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The Effect of Hirudin Herbal-acupuncture on Neurotransmitters against Middle Cerebral Artery Occulsion(MCAO) Rats

Hirudin 약침(藥鍼)이 뇌허혈(腦虛血)을 유발(誘發)시킨 흰쥐의 신경전도물질(神經傳達物質)에 미치는 영향(影響)

  • Suk, Jae-Wook (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Hanny University) ;
  • Jung, Tae-Young (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Hanny University) ;
  • Leem, Seong-Cheol (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Hanny University) ;
  • Seo, Jeong-Chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Hanny University) ;
  • Han, Sang-Won (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Hanny University)
  • 석재욱 (대구한의대학교 한의과대학 침구학교실) ;
  • 정태영 (대구한의대학교 한의과대학 침구학교실) ;
  • 임성철 (대구한의대학교 한의과대학 침구학교실) ;
  • 서정철 (대구한의대학교 한의과대학 침구학교실) ;
  • 한상원 (대구한의대학교 한의과대학 침구학교실)
  • Published : 2004.06.25

Abstract

Objective : This experimental studies were performed in order to prove the effect of Hirudin Herbal-acupuncture by using rats that had neuronal damage due to the Middle Cerebral Artery Occulsion(MCAO). Method : We observed the change of extracellular concentrations(${\mu}M$) of dopamine, DOPAC, HVA, HIAA, glutamate, aspartate, GABA, glysine, taurine, alanine, and tyrosine as extracted by vivo microdialysis, in the Hirudin Herbal-acupuncture administrated rats($240{\sim}260g$, Sprague-Dawley) subjected to the MCAO. The dialysates were extracted three times before the MCAO and six times after the MCAO every 20 minutes, and analysed by highperformance liquid chromatography(HPLC). Results : Hirudin Herbal-acupuncture significantly inhibited glutamate, aspartate, and tyrosine which are stimulant neurotransmitters at brain ischemia, and it significantly decreased glycine, GABA, taurine, and alanine which are inhibitory neurotransmitters at brain ischemia. Conclusion : Hirudin Herbal-acupuncture may prevent delayed neuronal death(DND) in selectively vulnerable focal areas of the brain effectively.

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