microbeads using a CCD. One of the key parts of the structure is a passive fluid introduction system driven only by capillary force. The velocity of penetration of a horizontal capillary for the device having a rectangular cross section has been derived, and it is quite similar to the Washburn Equation calculated for a pipe with a circular cross section having uniform radius. The test results show that this system is useful in a ${\mu}$-TAS and biomedical applications.

References

  1. G. Kovacs, Micromachined Transducers Sourcebook, McGraw-Hill, New York, 1998
  2. S. Savoy, J. J. Lavigne, S.-J. Yoo, J. Wright, M. Rodriguez, A. Goodey, B. MeDoniel, J. T. McDevitt, E. V. Anslyn, J. B. Shear, A. Ellington, and D. P. Neikirk, 'Solution-based analysis of multiple analytes by a sensor array: toward the development of an 'Electronic Tongue' ', Proc. ofSPIE Conf. on Chemical Microsensors and Applications, Vol. 3539, Boston, pp. 17-26, 1998 https://doi.org/10.1117/12.333750
  3. A. Goodey, J. Lavigne, S. Savoy, M. Rodriguez, C. Theodore, A. Tsao, G. Simmons, J. Wright, S.-J. Yoo, Y. Sohn, E. Anslyn, J. Shear, D. Neikirk, and J. McDevitt, 'Development of multianalyte sensor arrayscomposed of chemical derivatized polymeric microspheres localized in micromachined cavities', J. Am. Chem. Soc., vol. 123, pp. 2559-2570, 2001 https://doi.org/10.1021/ja003341l
  4. J. J. Lavigne, S. Savoy, M. B. Clevenger, J. E. Ritchie, B. McDoniel, S.-J. Yoo, E. V. Anslyn, J. T. McDevitt, J. B. Shear, and D. P. Neikirk, 'Solutionbased analysis of multiple analytes by a sensor array: toward the development of an 'Electronic Tongue' ', J. Am. Chem. Soc., vol. 120, pp. 6429-6430, 1998 https://doi.org/10.1021/ja9743405
  5. N. Christodoulides, M. Tran, P. N. Floriano, M. Rodriguez, A. Goodey, M. Ali, D. Neikirk, and J. T. McDevitt, 'A microchip-based multianalyte assay system for the assessment of cardiac risk', Anal. Chem., vol. 74, pp. 3030-3036, 2002 https://doi.org/10.1021/ac011150a
  6. M. Bodanszky, Principles of Peptide Synthesis, Springer-Verlag, Berlin, 2nd edn., 1993
  7. C.-J. Kim, 'Microfluidic using the surface tension force in microscale', Proc. of SPIE Conf. on Microfluidic Devices and Systems III, Vol. 4177, Santa Clara, pp. 49-54, 2000 https://doi.org/10.1117/12.395656
  8. F. W. Went, 'The size of man', American Scientist, vol. 56, pp. 400-413, 1968
  9. E. D. Washburn, 'The dynamics of capillary flow', Physical Review, vol. 17, pp. 273-283, 1921 https://doi.org/10.1103/PhysRev.17.273
  10. Youngsoo Sohn, John Levigne, Dr. Yi Deng, Adrian Goodey, Marc Rodriquez, Andrew Tsao, Theodore E. Curey, Dean P. Neikirk, Eric Anslyn, John McDevitt, and Jason Shear, 'Micromachined multianalyte sensors: towards an electronic tongue', NASA NanoSpace 2000, International Conference on Integrated Nano/Microtechnology for Space Applications, League City, TX, Jan. 23-28, 2000